Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)

Revision as of 16:16, 4 August 2008

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence
puc19	positive control	A	CFU
L104	Strong rbs - lasR activator D129 (BV) - D114 (BI)
L105	Strong promoter - ECFP D123 (BV) - D130 (BI)
L106	Strong promoter - gfp Tripart D123 (BV) - D131 (BI)
L107	Strongest promoter - ECFP D103 (BV) - D131 (BI)
L108	Strong promoter - gfp Tripart D103 (BV) - D130 (BI)
L110	Medium promoter - gfp Tripart D124 (BV) - D131 (BI)
L111	Weak promoter - ECFP D104 (BV) - D130 (BI)
L112	Weak promoter - gfp D104 (BV) - D131 (BI)
L115	pLas - ECFP D105 (BV) - D130 (BI)
L116	pLas - gfp Tripart D105 (BV) - D131 (BI)
L117	Double Terminator - yfp D125 (FV) - D117 (FI)
L118	Double Terminator - rfp D125 (FV) - D121 (FI)
L119	Double Terminator - lasR activator + LVA D125 (FV) - D113 (FI)
L121	Strong promoter - gfp tripart D106 (BV) - D130 (BI)
L124	Strongest RBS - rfp D102 (BV) - D122 (BI)
L125	Strongest RBS - yfp D102 (BV) - D118 (BI)				L100	A	0 CFU	0 CFU
L113		K	few
L114		K	medium
L120		A	few
L122		A	medium
L123		A	0 CFU
L126		A	a lot

@@ Line 17: / Line 17: @@
 |align="center"|
 |-
+|align="center"|L104
+|align="center"|Strong rbs - lasR activator
+<br>D129 (BV) -  D114 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L105
+|align="center"|
+Strong promoter - ECFP
+<br>D123 (BV) - D130 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L106
+|align="center"|
+Strong promoter - gfp Tripart
+<br>D123 (BV) - D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L107
+|align="center"|
+Strongest promoter - ECFP
+<br>D103 (BV) - D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L108
+|align="center"|
+Strong promoter -  gfp Tripart
+<br>D103 (BV) - D130 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L110
+|align="center"|
+Medium promoter - gfp Tripart
+<br>D124 (BV) - D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L111
+|align="center"|
+Weak promoter - ECFP
+<br>D104 (BV) - D130 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L112
+|align="center"|
+Weak promoter - gfp
+<br>D104 (BV) - D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L115
+|align="center"|
+pLas - ECFP
+<br>D105 (BV) - D130 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L116
+|align="center"|
+pLas - gfp Tripart
+<br>D105 (BV) - D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L117
+|align="center"|
+Double Terminator - yfp
+<br>D125 (FV) - D117 (FI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L118
+|align="center"|
+Double Terminator - rfp
+<br>D125 (FV) - D121 (FI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L119
+|align="center"|
+Double Terminator - lasR activator + LVA
+<br>D125 (FV) - D113 (FI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L121
+|align="center"|
+Strong promoter - gfp tripart
+<br>D106 (BV) - D130 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L124
+|align="center"|
+Strongest RBS - rfp
+<br>D102 (BV) - D122 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|-
+|align="center"|L125
+|align="center"|
+Strongest RBS - yfp
+<br>D102 (BV) - D118 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|align="center"|
 |align="center"|L100
 |align="center"|