Team:Paris/August 27

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Contents

1 Construction of pFlgA - GFP Generator
- 1.1 Digestion
  - 1.1.1 Measurement of the concentration of D187 purified
- 1.2 Ligation

Construction of pFlgA - GFP Generator

Aim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240)

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name	Concentration (µg/mL)	Ratio 260/280
D187

Ligation

Ligation Name	Vector Name	Volume Vector (µL)	Insert	Volume Insert (µL)
L1	D187		D186
Control L1	D187		-	-

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