Team:Paris/July 20

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Design of the oligo

for flagellum and mutagenesis of FliA

Culture (in 5ml LB)

only for the minipreps with doubt : B0034 (3 clones) R0040 (2 clones) J23109 (3 clones) C0179 (3 clones)

Summary of the Biobricks that we owns

B0015 : double terminator B0030 : RBS.1 (strong) B0034 : RBS C0079 : LasR activator E0030 : YFP E0040 : GFP E0422 : CFP E1010 : mRFP J23100 : constitutive promotor family member J23101 : constitutive promotor family member J23107 : constitutive promotor family member R0079 : promoter (LasR & PAI regulated)