Team:Paris/July 26
From 2008.igem.org
(Difference between revisions)
(New page: {{Paris/Calendar_Links|July 25|July 27}} == MiniPreps == *The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th. {| border="1" |align="center"|'''...) |
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== MiniPreps == | == MiniPreps == | ||
- | * | + | |
+ | * Use of Promega's protocol on all the clones cultivated on the 25th. | ||
+ | * Preparation of 50µl of minipreps in double. | ||
+ | |||
{| border="1" | {| border="1" | ||
Line 12: | Line 15: | ||
|- | |- | ||
|align="center"|MP100 | |align="center"|MP100 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034] |
- | |align="center"| | + | |align="center"|Strongest RBS (Efficiency = 1) |
|- | |- | ||
|align="center"|MP101 | |align="center"|MP101 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101] |
- | |align="center"| | + | |align="center"|Strongest Constitutive promoter |
|- | |- | ||
|align="center"|MP102 | |align="center"|MP102 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109] |
- | |align="center"| | + | |align="center"|Weak Constitutive promoter |
|- | |- | ||
|align="center"|MP103 | |align="center"|MP103 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079] |
- | |align="center"| | + | |align="center"|promoter pLas (lasr & PAI regulated) |
|- | |- | ||
|align="center"|MP104 | |align="center"|MP104 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040] |
- | |align="center"| | + | |align="center"|tetR repressible promoter |
|- | |- | ||
|align="center"|MP105 | |align="center"|MP105 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154] |
- | |align="center"| | + | |align="center"|B0034(rbs) ->LasI |
|- | |- | ||
|align="center"|MP106 | |align="center"|MP106 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879] |
- | |align="center"| | + | |align="center"|B0034(rbs) -> TetR |
|- | |- | ||
|align="center"|MP107 | |align="center"|MP107 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_C0079 C0079] |
- | |align="center"| | + | |align="center"|lasR activator with LVA Tag |
- | |- | + | |- |
|align="center"|MP108 | |align="center"|MP108 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_C0179 C0179] |
- | |align="center"| | + | |align="center"|lasR activator |
|- | |- | ||
|align="center"|MP109 | |align="center"|MP109 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_E0030 E0030] |
- | |align="center"| | + | |align="center"|YFP |
|- | |- | ||
|align="center"|MP110 | |align="center"|MP110 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_E0040 E0040] |
- | |align="center"| | + | |align="center"|GFP |
- | |- | + | |- |
|align="center"|MP111 | |align="center"|MP111 | ||
- | |align="center"| | + | |align="center"|[http://partsregistry.org/Part:BBa_E1010 E1010] |
- | |align="center"| | + | |align="center"|mRFP |
|- | |- | ||
|align="center"|MP116 | |align="center"|MP116 | ||
- | |align="center"|J23100 | + | |align="center"|[http://partsregistry.org/Part:BBa_J23100 J23100] |
- | |align="center"| | + | |align="center"|Strong constitutive promoter in J61002 |
|- | |- | ||
|align="center"|MP117 | |align="center"|MP117 | ||
- | |align="center"|J23107 | + | |align="center"|[http://partsregistry.org/Part:BBa_J23107 J23107] |
- | |align="center"| | + | |align="center"|Medium constitutive promoter in J61002 |
|- | |- | ||
|align="center"|MP118 | |align="center"|MP118 | ||
- | |align="center"|B0015 | + | |align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] |
- | |align="center"| | + | |align="center"|Double terminator |
|- | |- | ||
|align="center"|MP119 | |align="center"|MP119 | ||
- | |align="center"|I0500 | + | |align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] |
- | |align="center"| | + | |align="center"|AraC pBAD |
|- | |- | ||
|align="center"|MP120 | |align="center"|MP120 | ||
- | |align="center"|B0030 | + | |align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] |
