Team:Paris/August 28

From 2008.igem.org

(Difference between revisions)
(→Measurement of the concentration of D187 purified)
Line 10: Line 10:
==Digestion==
==Digestion==
==='''Measurement of the concentration of D187 purified'''===
==='''Measurement of the concentration of D187 purified'''===
 +
 +
[[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Digestion Name'''
 +
|'''Concentration (µg/mL)'''
 +
|'''Ratio 260/280'''
 +
|-
 +
|D187
 +
|
 +
|
 +
|}
 +
 +
==Ligation==
 +
[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Ligation Name'''
 +
|'''Vector Name'''
 +
|'''Volume Vector (µL)'''
 +
|'''Insert'''
 +
|'''Volume Insert (µL)'''
 +
|-
 +
|L168
 +
|D187
 +
|
 +
|D186
 +
|
 +
|-
 +
|Control L168
 +
|D166
 +
|
 +
| -
 +
|}

Revision as of 20:17, 27 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Transformation of OmpR*

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 D186
Control L168 D166 -
Retrieved from "http://2008.igem.org/Team:Paris/August_28"