Team:Paris/August 25

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Contents

Construction for Synchronization

Transformation of the ligations we did yesterday

Ligation Name Description Antibio
L159 D125 (FV) + D109 (FI) Kan
Control L159 D125 Kan
L162 D107 (BV) + D163 (BI) Amp
Control L162 D107 Amp
L163 D110 (BV) + D163 (BI) Amp
Control L163 D110 Amp


Construction of pFlgA - GFP Generator

Aim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0430/E0432) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png


Results of the transformation we did the day before yesterday

Ligation name Description Antibio Number Colonies observed Fluorescence Comments
Ligations
L164 D168(FV) - D132(FI)
pFlgA - gfp generator 		Amp 125 No ok Controls
TL164 D168(FV) Amp 8 No ok
Positive Control pUC19 Amp 2000 (efficiency 2.10^8) No OK 
=> Need to screen to know which clones we can use for the  of  pFlgA promotor caracteristion.


Cloning of EnvZ*

KR000225.jpg
Gel after excision
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