Team:Paris/August 27
From 2008.igem.org
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===Cleaning of the digestion products=== | ===Cleaning of the digestion products=== | ||
+ | [[Team:Paris/Notebook/Protocols#Purification_.28Kit_Promega.29|Standard protocol.]] | ||
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==Ligation== | ==Ligation== | ||
===Determination of the concentration of DNA=== | ===Determination of the concentration of DNA=== | ||
===Protocol of ligation=== | ===Protocol of ligation=== |
Revision as of 13:25, 27 August 2008
Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ* in pSB1A2Transformation results
PCR screening
Cloning of OmpR*DigestionDetermination of the concentration of DNAWe used the biophotometer
Name of the digestions
Protocol of digestion
Cleaning of the digestion productsLigationDetermination of the concentration of DNAProtocol of ligation |