Team:Paris/July 28

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Revision as of 17:44, 30 July 2008

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Extraction of the DNA

Results of the extraction : Electrophoresis

conditions :

10µl of ladder 1 kb
xµl of extraction added with 2µl of loading Dye 6x
migration ~30min at 100W

Gel 1, 2, 3, 4 = 0,8%
2µl of extraction added with 2µl of loading Dye 6x

Gel 5, 6 = 0,8%
3µl of extraction added with 2µl of loading Dye 6x

Gel 7 = 0,8%
5µl of extraction added with 2µl of loading Dye 6x

Gel 8 = 1,5%
10µl of extraction added with 2µl of loading Dye 6x

gel1 gel2 gel3 gel4 gel5

gel6 gel7 gel8

Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size	Mea Size	Conc (ng/µl)	Gel	Band
D100	B0034	1	XbaI	PstI	BI	34 pb	-	0	8	5
D100	B0034	2	XbaI	PstI	BI	34 pb	-	-	-
D101	B0034	3	EcoRI	XbaI	FV	2076 pb	-	-	1 & 7	2 & 2
D102	B0034	4	SpeI	PstI	BV	2077 pb	2000 pb	10	1 & 7	3 & 3
D102	B0034	5	SpeI	PstI	BV	2077 pb	2000 pb	-	1 & 7	4 & 4
D103	J23101	1	SpeI	PstI	BV	2100 pb	2000 pb	-	1	5
D103	J23101	2	SpeI	PstI	BV	2100 pb	2000 pb	10	1	6
D104	J23109	1	SpeI	PstI	BV	2100 pb	2000 pb	20	1	7
D104	J23109	2	SpeI	PstI	BV	2100 pb	2000 pb	20	1	8
D105	R0079	1	SpeI	PstI	BV	2222 pb	2000 pb	5	2	2
D105	R0079	2	SpeI	PstI	BV	2222 pb	2000 pb	5	2	3
D106	R0040	1	SpeI	PstI	BV	2119 pb	-	-	2 & 7	4 & 5
D106	R0040	2	SpeI	PstI	BV	2119 pb	-	-	2 & 7	5 & 6
D107	S03154	1	SpeI	PstI	BV	2750 pb	-	-	2 & 7	6 & 7
D108	S03154	2	XbaI	PstI	BI	707 pb	-	-	2 & 7	7 & 8
D108	S03154	3	XbaI	PstI	BI	707 pb	-	-	2 & 7	8 & 9
D109	S03154	4	EcoRI	SpeI	FI	708 pb	-	-	3 & 7	2 & 10
D110	S03879	1	SpeI	PstI	BV	2768 pb	3000 pb	20	3	3
D111	S03879	2	XbaI	PstI	BI	725 pb	-	-	3	4
D111	S03879	3	XbaI	PstI	BI	725 pb	-	-	3	5
D112	S03879	4	EcoRI	SpeI	FI	726 pb	-	-	3	6
D113	C0079	1	EcoRI	SpeI	FI	779 pb	800 pb	20	3	7
D114	C0079	2	XbaI	PstI	BI	778 pb	800 pb	20	3	8
D115	C0179	1	EcoRI	SpeI	FI	746 pb	-	-	4 & 7	2 & 11
D116	C0179	2	XbaI	PstI	BI	745 pb	-	-	4 & 7	3 & 12
D117	E0030	1	EcoRI	SpeI	FI	746 pb	700 pb	5	4	4
D118	E0030	2	XbaI	PstI	BI	745 pb	700 pb	5	4	5
D119	E0040	1	EcoRI	SpeI	FI	743 pb	-	-	4 & 7	6 & 13
D120	E0040	2	XbaI	PstI	BI	742 pb	-	-	4 & 7	7 & 14
D121	E1010	1	EcoRI	SpeI	FI	704 pb	700 pb	5	4	8
D122	E1010	2	XbaI	PstI	BI	703 pb	800 pb	10	5	2
D123	J23100	1	SpeI	PstI	BV	2100 pb	2000 pb	10	5	3
D123	J23100	2	SpeI	PstI	BV	2100 pb	2000 pb	10	5	4
D124	J23107	1	SpeI	PstI	BV	2100 pb	2000 pb	10	5	5
D124	J23107	2	SpeI	PstI	BV	2100 pb	2000 pb	15	5	6
D124	J23107	1	EcoRI	XbaI	FV	3303 pb	3000 pb	15	5	7
D124	J23107	2	EcoRI	XbaI	FV	3303 pb	3000 pb	15	5	8
D126	I0500	1	SpeI	PstI	FV	5621 pb	6000 pb	10	5	9
D126	I0500	2	SpeI	PstI	FV	5621 pb	6000 pb	10	5	10
D127	B0030	1	XbaI	PstI	BI	37 pb	-	-	8	3
D127	B0030	2	XbaI	PstI	BI	37 pb	-	-	-	-
D128	B0030	3	EcoRI	XbaI	FV	2079 pb	2000 pb	10	5	11
D129	B0030	4	SpeI	PstI	BV	2080 pb	2000 pb	10	5	12
D129	B0030	5	SpeI	PstI	BV	2080 pb	2000 pb	10	5	13
D130	E0422	1	XbaI	PstI	BI	939 pb	1100 pb	10	5	14
		2						10	5	15
		3						10	5	16
D131	E0840	1	XbaI	PstI	BI	900 pb	1000 pb	5	6	3
		2						10	6	4
		3						5	6	5

