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Team:Paris/August 21
From 2008.igem.org
(Difference between revisions)
(→Screening of the cloning of E0240 and FlhDC+promotor) |
(→Screening of the cloning of E0240 and FlhDC+promotor) |
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==PCR screening== | ==PCR screening== | ||
| - | reaction mixture (25 µL) | + | '''reaction mixture''' (25 µL) |
*12,5 µL Quick load PCR Mixture 2X | *12,5 µL Quick load PCR Mixture 2X | ||
*0,5 µL O18 | *0,5 µL O18 | ||
*0,5 µL O19 | *0,5 µL O19 | ||
*11,5 µL water | *11,5 µL water | ||
| - | PCR screening programm | + | '''PCR screening programm''' |
*elongation time: 1 min 30 | *elongation time: 1 min 30 | ||
*primers: O18 and O19 | *primers: O18 and O19 | ||
Revision as of 13:46, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
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