Team:Paris/July 22

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(Culture of Stable strain with biobricks 2008 (in 6ml LB with adaptated antibiotics))
(Culture of Stable strain with biobricks 2008 (in 6ml LB with adaptated antibiotics))
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{{Paris/Calendar_Links|July 21|July 23}}
{{Paris/Calendar_Links|July 21|July 23}}
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=='''Culture of Stable strain with biobricks 2008''' (in 6ml LB with adaptated antibiotics)==
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=='''Culture''' (in 6ml LB with adaptated antibiotics)==
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Culture will be use for Miniprep and Stock in glycerol, 2 clones isolated by Biobricks :
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Culture of '''Stable strain with biobricks 2008''':
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* will be use for Miniprep and Stock in glycerol
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* 2 clones isolated by Biobricks :
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|align="center"|'''Name'''
|align="center"|'''Name'''
|align="center"|'''Biobricks'''
|align="center"|'''Biobricks'''
 +
|align="center"|'''Description'''
|align="center"|'''Antibio'''
|align="center"|'''Antibio'''
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|align="center"|B0034
|align="center"|B0034
|align="center"|[[Image:Part_icon_rbs.png]]
|align="center"|[[Image:Part_icon_rbs.png]]
 +
|align="center"|RBS
|align="center"|A
|align="center"|A
|-
|-
|align="center"|C0040
|align="center"|C0040
|align="center"|[[Image:Icon_coding.png]]
|align="center"|[[Image:Icon_coding.png]]
 +
|align="center"|tetR repressor
|align="center"|A
|align="center"|A
|-
|-
|align="center"|C0079
|align="center"|C0079
|align="center"|[[Image:Icon_coding.png]]
|align="center"|[[Image:Icon_coding.png]]
 +
|align="center"|lasR activator
|align="center"|K
|align="center"|K
|-
|-
|align="center"|C0179  
|align="center"|C0179  
|align="center"|[[Image:Icon_coding.png]]
|align="center"|[[Image:Icon_coding.png]]
 +
|align="center"|lasR activator
|align="center"|A
|align="center"|A
|-
|-
|align="center"|E0030
|align="center"|E0030
-
|align="center"|[[Image:Part_icon_regulatory.png]]
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|align="center"|[[Image:Part_icon_reporter.png]]
 +
|align="center"|YFP
|align="center"|K
|align="center"|K
|-
|-
|align="center"|E0040
|align="center"|E0040
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center"|[[Image:Part_icon_reporter.png]]
 +
|align="center"|GFP
|align="center"|A
|align="center"|A
|-
|-
|align="center"|E1010
|align="center"|E1010
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center"|[[Image:Part_icon_reporter.png]]
 +
|align="center"|mRFP
|align="center"|K
|align="center"|K
|-
|-
|align="center"|J23101
|align="center"|J23101
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|[[Image:Part_icon_regulatory.png]]
 +
|align="center"|constitutive promoter member family
|align="center"|A
|align="center"|A
|-
|-
|align="center"|J23109
|align="center"|J23109
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|[[Image:Part_icon_regulatory.png]]
 +
|align="center"|constitutive promoter member family
|align="center"|A
|align="center"|A
|-
|-
|align="center"|R0040
|align="center"|R0040
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|[[Image:Part_icon_regulatory.png]]
 +
|align="center"|tetR repressible promoter
|align="center"|A
|align="center"|A
|-
|-
|align="center"|R0079
|align="center"|R0079
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|[[Image:Part_icon_regulatory.png]]
 +
|align="center"|promoter (lasr & PAI regulated)
|align="center"|A
|align="center"|A
|-
|-
|align="center"|pSB1A3
|align="center"|pSB1A3
|align="center"|[[Image:Icon_plasmid.png]]
|align="center"|[[Image:Icon_plasmid.png]]
 +
|align="center"|plasmid
|align="center"|A
|align="center"|A
|-
|-
|align="center"|SO3154
|align="center"|SO3154
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
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|align="center"|construction intermediate
|align="center"|A
|align="center"|A
|-
|-
|align="center"|S03879
|align="center"|S03879
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
 +
|align="center"|construction intermediate
|align="center"|A
|align="center"|A
|}
|}

Revision as of 15:11, 22 July 2008

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Culture (in 6ml LB with adaptated antibiotics)

Culture of Stable strain with biobricks 2008:

  • will be use for Miniprep and Stock in glycerol
  • 2 clones isolated by Biobricks :


Name Biobricks Description Antibio
B0034 Part icon rbs.png RBS A
C0040 Icon coding.png tetR repressor A
C0079 Icon coding.png lasR activator K
C0179 Icon coding.png lasR activator A
E0030 Part icon reporter.png YFP K
E0040 Part icon reporter.png GFP A
E1010 Part icon reporter.png mRFP K
J23101 Part icon regulatory.png constitutive promoter member family A
J23109 Part icon regulatory.png constitutive promoter member family A
R0040 Part icon regulatory.png tetR repressible promoter A
R0079 Part icon regulatory.png promoter (lasr & PAI regulated) A
pSB1A3 Icon plasmid.png plasmid A
SO3154 Part icon rbs.pngIcon coding.png construction intermediate A
S03879 Part icon rbs.pngIcon coding.png construction intermediate A