Team:Paris/July 26

Revision as of 14:49, 26 July 2008 by Fanny.c (Talk | contribs)

MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th.

Name	Biobrick	Description
MP100	B0034	Strongest RBS (Efficiency = 1)
MP101	J23101	Strongest Constitutive promoter
MP102	J23109	Weak Constitutive promoter
MP103	R0079	promoter pLas (lasr & PAI regulated)
MP104	R0040	tetR repressible promoter
MP105	S03154	B0034(rbs) ->LasI
MP106	S03879	B0034(rbs) -> TetR
MP107	C0079	lasR activator with LVA Tag
MP108	C0179	lasR activator
MP109	E0030	YFP
MP110	E0040	GFP
MP111	E1010	mRFP
MP116	J23100	Strong constitutive promoter in J61002
MP117	J23107	Medium constitutive promoter in J61002
MP118	B0015	Double terminator
MP119	I0500	AraC pBAD
MP120	B0030	Strong RBS (Efficiency = 0,6)
MP121	E0422	ECFP (RBS+LVA+Term)
MP122	E0840	gfp tri-part; strong rbs

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2