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Team:Paris/August 28
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{{Paris/Calendar_Links|August 27|August 29}} | {{Paris/Calendar_Links|August 27|August 29}} | ||
| - | =Transformation of OmpR*= | + | ='''Transformation of OmpR*'''= |
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| + | =Cloning of EnvZ*= | ||
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| + | The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. | ||
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| + | ==Digestion== | ||
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| + | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]] | ||
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| + | {|border="1" style="text-align: center" | ||
| + | |'''Digestion n°''' | ||
| + | |'''Name''' | ||
| + | |'''volume of DNA digested''' | ||
| + | |'''total volume reaction''' | ||
| + | |'''enzymes used''' | ||
| + | |- | ||
| + | |D159 | ||
| + | |PCR147 (EnvZ*) | ||
| + | |3 µL | ||
| + | |30 µL | ||
| + | |XbaI & PstI | ||
| + | |- | ||
| + | |D116 | ||
| + | |MP108 (lasR-pSB1A2) | ||
| + | |2 µL | ||
| + | |30 µL | ||
| + | |XbaI & PstI | ||
| + | |} | ||
| + | <br> | ||
| + | '''Purification''' | ||
| + | *EnvZ*: directly by the Qiagen kit | ||
| + | *pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | ||
| + | |||
| + | =Freezer empty= | ||
| + | [[Team:Paris/Notebook/Protocols#Digestion |Protocol]] | ||
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| + | We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and [[Team:Paris/Notebook/Freezer#Digestion Products |D125]]. | ||
Latest revision as of 19:09, 9 September 2008
Transformation of OmpR*Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion
Freezer empty |
