Team:Paris/August 22
From 2008.igem.org
(Difference between revisions)
(→Transformation of ligations from August 20th) |
(→Electrophoresis) |
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|'''measured size''' | |'''measured size''' | ||
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- | |'''1 kb'''<br>3 kb | + | |style="background: #ff6d73" |'''1 kb'''<br>3 kb |
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- | |0,3 kb<br>1,8 kb | + | |style="background: #ff6d73" |0,3 kb<br>1,8 kb |
- | |0,3 kb<br>1,8 kb | + | |style="background: #cbff7B"| 0,3 kb<br>1,8 kb |
- | |0,9 kb<br>2 kb | + | |style="background: #ff6d73" |0,9 kb<br>2 kb |
- | |2 kb | + | |style="background: #ff6d73" |2 kb |
- | |2 kb | + | |style="background: #ff6d73" |2 kb |
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|} | |} | ||
- | <br>'''Results''': The clones tested didn't have the insert. | + | <br> '''Results''': The clones tested didn't have the insert. |
='''Construction of pFlgA - GFP Generator'''= | ='''Construction of pFlgA - GFP Generator'''= |
Revision as of 14:36, 6 September 2008
Analysis of the transformant of FlhDC+promotor
PCRPCR screening programm
Digestiontotal volume reaction (30 µL)
Incubation 2h55 at 37°C and then 20 min at 65°C. Electrophoresis
Construction of pFlgA - GFP GeneratorAim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240) DigestionDigestion
Gel Extraction
Measurement of the concentration of D168 purifiedProtocol (it's same that for Miniprep)
Ligation
Construction for FIFOAim : Construction of pFlgA - YFP tripart (+/- LVA) "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) Transformation of the ligations we did yesterday
Construction for synchronizationTransformation of the ligations we did yesterday
LigationTransformation
Promoter characterization plasmidsTransformation of ligations from August 20thTop 10 cells were used
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