Team:Paris/July 30

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Revision as of 16:52, 4 August 2008

Analysis of yesterday DNA digestion

The digested DNA of yesterday was analysed one more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.

D101	B0034	1	EcoRI	XbaI	FV	2076 bp	2000 bp	6	10
Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size	Mea Size	Conc (ng/µl)	Band
D102	B0034	1	SpeI	PstI	BV	2077 bp	2000 bp	8	11
D105	R0079	1	SpeI	PstI	BV	2222 bp	2000 bp	6	13
D106	R0040	1	SpeI	PstI	BV	2119 bp	2000 bp	6	12
D108	S03154	1	XbaI	PstI	BI	707 bp		0 (failed)	14
D111	S03879	1	XbaI	PstI	BI	725 bp	600 bp	2	15
D115	C0179	2	EcoRI	SpeI	FI	746 pb		0 (failed)	4 & 5
D116	C0179	2	XbaI	PstI	BI	745 pb			2 & 3
D117	E0030	1	EcoRI	SpeI	FI	746 pb			16
D128	B0030	2	EcoRI	XbaI	FV	2079 pb			6 & 7
D129	B0030	2	SpeI	PstI	BV	2080 pb			8 & 9

Results : Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).

@@ Line 28: / Line 28: @@
 ! rowspan="1"| XbaI
 ! rowspan="1"| FV
-! rowspan="1"| 2076 pb
+! rowspan="1"| 2076 bp
-! rowspan="1"| 2000 pb
+! rowspan="1"| 2000 bp
-! rowspan="1"|
+! rowspan="1"| 6
 ! rowspan="1"| 10
 |-
@@ Line 39: / Line 39: @@
 ! rowspan="1"| PstI
 ! rowspan="1"| BV
-! rowspan="1"| 2077 pb
+! rowspan="1"| 2077 bp
-! rowspan="1"|
+! rowspan="1"| 2000 bp
-! rowspan="1"|
+! rowspan="1"| 8
 ! rowspan="1"| 11
 |-
@@ Line 50: / Line 50: @@
 ! rowspan="1"| PstI
 ! rowspan="1"| BV
-! rowspan="1"| 2222 pb
+! rowspan="1"| 2222 bp
-! rowspan="1"|
+! rowspan="1"| 2000 bp
-! rowspan="1"|
+! rowspan="1"| 6
 ! rowspan="1"| 13
 |-
@@ Line 61: / Line 61: @@
 ! rowspan="1"| PstI
 ! rowspan="1"| BV
-! rowspan="1"| 2119 pb
+! rowspan="1"| 2119 bp
-! rowspan="1"|
+! rowspan="1"| 2000 bp
-! rowspan="1"|
+! rowspan="1"| 6
 ! rowspan="1"| 12
 |-
@@ Line 72: / Line 72: @@
 ! rowspan="1"| PstI
 ! rowspan="1"| BI
-! rowspan="1"| 707 pb
+! rowspan="1"| 707 bp
 ! rowspan="1"|
 ! rowspan="1"| 0 (failed)
@@ Line 83: / Line 83: @@
 ! rowspan="1"| PstI
 ! rowspan="1"| BI
-! rowspan="1"| 725 pb
+! rowspan="1"| 725 bp
-! rowspan="1"|
+! rowspan="1"| 600 bp
-! rowspan="1"|
+! rowspan="1"| 2
 ! rowspan="1"| 15
 |-