Team:Paris/August 21
From 2008.igem.org
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(→Electrophoresis) |
(→Screening of the cloning of E0240 and FlhDC+promotor) |
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- | =Screening of the cloning of E0240 and FlhDC+promotor= | + | =='''Ligation'''== |
+ | [[Team:Paris/Notebook/Protocols#Ligation |Protocol]] | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Ligation Name''' | ||
+ | |'''Vector Name''' | ||
+ | |'''Volume Vector (µL)''' | ||
+ | |'''Insert''' | ||
+ | |'''Volume Insert (µL)''' | ||
+ | |- | ||
+ | |L160 | ||
+ | |D166 | ||
+ | |3.63 | ||
+ | |D132 | ||
+ | |2.03 | ||
+ | |- | ||
+ | |L161 | ||
+ | |D167 | ||
+ | |4.00 | ||
+ | |D132 | ||
+ | |2.01 | ||
+ | |- | ||
+ | |Control L160 | ||
+ | |D166 | ||
+ | |3.63 | ||
+ | | - | ||
+ | | - | ||
+ | |- | ||
+ | |Control L161 | ||
+ | |D167 | ||
+ | |4.00 | ||
+ | | - | ||
+ | | - | ||
+ | |} | ||
+ | |||
+ | |||
+ | ='''Screening of the cloning of E0240 and FlhDC+promotor'''= | ||
We obtained single colonies with the dilution 100. | We obtained single colonies with the dilution 100. |
Revision as of 19:25, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Ligation
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
Electrophoresis
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