==> conclusion :

@@ Line 107: / Line 107: @@
 ! rowspan="2"| 2100 pb
 ! rowspan="2"| 2000 pb
-! rowspan="1"| 10
+! rowspan="1"| -
 |align=center| 1
 |align=center| 5
 |-
 |align=center|2
-|align=center| -
+|align=center| 10
 |align=center| 1
 ! rowspan="1"| 6
@@ Line 123: / Line 123: @@
 ! rowspan="2"| BV
 ! rowspan="2"| 2100 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="1"|
+! rowspan="1"| 20
 ! rowspan="1"| 1
 ! rowspan="1"| 7
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 20
 ! rowspan="1"| 1
 ! rowspan="1"| 8
@@ Line 140: / Line 140: @@
 ! rowspan="2"| BV
 ! rowspan="2"| 2222 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-|align=center|
+|align=center| 5
 ! rowspan="1"| 2
 |align=center| 2
 |-
 |align=center|2
-|align=center|
+|align=center| 5
 |align=center| 2
 ! rowspan="1"| 3
@@ Line 157: / Line 157: @@
 ! rowspan="2"| BV
 ! rowspan="2"| 2119 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center|-
 ! rowspan="1"| 2 & 7
 |align=center| 4 & 5
 |-
 |align=center|2
-|align=center|
+|align=center| -
 |align=center| 2 & 7
 ! rowspan="1"| 5 & 6
@@ Line 174: / Line 174: @@
 ! rowspan="1"| BV
 ! rowspan="1"| 2750 pb
-|align=center|
+|align=center|-
-! rowspan="1"|
+! rowspan="1"| -
 |align=center| 2 & 7
 |align=center| 6 & 7
@@ Line 186: / Line 186: @@
 ! rowspan="2"| BI
 ! rowspan="2"| 707 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center| -
 |align=center| 2 & 7
 ! rowspan="1"| 7 & 8
 |-
 |align=center|3
-|align=center|
+|align=center| -
 |align=center| 2 & 7
 ! rowspan="1"| 8 & 9
@@ Line 203: / Line 203: @@
 |align=center|FI
 |align=center| 708 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| -
 ! rowspan="1"| 3 & 7
 |align=center| 2 & 10
@@ Line 215: / Line 215: @@
 |align=center|BV
 |align=center| 2768 pb
-|align=center|
+|align=center| 3000 pb
-|align=center|
+|align=center| 20
 |align=center| 3
 ! rowspan="1"| 3
@@ Line 227: / Line 227: @@
 ! rowspan="2"| BI
 ! rowspan="2"| 725 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center| -
 |align=center| 3
 ! rowspan="1"| 4
 |-
 |align=center|3
-|align=center|
+|align=center| -
 |align=center| 3
 ! rowspan="1"| 5
@@ Line 244: / Line 244: @@
 |align=center|FI
 |align=center| 726 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| -
 ! rowspan="1"| 3
 |align=center| 6
@@ Line 256: / Line 256: @@
 |align=center|FI
 |align=center| 779 pb
-|align=center|
+|align=center| 800 pb
-|align=center|
+|align=center| 20
 ! rowspan="1"| 3
 |align=center| 7
@@ Line 268: / Line 268: @@
 |align=center|BI
 |align=center| 778 pb
-|align=center|
+|align=center| 800 pb
-|align=center|
+|align=center| 20
 |align=center| 3
 ! rowspan="1"| 8
@@ Line 280: / Line 280: @@
 |align=center| FI
 |align=center| 746 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| -
 |align=center| 4 & 7
 ! rowspan="1"| 2 & 11
@@ Line 292: / Line 292: @@
 |align=center| BI
 |align=center| 745 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| -
 ! rowspan="1"| 4 & 7
 |align=center| 3 & 12
@@ Line 304: / Line 304: @@
 |align=center|FI
 |align=center| 746 pb
-|align=center|
+|align=center| 700 pb
