Team:Paris/August 21
From 2008.igem.org
(Difference between revisions)
(→Screening of the cloning of E0240 and FlhDC+promotor) |
(→Screening of the cloning of E0240 and FlhDC+promotor) |
||
Line 127: | Line 127: | ||
| | | | ||
| | | | ||
- | |colspan="13"| | + | |colspan="13"|1192 bp |
| | | | ||
|- | |- | ||
Line 202: | Line 202: | ||
| | | | ||
| | | | ||
- | |colspan="13"| | + | |colspan="13"|1403 bp |
| | | | ||
|- | |- |
Revision as of 14:06, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
|