Team:Paris/August 28

From 2008.igem.org

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(→Transformation of OmpR*)
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{{Paris/Calendar_Links|August 27|August 29}}
{{Paris/Calendar_Links|August 27|August 29}}
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=Transformation of OmpR*=
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='''Transformation of OmpR*'''=
='''Construction of pFlhB - mRFP Tripart (LVA+)'''=
='''Construction of pFlhB - mRFP Tripart (LVA+)'''=

Revision as of 14:50, 28 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Transformation of OmpR*

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 D186
Control L168 D166 -
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