- | |align="center"| | + | |align="center"|Strong RBS (Efficiency = 0,6) |
|- | |- | ||
|align="center"|MP121 | |align="center"|MP121 | ||
- | |align="center"|E0422 | + | |align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] |
- | |align="center"| | + | |align="center"|ECFP (RBS+LVA+Term) |
|- | |- | ||
- | |align="center"|MP122 | + | |align="center"| MP122 |
- | |align="center"|E0840 | + | |align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] |
- | |align="center"| | + | |align="center"|gfp tri-part; strong rbs |
+ | | | ||
|} | |} | ||
- | |||
== Digestion == | == Digestion == | ||
- | |||
===Digestion Mix=== | ===Digestion Mix=== | ||
- | 10µl of Miniprep ( | + | 10µl of Miniprep (26 aug.) <br> |
12.5µl of water<br> | 12.5µl of water<br> | ||
2.5µl of Buffer N°2<br> | 2.5µl of Buffer N°2<br> | ||
Line 104: | Line 106: | ||
1µl of enzyme 2<br> | 1µl of enzyme 2<br> | ||
- | + | * Incubation 1h at 37°C with the first enzyme | |
+ | * Add the second enzyme | ||
+ | * Incubation 1h at 37°C with the second enzyme | ||
+ | * Store on ice | ||
+ | * Revelation of the digestion by electrophoresis on agarose gel | ||
- | {| border="1" | + | |
- | |align= | + | |
- | |align="center"|''' | + | === Results of digestions : Electrophoresis === |
- | |align="center"|''' | + | |
- | |align="center"|''' | + | '''conditions :''' |
- | |align="center"|''' | + | |
- | |align="center"|''' | + | * 10µl of ladder 1 kb (except for gel n°6 : 100 pb) |
+ | * 30µl of digestion added with 5µl of loading Dye 6x | ||
+ | * migration ~30min at 100W | ||
+ | * Gel 1, 2, 3, 4, 5 = '''0.8%''' | ||
+ | * Gel 6 = '''1,5%''' | ||
+ | |||
+ | |||
+ | gel1 [[Image:080726gel_1.jpg| gel1|200px]] | ||
+ | gel2 [[Image:080726gel_2.jpg| gel2|200px]] | ||
+ | gel3 [[Image:080726gel_3.jpg| gel3|200px]] | ||
+ | gel4 [[Image:080726gel_4.jpg| gel4|200px]]<br> | ||
+ | |||
+ | |||
+ | gel5 [[Image:080726gel_5.jpg| gel5|100px]] | ||
+ | gel6 [[Image:080726gel_6.jpg| gel6|100px]] | ||
+ | |||
+ | |||
+ | |||
+ | {|- border="1" | ||
+ | |align=center style="background: #649CD7;"|'''Name''' | ||
+ | |align=center style="background: #649CD7;"|'''BioBrick''' | ||
+ | |align=center style="background: #649CD7;"|'''Tube N°''' | ||
+ | |align=center style="background: #649CD7;"|'''Enz 1''' | ||
+ | |align=center style="background: #649CD7;"|'''Enz 2''' | ||
+ | |align=center style="background: #649CD7;"|'''Obs''' | ||
+ | |align=center style="background: #649CD7;"|'''Exp Size Matrix''' | ||
+ | |align=center style="background: #649CD7;"|'''Exp Size BB''' | ||
+ | |align=center style="background: #649CD7;"|'''Mea Size Matrix''' | ||
+ | |align=center style="background: #649CD7;"|'''Mea Size BB''' | ||
+ | |align=center style="background: #649CD7;"|'''Gel''' | ||
+ | |align=center style="background: #649CD7;"|'''Band''' | ||
|- | |- | ||
- | + | ! rowspan="2" style="background: #D4E2EF;" | D100 | |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | B0034 |
- | | | + | |align=center|1 |
- | | | + | ! rowspan="2"| XbaI |
- | | | + | ! rowspan="2"| PstI |
- | | | + | ! rowspan="2"| BI |
+ | ! rowspan="2"| 2057 pb | ||
+ | ! rowspan="2"| 34 pb | ||
+ | ! rowspan="2"| 1800 pb | ||
+ | ! rowspan="2"| 40 pb | ||
+ | ! rowspan="1"| 6 | ||
+ | ! rowspan="1"| 2 | ||
|- | |- | ||
- | |align= | + | |align=center|2 |
- | + | ! rowspan="1"| 6 | |
- | + | ! rowspan="1"| 3 | |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;" | D101 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;" | B0034 |
- | |align= | + | |align=center|3 |
- | |align= | + | |align=center| EcoRI |
- | |align= | + | |align=center| XbaI |
- | |align= | + | |align=center| FV |
+ | |align=center| 2076 pb | ||
+ | |align=center| 15 pb | ||
+ | |align=center| 2000 pb | ||
+ | |align=center| - | ||
+ | |align=center| 1 | ||
+ | |align=center| 2 | ||
|- | |- | ||
