Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)
(Transformation results)
(Results of Ligation transformant)
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|align="center"|a lot ++  
|align="center"|a lot ++  
|align="center"|strong constitutive promoter <-> ECFP
|align="center"|strong constitutive promoter <-> ECFP
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|align="center"|5+8
 
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|align="center"|L106
|align="center"|L106

Revision as of 17:12, 4 August 2008

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Results of Ligation transformant

Ligation name Nb of colonies Description
L100 0 rbs-TetR <-> ECFP (RBS+LVA+Term)
L101 (1) rbs-TetR <-> GFP tripart+strong rbs
L102 +/- 100 strong RBS (0,6)<-> YFP
L103 a lot ++ strong RBS (0,6)<-> GFP
L104 a lot ++ strong RBS (0,6)<-> LasR activator (+LVA)
L105 a lot ++ strong constitutive promoter <-> ECFP
L106 a lot ++ strong constitutive promoter <-> GFP tripart
L107 a lot ++ strongest constitutive promoter <-> ECFP
L108 +/- 100 (2 dishes!?) strongest constitutive promoter <-> GFP tripart
L109 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP
L110 a lot ++ medium constitutive promoter <-> gfp tripart
L111 a lot ++ weak constitutive promoter <-> ECFP
L112 a lot ++ weak constitutive promoter <-> gfp tripart
L113 1 Arac pBAD <-> ECFP
L114 2 Arac pBAD <-> gfp tripart
L115 a lot ++ pLas <-> ECFP
L116 a lot ++ pLas <-> gfp tripart
L117 +/- 30 Double terminator <-> YFP
L118 +/- 30 Double terminator <-> mRFP
L119 +/- 30 Double terminator <-> LasR activator (+LVA)
L120 a lot ++ tetR repressible promoter <-> ECFP
L121 a lot ++ tetR repressible promoter <-> gfp tripart
L122 0 B0034rbs-LasI <-> ECFP
L123 1 B0034rbs-LasI <-> gfp tripart
L124 a lot ++ strongest RBS (1) <-> GFP
L125 a lot ++ strongest RBS (1) <-> YFP
L126 no dish found!!! strongest RBS (1) <-> activator (+LVA)
L127 no dish found!!! Double terminator <-> GFP
C1 0 D110
C2 5 D129
C3 +/- 100 D123
C4 +/- 20 D103
C5 +/- 100 Double terminator <-> GFP
C6 +/- 100 Double terminator <-> GFP
C7 0 Double terminator <-> GFP
C8 +/- 20 Double terminator <-> GFP
C9 0 Double terminator <-> GFP
C10 0 Double terminator <-> GFP
C11 0 Double terminator <-> GFP
C12 +/- 10 Double terminator <-> GFP
Positive control 155 (transformation efficiency:1.5*10^7/ug) -