Team:Paris/August 2

From 2008.igem.org

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{{Paris/Calendar_Links|August 1 |August 03}}
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{{Paris/Calendar_Links|August 1|August 3}}
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== Transformation of 1st August ==
=== Results of the transformation ===
=== Results of the transformation ===
Line 12: Line 16:
|align="center"|'''Comments'''
|align="center"|'''Comments'''
|-
|-
-
|align="center"|L100
+
|align="center"|puc19
-
|align="center"|rbs TetR - ECFP
+
|align="center"|positive control  
-
<br>D110 (BV) - D130 (BI) 
+
|align="center"|Amp
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L101
+
-
|align="center"|rbs TetR - GFP tripart
+
-
<br>D110 (BV) -  D131 (BI) 
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L102
+
-
|align="center"|Strong rbs - YFP
+
-
<br>D129 (BV) -  D118 (BI) 
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L103
+
-
|align="center"|Strong rbs - GFP
+
-
<br>D129 (BV) -  D122 (BI) 
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L104
+
-
|align="center"|Strong rbs - lasR activator
+
-
<br>D129 (BV) -  D114 (BI) 
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L105
+
-
|align="center"|
+
-
Strong promoter - ECFP
+
-
<br>D123 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center"|
+
|align="center"|
|align="center"|
 +
|align="center"|-
|align="center"|OK
|align="center"|OK
|-
|-
-
|align="center"|L106
+
|align="center"|L100
-
|align="center"|
+
|align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI)
-
Strong promoter - gfp Tripart
+
|align="center"|Amp
-
<br>D123 (BV) - D131 (BI)
+
|align="center"|608
-
|align="center"|
+
|align="center"|No
-
|align="center"|
+
-
|align="center"|
+
|align="center"|OK
|align="center"|OK
|-
|-
-
|align="center"|L107
+
|align="center"|L101
-
|align="center"|
+
|align="center"|rbs TetR - GFP tripart<br>D110 (BV) - D131 (BI)
-
Strongest promoter - ECFP
+
|align="center"|Amp
-
<br>D103 (BV) - D131 (BI)
+
|align="center"|> 4000
-
|align="center"|
+
|align="center"|GFP positive
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L108 n°2 (the right one)
+
-
|align="center"|
+
-
Strong promoter -  gfp Tripart
+
-
<br>D103 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
|align="center"|OK
|align="center"|OK
|-
|-
-
|align="center"|L110
+
|align="center"|L113
 +
|align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI)
 +
|align="center"|Kana
 +
|align="center"|9
|align="center"|
|align="center"|
-
Medium promoter - gfp Tripart
 
-
<br>D124 (BV) - D131 (BI)
 
|align="center"|
|align="center"|
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|OK
 
|-
|-
-
|align="center"|L111
+
|align="center"|L114
 +
|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
 +
|align="center"|Kana
 +
|align="center"|9
|align="center"|
|align="center"|
-
Weak promoter - ECFP
 
-
<br>D104 (BV) - D130 (BI)
 
|align="center"|
|align="center"|
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L112
 
-
|align="center"|
 
-
Weak promoter - gfp
 
-
<br>D104 (BV) - D131 (BI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L115
 
-
|align="center"|
 
-
pLas - ECFP
 
-
<br>D105 (BV) - D130 (BI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L116
 
-
|align="center"|
 
-
pLas - gfp Tripart
 
-
<br>D105 (BV) - D131 (BI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L117
 
-
|align="center"|
 
-
yfp - Double Terminator
 
-
<br>D125 (FV) - D117 (FI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L118
 
-
|align="center"|
 
-
rfp - Double Terminator
 
-
<br>D125 (FV) - D121 (FI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L119
 
-
|align="center"|
 
-
lasR activator + LVA - Double Terminator
 
-
<br>D125 (FV) - D113 (FI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
|-
|-
|align="center"|L120
|align="center"|L120
 +
|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
 +
|align="center"|Amp
 +
|align="center"|74
|align="center"|
|align="center"|
-
tetR repressible - ECFP
 
-
<br>D106 (BV) - D130 (BI)
 
|align="center"|
|align="center"|
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
|-
|-
-
|align="center"|L121
+
|align="center"|L122
-
|align="center"|
+
|align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI)
-
Strong promoter - gfp tripart
+
|align="center"|Amp
-
<br>D106 (BV) - D130 (BI)
+
|align="center"|4
-
|align="center"|
+
|align="center"|No
-
|align="center"|
+
-
|align="center"|
+
|align="center"|OK
|align="center"|OK
-
|-
 
-
|align="center"|L122
 
-
|align="center"|
 
-
RBS-lasI - ecfp
 
-
<br>D107 (BV) - D130 (BI)
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|To do again
 
|-
|-
|align="center"|L123
|align="center"|L123
-
|align="center"|
+
|align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI)
-
RBS lasI - ecfp
+
|align="center"|Amp
-
<br>D107 (BV) - D131 (BI)
+
|align="center"|> 4000
-
|align="center"|
+
|align="center"|No
-
|align="center"|
+
|align="center"|OK
-
|align="center"|
+
-
|align="center"|To do again
+
|-
|-
-
|align="center"|L124
+
|align="center"|L126
-
|align="center"|
+
|align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
-
Strongest RBS - rfp
+
|align="center"|Amp
-
<br>D102 (BV) - D122 (BI)
+
|align="center"|944
-
|align="center"|
+
|align="center"|No
-
|align="center"|
+
|align="center"|OK
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L125
+
-
|align="center"|
+
-
Strongest RBS - yfp
+
-
<br>D102 (BV) - D118 (BI)
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|To do again
+
|}
|}
 +
 +
[[Image:transfo.jpg|frame|center|L101]]
 +
==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

Latest revision as of 17:09, 13 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →


Transformation of 1st August

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
puc19 positive control Amp - OK
L100 rbs TetR - ECFP
D110 (BV) - D130 (BI) 		Amp 608 No OK
L101 rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		Amp > 4000 GFP positive OK
L113 AracpBAD - ecfp
D126 (BV) - D130 (BI) 		Kana 9
L114 AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		Kana 9
L120 tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		Amp 74
L122 RBS-lasI - ecfp
D107 (BV) - D130 (BI) 		Amp 4 No OK
L123 RBS lasI - GFP tripart
D107 (BV) - D131 (BI) 		Amp > 4000 No OK
L126 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		Amp 944 No OK
L101

==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

Retrieved from "http://2008.igem.org/Team:Paris/August_2"