- | | | + | ! rowspan="2" style="background: #D4E2EF;" | D102 |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | B0034 |
- | | | + | |align=center|4 |
- | | | + | ! rowspan="2"| SpeI |
- | | | + | ! rowspan="2"| PstI |
- | |align= | + | ! rowspan="2"| BV |
+ | ! rowspan="2"| 2077 pb | ||
+ | ! rowspan="2"| 14 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| - | ||
+ | |align=center| 1 | ||
+ | |align=center| 3 | ||
|- | |- | ||
- | |align= | + | |align=center|5 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center| 4 |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | + | ! rowspan="2" style="background: #D4E2EF;" | D103 | |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | J23101 |
- | | | + | |align=center|1 |
- | | | + | ! rowspan="2"| SpeI |
- | | | + | ! rowspan="2"| PstI |
- | |align= | + | ! rowspan="2"| BV |
+ | ! rowspan="2"| 2100 pb | ||
+ | ! rowspan="2"| 883 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 700 pb | ||
+ | |align=center| 1 | ||
+ | |align=center| 5 | ||
|- | |- | ||
- | |align= | + | |align=center|2 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center| 6 |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | | | + | ! rowspan="2" style="background: #D4E2EF;" | D104 |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | J23109 |
- | | | + | |align=center|1 |
- | | | + | ! rowspan="2"| SpeI |
- | | | + | ! rowspan="2"| PstI |
- | | | + | ! rowspan="2"| BV |
+ | ! rowspan="2"| 2100 pb | ||
+ | ! rowspan="2"| 883 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 700 pb | ||
+ | ! rowspan="1"| 1 | ||
+ | ! rowspan="1"| 7 | ||
|- | |- | ||
- | |align= | + | |align=center|2 |
- | + | ! rowspan="1"| 1 | |
- | + | ! rowspan="1"| 8 | |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | | | + | ! rowspan="2" style="background: #D4E2EF;" | D105 |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | R0079 |
- | | | + | |align=center|1 |
- | | | + | ! rowspan="2"| SpeI |
- | | | + | ! rowspan="2"| PstI |
- | |align= | + | ! rowspan="2"| BV |
+ | ! rowspan="2"| 2222 pb | ||
+ | ! rowspan="2"| 14 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| - | ||
+ | |align=center| 1 | ||
+ | |align=center| 9 | ||
|- | |- | ||
- | |align= | + | |align=center|2 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center| 10 |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | | | + | ! rowspan="2" style="background: #D4E2EF;" | D106 |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | R0040 |
- | | | + | |align=center|1 |
- | | | + | ! rowspan="2"| SpeI |
- | | | + | ! rowspan="2"| PstI |
- | |align= | + | ! rowspan="2"| BV |
+ | ! rowspan="2"| 2119 pb | ||
+ | ! rowspan="2"| 14 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| - | ||
+ | |align=center| 1 | ||
+ | |align=center| 11 | ||
|- | |- | ||
- | |align= | + | |align=center|2 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center| 12 |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | + | ! rowspan="1" style="background: #D4E2EF;" | D107 | |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;" | S03154 |
- | | | + | |align=center|1 |
- | | | + | ! rowspan="1"| SpeI |
- | |align= | + | ! rowspan="1"| PstI |
- | |align= | + | ! rowspan="1"| BV |
+ | ! rowspan="1"| 2750 pb | ||
+ | ! rowspan="1"| 14 pb | ||
+ | |align=center| 3000 pb | ||
+ | |align=center| - | ||
+ | |align=center| 2 | ||
+ | |align=center| 5 | ||
|- | |- | ||
- | + | ! rowspan="2" style="background: #D4E2EF;" | D108 | |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | S03154 |
- | | | + | |align=center|2 |
- | | | + | ! rowspan="2"| XbaI |
- | | | + | ! rowspan="2"| PstI |
- | |align= | + | ! rowspan="2"| BI |
+ | ! rowspan="2"| 2057 pb | ||
+ | ! rowspan="2"| 707 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 700 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 2 | ||
|- | |- | ||
- | |align= | + | |align=center|3 |
