Team:Paris/July 19
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===Electrophoresis=== | ===Electrophoresis=== | ||
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Band 3 : B0034 clone 2 -> ok | Band 3 : B0034 clone 2 -> ok | ||
Band 4 : R0040 clone 1 -> ok | Band 4 : R0040 clone 1 -> ok | ||
Band 5 : R0040 clone 2 -> problem | Band 5 : R0040 clone 2 -> problem | ||
Band 6 : R0040 ? -> problem | Band 6 : R0040 ? -> problem | ||
+ | |||
+ | [[Image:screen.jpg|right]] | ||
+ | {| border="1" | ||
+ | |align="center"|'''Band''' | ||
+ | |align="center"|'''Biobricks''' | ||
+ | |align="center"|'''Clone''' | ||
+ | |align="center"|'''Expected size''' | ||
+ | |align="center"|'''Measured size''' | ||
+ | |- | ||
+ | |align="left"|2 | ||
+ | |align="left"|Ladder | ||
+ | |align="center"| | ||
+ | |align="center"|100pb | ||
+ | |align="center"| | ||
+ | |- | ||
+ | |align="left"|3 | ||
+ | |align="left"|B0034 | ||
+ | |align="center"|1 | ||
+ | |align="center"|250 pb | ||
+ | |align="center"|nothing | ||
+ | |- | ||
+ | |align="left"|4 | ||
+ | |align="left"|B0034 | ||
+ | |align="center"|2 | ||
+ | |align="center"|250 pb | ||
+ | |align="center"|~250pb | ||
+ | |- | ||
+ | |align="left"|5 | ||
+ | |align="left"|R0040 | ||
+ | |align="center"|1 | ||
+ | |align="center"|292 pb | ||
+ | |align="center"|~300pb | ||
+ | |- | ||
+ | |align="left"|6 | ||
+ | |align="left"|R0040 : Problem !!! | ||
+ | |align="center"|2 | ||
+ | |align="center"|292 pb | ||
+ | |align="center"|~250pb | ||
+ | |- | ||
+ | |align="left"|7 | ||
+ | |align="left"|R0040 : Problem !!! | ||
+ | |align="center"|? | ||
+ | |align="center"|292 pb | ||
+ | |align="center"|~250pb | ||
+ | |- | ||
+ | |} |
Revision as of 14:52, 21 July 2008
MiniPrepThe protocol of MiniPrep of Quiagen has been done on all the clones cultivated on the 18th.
J23109 clones 1,2,5
Quantitative analysis of ADNgel 1
We see bad results for B0034 (bands 10 to 12), R0040 (band 15). We'll try again! gel 2We see bad results for J23109 (bands 1 to 3), C0179 (bands 14 to 16). We'll try again! Screening R0040 and B0034PCRFor 1 tube :
25µL Quick Load Program SCREENIN ElectrophoresisBand 3 : B0034 clone 2 -> ok Band 4 : R0040 clone 1 -> ok Band 5 : R0040 clone 2 -> problem Band 6 : R0040 ? -> problem
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