Team:Paris/August 7

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Result of the isolation of colonies

E0240 and pSB3K3 are ok : there is a lot of single colonies S120 and S121 : there is a problem, there is nothing on the plates. We have to check whether those strains are really resistant to Amp.

Results of the PCR we did last night

PCR with gradient to amplify pflhDC

Standart PCR to amplify pflgA, pflgB and pflhB

Transformations

Protocol

Use of TOP10 chemically competentcells

Defroze competent cells on ice during 5'
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