Team:Paris/July 31

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Results of the transformation : Fluorescence

Ligation name Description Fluorescence Comments
L102

Strong rbs - YFP
D129 (BV) - D118 (BI)

No OK
L103

Strong rbs - GFP
D129 (BV) - D122 (BI)

No OK
L104

Strong rbs - lasR activator
D129 (BV) - D114 (BI)

No OK
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

Yes OK (some others red because of the promoter)
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

Yes (some others red because of the promoter) OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

Yes (some others red because of the promoter) OK
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

Yes (some others red because of the promoter) OK
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

Yes (some others red because of the promoter) OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

No To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

No To do again
L115

pLas - ECFP
D105 (BV)- D130 (BI)

No OK
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

1 green To do again
L117

yfp - Double Terminator
D117 (FI) - D125 (FV)

No OK
L118

rfp - Double Terminator
D121 (FI) - D125 (FV)

No OK
L119

lasR activator + LVA - Double Terminator
D113 (FI) - D125 (FV)

No OK
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

No To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

Yes (some others red because of the promoter) OK
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

No OK
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

No OK