Team:Paris/July 20

Design of the oligo

for flagellum and mutagenesis of FliA

Culture (in 5ml LB)

only for the minipreps with doubt :

B0034 (3 clones)

R0040 (2 clones)

J23109 (3 clones)

C0179 (3 clones)

Summary of the Biobricks that we owns

B0015  : double terminator

B0030  : RBS.1 (strong)

B0034  : RBS

C0079  : LasR activator

E0030  : YFP

E0040  : GFP

E0422  : CFP

E1010  : mRFP

J23100 : constitutive promotor family member

J23101 : constitutive promotor family member

J23107 : constitutive promotor family member

R0079  : promoter (LasR & PAI regulated)