Team:Paris/August 26
From 2008.igem.org
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==Resuts== | ==Resuts== | ||
- | [[Image:KR000239.jpg| | + | [[Image:KR000239.jpg|thumb|Results of the cloning of EnvZ* and OmpR*]] |
===Electrophoresis settings=== | ===Electrophoresis settings=== | ||
*Gel 1% agar | *Gel 1% agar |
Revision as of 16:56, 26 August 2008
Extraction of EnvZ* and OmpR* from E. coli genomeName of the PCR
ProtocolProtocol
PCR Programme
ResutsElectrophoresis settings
Results of the electrophoresis
Conclusion : All the PCR worked perfectly well ! Cleaning of the PCR productsDigestion of the PCR productsPCRPCR1
Program: Gradient Vf=20µL PCR2
Program: promoter Vf=50µL PCR3
Program: PCRFliA1 Program: PCRFliA2 Program: PCRFliA3
Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation
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