Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)

Latest revision as of 17:09, 13 August 2008

Transformation of 1st August

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
puc19	positive control	Amp		-	OK
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)	Amp	608	No	OK
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)	Amp	> 4000	GFP positive	OK
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)	Kana	9
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)	Kana	9
L120	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)	Amp	74
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)	Amp	4	No	OK
L123	RBS lasI - GFP tripart D107 (BV) - D131 (BI)	Amp	> 4000	No	OK
L126	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	Amp	944	No	OK

L101

==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

@@ Line 21: / Line 21: @@
 |align="center"|
 |align="center"|-
-|align="center"|ok
+|align="center"|OK
 |-
 |align="center"|L100
@@ Line 27: / Line 27: @@
 |align="center"|Amp
 |align="center"|608
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |-
 |align="center"|L101
@@ Line 35: / Line 35: @@
 |align="center"|> 4000
 |align="center"|GFP positive
-|align="center"|ok
+|align="center"|OK
 |-
 |align="center"|L113
@@ Line 42: / Line 42: @@
 |align="center"|9
 |align="center"|
-|align="center"|To do again
+|align="center"|
 |-
 |align="center"|L114
@@ Line 49: / Line 49: @@
 |align="center"|9
 |align="center"|
-|align="center"|To do again
+|align="center"|
 |-
 |align="center"|L120
@@ Line 56: / Line 56: @@
 |align="center"|74
 |align="center"|
-|align="center"|To do again
+|align="center"|
 |-
 |align="center"|L122
@@ Line 62: / Line 62: @@
 |align="center"|Amp
 |align="center"|4
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |-
 |align="center"|L123
-|align="center"|RBS lasI - ecfp<br>D107 (BV) - D131 (BI)
+|align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI)
 |align="center"|Amp
 |align="center"|> 4000
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |-
 |align="center"|L126
@@ Line 76: / Line 76: @@
 |align="center"|Amp
 |align="center"|944
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |}
+[[Image:transfo.jpg|frame|center|L101]]
 ==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed.