Team:Paris/July 26

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th.
Name Biobrick Description
MP100 B0034 Strongest RBS (Efficiency = 1)
MP101 J23101 Strongest Constitutive promoter
MP102 J23109 Weak Constitutive promoter
MP103 R0079 promoter pLas (lasr & PAI regulated)
MP104 R0040 tetR repressible promoter
MP105 S03154 B0034(rbs) ->LasI
MP106 S03879 B0034(rbs) -> TetR
MP107 C0079 lasR activator with LVA Tag
MP108 C0179 lasR activator
MP109 E0030 YFP
MP110 E0040 GFP
MP111 E1010 mRFP
MP116 J23100 Strong constitutive promoter in J61002
MP117 J23107 Medium constitutive promoter in J61002
MP118 B0015 Double terminator
MP119 I0500 AraC pBAD
MP120 B0030 Strong RBS (Efficiency = 0,6)
MP121 E0422 ECFP (RBS+LVA+Term)
MP122 E0840 gfp tri-part; strong rbs


Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2


Results of digestions : Electrophoresis

gel1 [[Image:| gel1|200px]] gel2 [[Image:| gel2|200px]] gel3 [[Image:| gel3|200px]] gel4 [[Image:| gel4|200px]]


gel5 [[Image:‎| gel5|200px]] gel6 [[Image:‎| gel6|200px]]



Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb 6 2
2 6 3
3 EcoRI XbaI FV 2076 pb 15 pb 1 2
4 SpeI PstI BV 2077 pb 14 pb 1 3
5 1 4
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb 1 5
2 1 6
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb 1 7-8
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb 1 9
2 1 10
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb 1 11
2 1 12
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb 2 5
2 XbaI PstI BI 2057 pb 707 pb 2 2
3 2 3
4 EcoRI SpeI FI 2056 pb 708 pb 2 4
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb 2 6
2 XbaI PstI BI 2057 pb 725 pb 2 7
3 2 8
4 EcoRI SpeI FI 2756 pb 726 pb 2 9
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb 2 10
2 XbaI PstI BI 4003 pb 778 pb 2 11
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb 2 12
2 XbaI PstI BI 4403 pb 745 pb 3 2
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb 3 3
2 XbaI PstI BI 3167 pb 745 pb 3 4
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb 3 5
2 XbaI PstI BI 2057 pb 742 pb 3 6
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb 3 7
2 XbaI PstI BI 4403 pb 703 pb 3 8
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb 3 9
2 3 10
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb 3 11
2 3 12
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb 4 2
2 4 3
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb 4 4
2 4 5
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb 6 4
2 6 5
3 EcoRI XbaI FV 2079 pb 15 pb 4 6
4 SpeI PstI BV 2080 pb 14 pb 4 7
5 4 8
D121 E0422 1 XbaI PstI BI 2057 pb 939 pb 4 9
2 4 10
3 4 11
D122 E0840 1 XbaI PstI BI 2057 pb 900 pb
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