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Team:Paris/August 6
From 2008.igem.org
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(→Analysis of yesterday's gel extraction (assay to quantify the DNA contained in the eluate)) |
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===Digestion=== | ===Digestion=== | ||
| + | |||
| + | For each sample (MP123 and PCR products of MP124, MP125, MP126 and MP127 previously purified by QIAquick Gel Extraction): | ||
| + | * 10 µL of DNA (Miniprep eluate or Gel extraction eluate of the PCR products) | ||
| + | * 12,5 µL water | ||
| + | * 2,5 µL buffer 2 | ||
| + | * 0,25 µL BSA | ||
| + | * 1 µL EcoRI | ||
| + | The reaction was incubated 1h30 at 37°C, then 1h30 more after adding 1 µL of SpeI. | ||
===Analysis of yesterday's gel extraction (assay to quantify the DNA contained in the eluate)=== | ===Analysis of yesterday's gel extraction (assay to quantify the DNA contained in the eluate)=== | ||
The samples analysed are the minipreps eluate of MP123 and the Gel Extraction eluate of the PCR products from MP124, MP125, MP126 and MP127. | The samples analysed are the minipreps eluate of MP123 and the Gel Extraction eluate of the PCR products from MP124, MP125, MP126 and MP127. | ||
Revision as of 12:04, 6 August 2008
Plasmid extractionDigestionFor each sample (MP123 and PCR products of MP124, MP125, MP126 and MP127 previously purified by QIAquick Gel Extraction):
The reaction was incubated 1h30 at 37°C, then 1h30 more after adding 1 µL of SpeI. Analysis of yesterday's gel extraction (assay to quantify the DNA contained in the eluate)The samples analysed are the minipreps eluate of MP123 and the Gel Extraction eluate of the PCR products from MP124, MP125, MP126 and MP127. |
