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Team:Paris/August 28
From 2008.igem.org
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==Digestion== | ==Digestion== | ||
| - | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]] | + | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]][[Image:EnvZ pSB1A2 after.JPG|thumb|Excision of the band corresponding to pSB1A2]] |
| - | [[Image:EnvZ pSB1A2 after.JPG|thumb|Excision of the band corresponding to pSB1A2]] | + | |
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
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*EnvZ*: directly by the Qiagen kit | *EnvZ*: directly by the Qiagen kit | ||
*pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | *pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | ||
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| + | ='''Digestion'''= | ||
| + | [[Team:Paris/Notebook/Protocols#Digestion |Protocol]] | ||
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| + | We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and [[Team:Paris/Notebook/Freezer#Digestion Products |D125]]. | ||
Revision as of 14:37, 29 August 2008
Transformation of OmpR*Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion 
Digestion |
