Team:Paris/August 27

From 2008.igem.org

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{{Paris/Calendar_Links|August 26|August 28}}
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='''Construction of pFlgA - GFP Generator'''=
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Aim : Construction of ''' "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
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==Digestion==
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==='''Measurement of the concentration of D187 purified'''===
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[[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]]
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{|border="1" style="text-align: center"
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|'''Digestion Name'''
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|'''Concentration (µg/mL)'''
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|'''Ratio 260/280'''
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|-
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|D187
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|
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|
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|}
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==Ligation==
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[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
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{|border="1" style="text-align: center"
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|'''Ligation Name'''
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|'''Vector Name'''
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|'''Volume Vector (µL)'''
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|'''Insert'''
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|'''Volume Insert (µL)'''
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|-
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|L1
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|D187
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|
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|D186
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|
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|-
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|Control L1
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|D187
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|
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| -
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| -
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|}

Revision as of 17:55, 26 August 2008

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Contents

Construction of pFlgA - GFP Generator

Aim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L1 D187 D186
Control L1 D187 - -
Retrieved from "http://2008.igem.org/Team:Paris/August_27"