Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)
(Results of the transformation)
(Results of the transformation (bis-the good one to leave in august 2))
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=== Results of the transformation (bis-the good one to leave in august 2)===
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=== Results of the transformation ===

Revision as of 18:05, 4 August 2008

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Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

Amp 608 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

Amp > 4000 GFP positive OK
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

Kana 9 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

Kana 9 To do again
L120

tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)

Amp 74 To do again
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

Amp 4 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

Amp > 4000 To do again
L126

Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)

Amp 944 To do again