Team:Paris/August 2

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{{Paris/Calendar_Links|August 1|August 3}}
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== Transformation of 1st August ==
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=== Results of the transformation ===
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{| border="1"  
{| border="1"  
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|align="center"|'''Nb colonies observed'''
|align="center"|'''Nb colonies observed'''
|align="center"|'''Fluorescence'''
|align="center"|'''Fluorescence'''
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|align="center"|'''Comments'''
|-
|-
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|align="center"| -
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|align="center"|puc19
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|align="center"|positive control
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|align="center"|positive control
-
|align="center"|A
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|align="center"|Amp
-
|align="center"|2.800 CFU
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|align="center"|
-
|align="center"|2.800 CFU
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|align="center"|-
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|align="center"|OK
|-
|-
-
|align="center"| -
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|align="center"|L100
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|align="center"|negative control
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|align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI) 
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|align="center"|A
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|align="center"|Amp
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|align="center"|0 CFU
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|align="center"|608
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|align="center"|0 CFU
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|align="center"|No
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|align="center"|OK
|-
|-
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|align="center"|2E_SP 1000
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|align="center"|L101
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|align="center"|B0034
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|align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI) 
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|align="center"|A
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|align="center"|Amp
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|align="center"|few
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|align="center"|> 4000
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|align="center"|few
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|align="center"|GFP positive
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|align="center"|OK
|-
|-
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|align="center"|10F_SP 1001
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|align="center"|L113
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|align="center"|R0079
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|align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI)
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|align="center"|A
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|align="center"|Kana
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|align="center"|medium
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|align="center"|9
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|align="center"|medium
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|align="center"|
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|align="center"|
|-
|-
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|align="center"|4F_SP 1013
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|align="center"|L114
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|align="center"|C0079
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|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
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|align="center"|K
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|align="center"|Kana
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|align="center"|few
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|align="center"|9
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|align="center"|few
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|align="center"|
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|align="center"|
|-
|-
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|align="center"|6H_SP 1001
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|align="center"|L120
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|align="center"|C0179
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|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
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|align="center"|A
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|align="center"|Amp
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|align="center"|medium
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|align="center"|74
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|align="center"|medium
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|align="center"|
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|align="center"|
|-
|-
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|align="center"|6B_SP 1001
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|align="center"|L122
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|align="center"|S03154
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|align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI)
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|align="center"|A
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|align="center"|Amp
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|align="center"|0 CFU
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|align="center"|4
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|align="center"|0 CFU
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|align="center"|No
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|align="center"|OK
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|-
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|align="center"|10F_SP 1002
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|align="center"|L123
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|align="center"|J23101
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|align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI)
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|align="center"|A
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|align="center"|Amp
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|align="center"|a lot
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|align="center"|> 4000
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|align="center"|a lot
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|align="center"|No
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|align="center"|OK
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|-
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|align="center"|11F_SP 1002
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|align="center"|L126
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|align="center"|J23109
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|align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
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|align="center"|A
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|align="center"|Amp
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|align="center"|a lot
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|align="center"|944
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|align="center"|a lot
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|align="center"|No
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|-
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|align="center"|OK
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|align="center"|4C_SP 1000
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|align="center"|R0040
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|align="center"|A
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|align="center"|2 CFU
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|align="center"|2 CFU
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-
|-
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|align="center"|2G_SP 1000
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|align="center"|C0040
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|align="center"|A
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|align="center"|0 CFU
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|align="center"|0 CFU
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-
|-
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|align="center"|1C_SP 1008
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|align="center"|PSB1A3
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|align="center"|A
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|align="center"|few
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|align="center"|few
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-
|-
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|align="center"|7G_SP 1013
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|align="center"|E1010
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|align="center"|K
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|align="center"|medium
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|align="center"|medium
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-
|-
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|align="center"|7E_SP 1001
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|align="center"|E0040
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|align="center"|A
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|align="center"|2 CFU
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|align="center"|2 CFU
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-
|-
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|align="center"|2F_SP 1016
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|align="center"|E0030
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|align="center"|K
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|align="center"|a lot
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|align="center"|a lot
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|}
|}
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[[Image:transfo.jpg|frame|center|L101]]
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==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

Latest revision as of 17:09, 13 August 2008

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Transformation of 1st August

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
puc19 positive control Amp - OK
L100 rbs TetR - ECFP
D110 (BV) - D130 (BI) 		Amp 608 No OK
L101 rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		Amp > 4000 GFP positive OK
L113 AracpBAD - ecfp
D126 (BV) - D130 (BI) 		Kana 9
L114 AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		Kana 9
L120 tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		Amp 74
L122 RBS-lasI - ecfp
D107 (BV) - D130 (BI) 		Amp 4 No OK
L123 RBS lasI - GFP tripart
D107 (BV) - D131 (BI) 		Amp > 4000 No OK
L126 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		Amp 944 No OK
L101

==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

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