Team:Paris/July 26

From 2008.igem.org

(Difference between revisions)
(New page: {{Paris/Calendar_Links|July 25|July 27}} == MiniPreps == *The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th. {| border="1" |align="center"|'''...)
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|align="center"|MP100
|align="center"|MP100
-
|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
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|align="center"|
+
|align="center"|Strongest RBS (Efficiency = 1)
|-
|-
|align="center"|MP101
|align="center"|MP101
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101]
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|align="center"|
+
|align="center"|Strongest Constitutive promoter
|-
|-
|align="center"|MP102
|align="center"|MP102
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109]
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|align="center"|
+
|align="center"|Weak Constitutive promoter
|-
|-
|align="center"|MP103
|align="center"|MP103
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079]
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|align="center"|
+
|align="center"|promoter pLas (lasr & PAI regulated)
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|-
|align="center"|MP104
|align="center"|MP104
-
|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040]
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|align="center"|
+
|align="center"|tetR repressible promoter
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|align="center"|MP105
|align="center"|MP105
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154]
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|align="center"|
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|align="center"|B0034(rbs) ->LasI
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|-
|align="center"|MP106
|align="center"|MP106
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879]
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|align="center"|
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|align="center"|B0034(rbs) -> TetR
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|align="center"|MP107
|align="center"|MP107
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_C0079 C0079]
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|align="center"|
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|align="center"|lasR activator with LVA Tag
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|-
+
|-  
|align="center"|MP108
|align="center"|MP108
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_C0179 C0179]
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|align="center"|
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|align="center"|lasR activator
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|align="center"|MP109
|align="center"|MP109
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_E0030 E0030]
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|align="center"|
+
|align="center"|YFP
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|align="center"|MP110
|align="center"|MP110
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_E0040 E0040]
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|align="center"|
+
|align="center"|GFP
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|-
+
|-  
|align="center"|MP111
|align="center"|MP111
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|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_E1010 E1010]
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|align="center"|
+
|align="center"|mRFP
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|align="center"|MP116
|align="center"|MP116
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|align="center"|J23100
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|align="center"|[http://partsregistry.org/Part:BBa_J23100 J23100]
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|align="center"|
+
|align="center"|Strong constitutive promoter in J61002
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|align="center"|MP117
|align="center"|MP117
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|align="center"|J23107
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|align="center"|[http://partsregistry.org/Part:BBa_J23107 J23107]
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|align="center"|
+
|align="center"|Medium constitutive promoter in J61002
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|align="center"|MP118
|align="center"|MP118
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|align="center"|B0015
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|align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015]
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|align="center"|
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|align="center"|Double terminator
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|align="center"|MP119
|align="center"|MP119
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|align="center"|I0500
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|align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500]
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|align="center"|
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|align="center"|AraC pBAD
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|align="center"|MP120
|align="center"|MP120
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|align="center"|B0030
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|align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030]
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|align="center"|
+
|align="center"|Strong RBS (Efficiency = 0,6)
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|align="center"|MP121
|align="center"|MP121
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|align="center"|E0422
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|align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422]
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|align="center"|
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|align="center"|ECFP (RBS+LVA+Term)
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|-
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|align="center"|MP122
+
|align="center"| MP122
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|align="center"|E0840
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|align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840]
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|align="center"|
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|align="center"|gfp tri-part; strong rbs
 +
|
|}
|}
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== Digestion ==
== Digestion ==

Revision as of 14:40, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th.
Name Biobrick Description
MP100 B0034 Strongest RBS (Efficiency = 1)
MP101 J23101 Strongest Constitutive promoter
MP102 J23109 Weak Constitutive promoter
MP103 R0079 promoter pLas (lasr & PAI regulated)
MP104 R0040 tetR repressible promoter
MP105 S03154 B0034(rbs) ->LasI
MP106 S03879 B0034(rbs) -> TetR
MP107 C0079 lasR activator with LVA Tag
MP108 C0179 lasR activator
MP109 E0030 YFP
MP110 E0040 GFP
MP111 E1010 mRFP
MP116 J23100 Strong constitutive promoter in J61002
MP117 J23107 Medium constitutive promoter in J61002
MP118 B0015 Double terminator
MP119 I0500 AraC pBAD
MP120 B0030 Strong RBS (Efficiency = 0,6)
MP121 E0422 ECFP (RBS+LVA+Term)
MP122 E0840 gfp tri-part; strong rbs

Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3

Results of digestions : Electrophoresis

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