- | |align= | + | |align=center| 2 |
- | |align= | + | |align=center| 3 |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | + | ! rowspan="1" style="background: #D4E2EF;" | D109 | |
- | + | ! rowspan="1" style="background: #D4E2EF;" | S03154 | |
- | |align= | + | |align=center|4 |
- | |align= | + | | align=center|EcoRI |
- | |align= | + | | align=center|SpeI |
- | |align= | + | | align=center|FI |
+ | | align=center| 2056 pb | ||
+ | | align=center| 708 pb | ||
+ | |align=center| 2000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 4 | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;" | D110 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;" | S03879 |
- | |align= | + | |align=center|1 |
- | |align= | + | | align=center|SpeI |
- | |align= | + | | align=center|PstI |
- | |align= | + | | align=center|BV |
+ | | align=center| 2768 pb | ||
+ | | align=center|14 pb | ||
+ | |align=center| 2700 pb | ||
+ | |align=center| - | ||
+ | |align=center| 2 | ||
+ | |align=center| 6 | ||
|- | |- | ||
- | | | + | ! rowspan="2" style="background: #D4E2EF;" | D111 |
- | |align= | + | ! rowspan="2" style="background: #D4E2EF;" | S03879 |
- | | | + | |align=center|2 |
- | | | + | ! rowspan="2"| XbaI |
- | | | + | ! rowspan="2"| PstI |
- | |align= | + | ! rowspan="2"| BI |
+ | ! rowspan="2"| 2057 pb | ||
+ | ! rowspan="2"| 725 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 700 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 7 | ||
|- | |- | ||
- | |align= | + | |align=center|3 |
- | |align= | + | |align=center| 2 |
- | |align= | + | |align=center| 8 |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;" | D112 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;" | S03879 |
- | |align= | + | |align=center|4 |
- | |align= | + | | align=center|EcoRI |
- | |align= | + | | align=center|SpeI |
- | |align= | + | | align=center|FI |
+ | | align=center| 2756 pb | ||
+ | | align=center| 726 pb | ||
+ | |align=center| 3000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 9 | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;" |D113 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;" |C0079 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center|EcoRI |
- | |align= | + | |align=center|SpeI |
- | |align= | + | |align=center|FI |
+ | |align=center| 4402 pb | ||
+ | |align=center| 779 pb | ||
+ | |align=center| 5000 pb | ||
+ | |align=center| 800 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 10 | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;" | D114 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;" | C0079 |
- | |align= | + | |align=center| 2 |
- | |align= | + | |align=center|XbaI |
- | |align= | + | |align=center|PstI |
- | |align= | + | |align=center|BI |
+ | |align=center| 4003 pb | ||
+ | |align=center| 778 pb | ||
+ | |align=center| 5000 pb | ||
+ | |align=center| 800 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 11 | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;"|D115 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;"|C0179 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center| EcoRI |
- | |align= | + | |align=center| SpeI |
- | |align= | + | |align=center| FI |
+ | |align=center| 4402 pb | ||
+ | |align=center| 746 pb | ||
+ | |align=center| 2000 pb | ||
+ | |align=center| 800 pb | ||
+ | |align=center| 2 | ||
+ | |align=center| 12 | ||
|- | |- | ||
- | + | ! rowspan="1" style="background: #D4E2EF;" | D116 | |
- | + | ! rowspan="1" style="background: #D4E2EF;" | C0179 | |
- | |align= | + | |align=center| 2 |
- | |align= | + | |align=center| XbaI |
- | |align= | + | |align=center| PstI |
- | |align= | + | |align=center| BI |
+ | |align=center| 4403 pb | ||
+ | |align=center| 745 pb | ||
+ | |align=center| 2000 pb | ||
+ | |align=center| 800 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 2 | ||
|- | |- | ||
