Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)
(Results of the transformation)
(Results of the transformation)
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|align="center"|'''Fluorescence'''
|align="center"|'''Fluorescence'''
|align="center"|'''Comments'''
|align="center"|'''Comments'''
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|-
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|align="center"|puc19
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|align="center"|positive control 
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|align="center"|Amp
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|align="center"|
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|align="center"|-
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|align="center"|ok
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|align="center"|L100
|align="center"|L100

Revision as of 16:43, 6 August 2008

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Transformation of 1st August

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
puc19 positive control Amp - ok
L100 rbs TetR - ECFP
D110 (BV) - D130 (BI)
Amp 608 To do again
L101 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Amp > 4000 GFP positive OK
L113 AracpBAD - ecfp
D126 (BV) - D130 (BI)
Kana 9 To do again
L114 AracpBAD - gfp tripart
D126 (BV) - D131 (BI)
Kana 9 To do again
L120 tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)
Amp 74 To do again
L122 RBS-lasI - ecfp
D107 (BV) - D130 (BI)
Amp 4 To do again
L123 RBS lasI - ecfp
D107 (BV) - D131 (BI)
Amp > 4000 To do again
L126 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Amp 944 To do again