Team:Paris/August 27
From 2008.igem.org
(Difference between revisions)
(New page: {{Paris/Calendar_Links|August 26|August 28}}) |
|||
Line 1: | Line 1: | ||
{{Paris/Calendar_Links|August 26|August 28}} | {{Paris/Calendar_Links|August 26|August 28}} | ||
+ | |||
+ | |||
+ | ='''Construction of pFlgA - GFP Generator'''= | ||
+ | |||
+ | Aim : Construction of ''' "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
+ | |||
+ | ==Digestion== | ||
+ | ==='''Measurement of the concentration of D187 purified'''=== | ||
+ | |||
+ | [[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]] | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Digestion Name''' | ||
+ | |'''Concentration (µg/mL)''' | ||
+ | |'''Ratio 260/280''' | ||
+ | |- | ||
+ | |D187 | ||
+ | | | ||
+ | | | ||
+ | |} | ||
+ | |||
+ | ==Ligation== | ||
+ | [[Team:Paris/Notebook/Protocols#Ligation |Protocol]] | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Ligation Name''' | ||
+ | |'''Vector Name''' | ||
+ | |'''Volume Vector (µL)''' | ||
+ | |'''Insert''' | ||
+ | |'''Volume Insert (µL)''' | ||
+ | |- | ||
+ | |L1 | ||
+ | |D187 | ||
+ | | | ||
+ | |D186 | ||
+ | | | ||
+ | |- | ||
+ | |Control L1 | ||
+ | |D187 | ||
+ | | | ||
+ | | - | ||
+ | | - | ||
+ | |} |
Revision as of 17:55, 26 August 2008
Construction of pFlgA - GFP GeneratorAim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240) DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
|