- | | | + | ! rowspan="1" style="background: #D4E2EF;"|D117 |
- | |align= | + | ! rowspan="1" style="background: #D4E2EF;"|E0030 |
- | |align= | + | |align=center| 1 |
- | |align= | + | |align=center|EcoRI |
- | |align= | + | |align=center|SpeI |
- | |align= | + | |align=center|FI |
+ | |align=center| 3166 pb | ||
+ | |align=center| 746 pb | ||
+ | |align=center| 3000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 3 | ||
|- | |- | ||
- | |align="center"|D122 | + | ! rowspan="1" style="background: #D4E2EF;" | D118 |
- | |align="center"| | + | ! rowspan="1" style="background: #D4E2EF;" | E0030 |
- | |align="center"| | + | |align=center| 2 |
- | |align="center"|XbaI | + | |align=center| XbaI |
- | |align="center"|PstI | + | |align=center| PstI |
- | |align="center"|3 | + | |align=center| BI |
+ | |align=center| 3167 pb | ||
+ | |align=center| 745 pb | ||
+ | |align=center| 3000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 4 | ||
+ | |- | ||
+ | ! rowspan="1" style="background: #D4E2EF;"|D119 | ||
+ | ! rowspan="1" style="background: #D4E2EF;"|E0040 | ||
+ | |align=center| 1 | ||
+ | |align=center| EcoRI | ||
+ | |align=center| SpeI | ||
+ | |align=center| FI | ||
+ | |align=center| 2056 pb | ||
+ | |align=center| 743 pb | ||
+ | |align=center| 2000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 5 | ||
+ | |- | ||
+ | ! rowspan="1" style="background: #D4E2EF;" | D120 | ||
+ | ! rowspan="1" style="background: #D4E2EF;" | E0040 | ||
+ | |align=center| 2 | ||
+ | |align=center| XbaI | ||
+ | |align=center| PstI | ||
+ | |align=center| BI | ||
+ | |align=center| 2057 pb | ||
+ | |align=center| 742 pb | ||
+ | |align=center| 2000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 6 | ||
+ | |- | ||
+ | ! rowspan="1" style="background: #D4E2EF;"|D121 | ||
+ | ! rowspan="1" style="background: #D4E2EF;"|E1010 | ||
+ | |align=center| 1 | ||
+ | |align=center| EcoRI | ||
+ | |align=center| SpeI | ||
+ | |align=center| FI | ||
+ | |align=center| 4402 pb | ||
+ | |align=center| 704 pb | ||
+ | |align=center| 5000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 7 | ||
+ | |- | ||
+ | ! rowspan="1" style="background: #D4E2EF;" | D122 | ||
+ | ! rowspan="1" style="background: #D4E2EF;" | E1010 | ||
+ | |align=center| 2 | ||
+ | |align=center| XbaI | ||
+ | |align=center| PstI | ||
+ | |align=center| BI | ||
+ | |align=center| 4403 pb | ||
+ | |align=center| 703 pb | ||
+ | |align=center| 5000 pb | ||
+ | |align=center| 700 pb | ||
+ | |align=center| 3 | ||
+ | |align=center| 8 | ||
+ | |- | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|D123 | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|J23100 | ||
+ | |align=center|1 | ||
+ | ! rowspan="2"| SpeI | ||
+ | ! rowspan="2"| PstI | ||
+ | ! rowspan="2"| BV | ||
+ | ! rowspan="2"| 2100 pb | ||
+ | ! rowspan="2"| 883 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 900 pb | ||
+ | ! rowspan="1"| 3 | ||
+ | ! rowspan="1"| 9 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="1"| 3 | ||
+ | ! rowspan="1"| 10 | ||
+ | |- | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|D124 | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|J23107 | ||
+ | |align=center|1 | ||
+ | ! rowspan="2"| SpeI | ||
+ | ! rowspan="2"| PstI | ||
+ | ! rowspan="2"| BV | ||
+ | ! rowspan="2"| 2100 pb | ||
+ | ! rowspan="2"| 883 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 900 pb | ||
+ | ! rowspan="1"| 3 | ||
+ | ! rowspan="1"| 11 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="1"| 3 | ||
+ | ! rowspan="1"| 12 | ||
+ | |- | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|D125 | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|B0015 | ||
+ | |align=center|1 | ||
+ | ! rowspan="2"|EcoRI | ||
+ | ! rowspan="2"|XbaI | ||
+ | ! rowspan="2"| BV | ||
+ | ! rowspan="2"| 3303 pb | ||
+ | ! rowspan="2"| 15 pb | ||
+ | ! rowspan="2"| 3000 pb | ||
