Team:Paris/July 28

From 2008.igem.org

(Difference between revisions)
(→Results of the extraction : Electrophoresis)
(→Results of the extraction : Electrophoresis)
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! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 34 pb
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! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2077 pb
! rowspan="2"| 2077 pb
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! rowspan="2"| 2000 pb
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|align=center| 1 & 7
|align=center| 1 & 7
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! rowspan="2"| BV
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! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
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! rowspan="2"| 2000 pb
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! rowspan="1"| -
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! rowspan="1"| 10
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Revision as of 11:56, 30 July 2008

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Extraction of the DNA

Results of the extraction : Electrophoresis

conditions :

  • 10µl of ladder 1 kb
  • xµl of extraction added with 2µl of loading Dye 6x
  • migration ~30min at 100W


  • Gel 1, 2, 3, 4 = 0,8%
  • 2µl of extraction added with 2µl of loading Dye 6x
  • Gel 5, 6 = 0,8%
  • 3µl of extraction added with 2µl of loading Dye 6x
  • Gel 7 = 0,8%
  • 5µl of extraction added with 2µl of loading Dye 6x
  • Gel 8 = 1,5%
  • 10µl of extraction added with 2µl of loading Dye 6x


gel1 gel1 gel2 gel2 gel3 gel3 gel4 gel4 gel5 gel5


gel6 gel6 gel7 gel7 gel8 gel8



Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Mea Size Conc (ng/µl) Gel Band
D100 B0034 1 XbaI PstI BI 34 pb - 0 8 5
2 - - -
D101 B0034 3 EcoRI XbaI FV 2076 pb - - 1 & 7 2 & 2
D102 B0034 4 SpeI PstI BV 2077 pb 2000 pb 10 1 & 7 3 & 3
5 - 1 & 7 4 & 4
D103 J23101 1 SpeI PstI BV 2100 pb 2000 pb 10 1 5
2 - 1 6
D104 J23109 1 SpeI PstI BV 2100 pb 1 7
2 1 8
D105 R0079 1 SpeI PstI BV 2222 pb 2 2
2 2 3
D106 R0040 1 SpeI PstI BV 2119 pb 2 & 7 4 & 5
2 2 & 7 5 & 6
D107 S03154 1 SpeI PstI BV 2750 pb 2 & 7 6 & 7
D108 S03154 2 XbaI PstI BI 707 pb 2 & 7 7 & 8
3 2 & 7 8 & 9
D109 S03154 4 EcoRI SpeI FI 708 pb 3 & 7 2 & 10
D110 S03879 1 SpeI PstI BV 2768 pb 3 3
D111 S03879 2 XbaI PstI BI 725 pb 3 4
3 3 5
D112 S03879 4 EcoRI SpeI FI 726 pb 3 6
D113 C0079 1 EcoRI SpeI FI 779 pb 3 7
D114 C0079 2 XbaI PstI BI 778 pb 3 8
D115 C0179 1 EcoRI SpeI FI 746 pb 4 & 7 2 & 11
D116 C0179 2 XbaI PstI BI 745 pb 4 & 7 3 & 12
D117 E0030 1 EcoRI SpeI FI 746 pb 4 4
D118 E0030 2 XbaI PstI BI 745 pb 4 5
D119 E0040 1 EcoRI SpeI FI 743 pb 4 & 7 6 & 13
D120 E0040 2 XbaI PstI BI 742 pb 4 & 7 7 & 14
D121 E1010 1 EcoRI SpeI FI 704 pb 4 8
D122 E1010 2 XbaI PstI BI 703 pb 5 2
D123 J23100 1 SpeI PstI BV 2100 pb 5 3
2 5 4
D124 J23107 1 SpeI PstI BV 2100 pb 5 5
2 5 6
D124 J23107 1 EcoRI XbaI FV 3303 pb 5 7
2 5 8
D126 I0500 1 SpeI PstI FV 5621 pb 5 9
2 5 10
D127 B0030 1 XbaI PstI BI 37 pb 8 3
2 - - -
D128 B0030 3 EcoRI XbaI FV 2079 pb 5 11
D129 B0030 4 SpeI PstI BV 2080 pb 5 12
5 5 13
D130 E0422 1 XbaI PstI BI 939 pb 5 14
2 5 15
3 5 16
D131 E0840 1 XbaI PstI BI 900 pb 6 3
2 6 4
3 6 5


==> conclusion :

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