Team:Paris/August 28
From 2008.igem.org
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==Digestion== | ==Digestion== | ||
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+ | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]] | ||
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Revision as of 12:44, 29 August 2008
Transformation of OmpR*Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth. DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion
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