"
Team:Paris/August 4
From 2008.igem.org
(Difference between revisions)
(New page: {{Paris/Calendar_Links|August 3|August 5}} == '''PCR Screening of the Ligation Transformants'''== Use of 8 clones of Ligation transformants n°L100 for screening PCR ===Protocol of s...) |
(→Results) |
||
| Line 47: | Line 47: | ||
| - | ===Results=== | + | ===Results for L100=== |
| + | |||
| + | L100= D106 + D120 = '''RBS-tetR-ECFP-Ter''' [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | ||
| - | |||
{| border="1" | {| border="1" | ||
|align="center"|'''Band''' | |align="center"|'''Band''' | ||
| - | |align="center"| | + | |align="center"| 1-->8 |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
|- | |- | ||
|align="center"|'''Expected size''' | |align="center"|'''Expected size''' | ||
| - | | | + | |align="center"|1042 pb |
|- | |- | ||
|align="center"|'''Measured size''' | |align="center"|'''Measured size''' | ||
| - | |align="center"| | + | |align="center"|1100 pb |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
|- | |- | ||
| - | |colspan="8"|[[Image: | + | |colspan="8"|[[Image:KR000101_1.jpg|thumb|'''Gel : L100(1-8)''']] |
|} | |} | ||
Revision as of 16:45, 4 August 2008
|
PCR Screening of the Ligation TransformantsUse of 8 clones of Ligation transformants n°L100 for screening PCR
Protocol of screening PCR
Conditions of electrophoresis
Results for L100L100= D106 + D120 = RBS-tetR-ECFP-Ter
|
|||||||||||||||||||||||||||||
