Team:Paris/July 14

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(Culture of Cells)
 
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{{Paris/Calendar_Links|July 13|July 15}
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{{Paris/Calendar_Links|July 13|July 15}}
==Results of the transformation==
==Results of the transformation==
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* Positive control : puc19 (1µl; 10pg/ml) --> No transformation
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* Positive control : pUC19 (1µl; 10pg/ml) --> No transformation
* Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
* Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
* Biobricks 2008 : --> No transformation
* Biobricks 2008 : --> No transformation
           Conclusion : Cells are competents, because there is positive transformation
           Conclusion : Cells are competents, because there is positive transformation
           Hypothesis : problem with the protocol of DNA extraction from the registery 2008
           Hypothesis : problem with the protocol of DNA extraction from the registery 2008
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==Culture of Cells==
==Culture of Cells==
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* Culture of DH5ɑ in 5ml of LB without Antibiotics
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* Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C
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==Preparation of medium and solutions==
==Preparation of medium and solutions==

Latest revision as of 10:08, 16 July 2008

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Results of the transformation

  • Positive control : pUC19 (1µl; 10pg/ml) --> No transformation
  • Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
  • Biobricks 2008 : --> No transformation
          Conclusion : Cells are competents, because there is positive transformation
          Hypothesis : problem with the protocol of DNA extraction from the registery 2008

Culture of Cells

  • Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C

Preparation of medium and solutions

  • 1L of CCMB80
  • 500ml of SOB ( 2 x 250ml)
  • 500ml of SOC (2 x 250ml)
  • 100ml of MgCl2 (1M)
  • 150ml of CaCl2 (1M)
  • 200ml of KOAC (1M)