-|align=center|
+|align=center| 5
 |align=center| 4
 ! rowspan="1"| 4
@@ Line 316: / Line 316: @@
 |align=center| BI
 |align=center| 745 pb
-|align=center|
+|align=center| 700 pb
-|align=center|
+|align=center| 5
 ! rowspan="1"| 4
 |align=center| 5
@@ Line 328: / Line 328: @@
 |align=center| FI
 |align=center| 743 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| -
 ! rowspan="1"| 4 & 7
 |align=center| 6 & 13
@@ Line 340: / Line 340: @@
 |align=center| BI
 |align=center| 742 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| -
 ! rowspan="1"| 4 & 7
 |align=center| 7 & 14
@@ Line 352: / Line 352: @@
 |align=center| FI
 |align=center| 704 pb
-|align=center|
+|align=center| 700 pb
-|align=center|
+|align=center| 5
 |align=center| 4
 ! rowspan="1"| 8
@@ Line 365: / Line 365: @@
 |align=center| BI
 |align=center| 703 pb
-|align=center|
+|align=center| 800 pb
-|align=center|
+|align=center| 10
 |align=center| 5
 ! rowspan="1"| 2
@@ Line 377: / Line 377: @@
 ! rowspan="2"| BV
 ! rowspan="2"| 2100 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 3
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 4
@@ Line 394: / Line 394: @@
 ! rowspan="2"| BV
 ! rowspan="2"| 2100 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 5
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 15
 ! rowspan="1"| 5
 ! rowspan="1"| 6
@@ Line 411: / Line 411: @@
 ! rowspan="2"| FV
 ! rowspan="2"| 3303 pb
-! rowspan="2"|
+! rowspan="2"| 3000 pb
-! rowspan="1"|
+! rowspan="1"| 15
 ! rowspan="1"| 5
 ! rowspan="1"| 7
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 15
 ! rowspan="1"| 5
 ! rowspan="1"| 8
@@ Line 428: / Line 428: @@
 ! rowspan="2"| FV
 ! rowspan="2"| 5621 pb
-! rowspan="2"|
+! rowspan="2"| 6000 pb
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 9
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 10
@@ Line 445: / Line 445: @@
 ! rowspan="2"| BI
 ! rowspan="2"| 37 pb
-! rowspan="2"|
+! rowspan="2"| -
-! rowspan="1"|
+! rowspan="1"| -
 ! rowspan="1"| 8
 ! rowspan="1"| 3
@@ Line 462: / Line 462: @@
 ! rowspan="1"|  FV
 ! rowspan="1"| 2079 pb
-! rowspan="1"|
+! rowspan="1"| 2000 pb
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 11
@@ Line 474: / Line 474: @@
 ! rowspan="2"| BV
 ! rowspan="2"| 2080 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 12
 |-
 |align=center|5
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 13
@@ Line 491: / Line 491: @@
 ! rowspan="3"| BI
 ! rowspan="3"| 939 pb
-! rowspan="3"|
+! rowspan="3"| 1100 pb
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 14
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 15
 |-
 |align=center|3
-! rowspan="1"|
+! rowspan="1"| 10
 ! rowspan="1"| 5
 ! rowspan="1"| 16
@@ Line 513: / Line 513: @@
 ! rowspan="3"| BI
 ! rowspan="3"| 900 pb
-! rowspan="1"|
+! rowspan="3"| 1000 pb
-! rowspan="1"|
+! rowspan="1"| 5
 ! rowspan="1"| 6
 ! rowspan="1"| 3
 |-
 |align=center|2
-! rowspan="1"|
+! rowspan="1"| 10
-! rowspan="1"|
 ! rowspan="1"| 6
 ! rowspan="1"| 4
 |-
 |align=center|3
-! rowspan="1"|
+! rowspan="1"| 5
-! rowspan="1"|
 ! rowspan="1"| 6
 ! rowspan="1"| 5