Team:Paris/August 22
From 2008.igem.org
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|'''Vector digestion''' | |'''Vector digestion''' | ||
|'''Vector description''' | |'''Vector description''' | ||
- | |'''Vector | + | |'''C° Vector (µg/mL)''' |
- | |'''Vector | + | |'''V. Vector (µl)''' |
|'''Insert digestion''' | |'''Insert digestion''' | ||
|'''Insert description''' | |'''Insert description''' | ||
- | |'''Insert | + | |'''C° Insert (µg/mL)''' |
- | |'''Insert | + | |'''V. Insert (µl)''' |
|'''Product description''' | |'''Product description''' | ||
|'''Antibiotic''' | |'''Antibiotic''' |
Revision as of 14:32, 6 September 2008
Analysis of the transformant of FlhDC+promotor
PCRPCR screening programm
Digestiontotal volume reaction (30 µL)
Incubation 2h55 at 37°C and then 20 min at 65°C. Electrophoresis
Construction of pFlgA - GFP GeneratorAim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240) DigestionDigestion
Gel Extraction
Measurement of the concentration of D168 purifiedProtocol (it's same that for Miniprep)
Ligation
Construction for FIFOAim : Construction of pFlgA - YFP tripart (+/- LVA) "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) Transformation of the ligations we did yesterday
Construction for synchronizationTransformation of the ligations we did yesterday
LigationTransformation
Promoter characterization plasmidsTransformation of ligations from August 20thTop 10 cells were used
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