+ | ! rowspan="2"| - | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 2 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 3 | ||
+ | |- | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|D126 | ||
+ | ! rowspan="2" style="background: #D4E2EF;"|I0500 | ||
+ | |align=center|1 | ||
+ | ! rowspan="2"|SpeI | ||
+ | ! rowspan="2"|PstI | ||
+ | ! rowspan="2"| FV | ||
+ | ! rowspan="2"| 5621 pb | ||
+ | ! rowspan="2"| 14 pb | ||
+ | ! rowspan="2"| 6000 pb | ||
+ | ! rowspan="2"| - | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 4 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 5 | ||
+ | |- | ||
+ | ! rowspan="2" style="background: #D4E2EF;" | D127 | ||
+ | ! rowspan="2" style="background: #D4E2EF;" | B0030 | ||
+ | |align=center|1 | ||
+ | ! rowspan="2"| XbaI | ||
+ | ! rowspan="2"| PstI | ||
+ | ! rowspan="2"| BI | ||
+ | ! rowspan="2"| 2057 pb | ||
+ | ! rowspan="2"| 37 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 50 pb | ||
+ | ! rowspan="1"| 6 | ||
+ | ! rowspan="1"| 4 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="1"| 6 | ||
+ | ! rowspan="1"| 5 | ||
+ | |- | ||
+ | ! rowspan="1" style="background: #D4E2EF;" | D128 | ||
+ | ! rowspan="1" style="background: #D4E2EF;" | B0030 | ||
+ | |align=center|3 | ||
+ | ! rowspan="1"| EcoRI | ||
+ | ! rowspan="1"| XbaI | ||
+ | ! rowspan="1"| FV | ||
+ | ! rowspan="1"| 2079 pb | ||
+ | ! rowspan="1"| 15 pb | ||
+ | ! rowspan="1"| 2200 pb | ||
+ | ! rowspan="1"| - | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 6 | ||
+ | |- | ||
+ | ! rowspan="2" style="background: #D4E2EF;" | D129 | ||
+ | ! rowspan="2" style="background: #D4E2EF;" | B0030 | ||
+ | |align=center|4 | ||
+ | ! rowspan="2"| SpeI | ||
+ | ! rowspan="2"| PstI | ||
+ | ! rowspan="2"| BV | ||
+ | ! rowspan="2"| 2080 pb | ||
+ | ! rowspan="2"| 14 pb | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| - | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 7 | ||
+ | |- | ||
+ | |align=center|5 | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 8 | ||
+ | |- | ||
+ | ! rowspan="3" style="background: #D4E2EF;"|D130 | ||
+ | ! rowspan="3" style="background: #D4E2EF;"|E0422 | ||
+ | |align=center|1 | ||
+ | ! rowspan="3"| XbaI | ||
+ | ! rowspan="3"| PstI | ||
+ | ! rowspan="3"| BI | ||
+ | ! rowspan="3"| 2057 pb | ||
+ | ! rowspan="3"| 939 pb | ||
+ | ! rowspan="3"| 2000 pb | ||
+ | ! rowspan="3"| 1100 pb | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 9 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 10 | ||
+ | |- | ||
+ | |align=center|3 | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 11 | ||
+ | |- | ||
+ | ! rowspan="3" style="background: #D4E2EF;"|D131 | ||
+ | ! rowspan="3" style="background: #D4E2EF;"|E0840 | ||
+ | |align=center|1 | ||
+ | ! rowspan="3"| XbaI | ||
+ | ! rowspan="3"| PstI | ||
+ | ! rowspan="3"| BI | ||
+ | ! rowspan="3"| 2057 pb | ||
+ | ! rowspan="3"| 900 pb | ||
+ | ! rowspan="1"| 2000 pb | ||
+ | ! rowspan="1"| 1000 pb | ||
+ | ! rowspan="1"| 4 | ||
+ | ! rowspan="1"| 12 | ||
+ | |- | ||
+ | |align=center|2 | ||
+ | ! rowspan="2"| 2000 pb | ||
+ | ! rowspan="2"| 900 pb | ||
+ | ! rowspan="1"| 5 | ||
+ | ! rowspan="1"| 2 | ||
+ | |- | ||
+ | |align=center|3 | ||
+ | ! rowspan="1"| 5 | ||
+ | ! rowspan="1"| 3 | ||
|} | |} | ||
- | == | + | |
+ | ==> conclusion : all the digestion have succeed.....GREAT ! | ||
+ | |||
+ | == DNA extraction == | ||
+ | |||
+ | |||
+ | * Cutting of the parts of interest, for all the digestion that have migrated on the gels | ||
+ | * Use of Promega's protocol for the extraction. | ||
+ | * Test of the succeed of the extraction by electrophoresis on 2µl of the parts extracted. | ||
+ | |||
+ | |||
+ | ==> conclusion : we succeed to detect DNA in our samples this time. |
Latest revision as of 16:55, 31 July 2008
MiniPreps
DigestionDigestion Mix10µl of Miniprep (26 aug.)
Results of digestions : Electrophoresisconditions :
DNA extraction
|