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Team:Paris/August 7
From 2008.igem.org
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E0240 and pSB3K3 are ok : there is a lot of single colonies | E0240 and pSB3K3 are ok : there is a lot of single colonies | ||
S120 and S121 : there is a problem, there is nothing on the plates. We have to check whether those strains are really resistant to Amp. | S120 and S121 : there is a problem, there is nothing on the plates. We have to check whether those strains are really resistant to Amp. | ||
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==Results of the PCR we did last night== | ==Results of the PCR we did last night== | ||
[[image:KR000117.jpg|thumb|PCR with gradient to amplify pflhDC]] | [[image:KR000117.jpg|thumb|PCR with gradient to amplify pflhDC]] | ||
[[image:KR000116.jpg|thumb|Standart PCR to amplify pflgA, pflgB and pflhB]] | [[image:KR000116.jpg|thumb|Standart PCR to amplify pflgA, pflgB and pflhB]] | ||
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| + | ==Transformations== | ||
| + | ===Protocol=== | ||
| + | Use of TOP10 chemically competentcells | ||
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| + | * Defroze competent cells on ice during 5' | ||
| + | *Add | ||
Revision as of 13:33, 7 August 2008
Result of the isolation of coloniesE0240 and pSB3K3 are ok : there is a lot of single colonies S120 and S121 : there is a problem, there is nothing on the plates. We have to check whether those strains are really resistant to Amp. Results of the PCR we did last night  TransformationsProtocolUse of TOP10 chemically competentcells
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