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Revision as of 14:13, 30 July 2008

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock) PCR products PCR products Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Biobricks Description Products in use Date MP100 B0034 Strongest RBS (Efficiency = 1) July 26 MP101 J23101 Strongest Constitutive promoter July 22July 23July 26 MP102 J23109 Weak Constitutive promoter July 22July 26 MP103 R0079 promoter pLas (lasr & PAI regulated) July 23July 26 MP104 R0040 tetR repressible promoter July 23July 26 MP105 S03154 B0034(rbs) ->LasI July 26 MP106 S03879 B0034(rbs) -> TetR July 26 MP107 C0079 lasR activator with LVA Tag July 23 MP108 C0179 lasR activator July 22July 23July 26 MP109 E0030 YFP July 26 MP110 E0040 GFP July 23July 26 MP111 E1010 mRFP July 23July 26 MP112 OmpR M571 July 23 MP113 EnvZ I2815 July 23 MP114 C0040 tetR repressor July 23 MP115 pSB1A3 July 23 MP116 pJ23100 in J61002 Strong constitutive promoter in J61002 July 26 MP117 pJ23107 in J61002 Medium constitutive promoter in J61002 July 24July 26 MP118 B0015 in pSB1AK3 Double terminator July 24July 26 MP119 I0500 in pSB2K3 AraC pBAD July 24July 26 MP120 B0030 in pSB1A2 Strong RBS (Efficiency = 0,6) July 26 MP121 E0422 in pSB1A2 ECFP (RBS+LVA+Term) July 24July 26 MP122 E0840 in pSB1A2 gfp tri-part; strong rbs July 26 Digestion Products (Legend: Vector, Insert) Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product Left []ng/µL Date D100.1 B0034 pSB1A2 XbaI PstI BI 2057 34 10 July 26 D100.2 B0034 pSB1A2 XbaI PstI BI 2057 34 10 July 26 D101 B0034 pSB1A2 EcoRI XbaI FV 2076 15 10 July 29 D102.1 B0034 pSB1A2 SpeI PstI BV 2077 14 10 July 26 D102.2 B0034 pSB1A2 SpeI PstI BV 2077 14 10 July 29 D103.1 J23101 pSB1A2 SpeI PstI BV 2100 883 10 July 26 D103.2 J23101 pSB1A2 SpeI PstI BV 2100 883 10 July 26 D104.1 J23109 J61002 SpeI PstI BV 2100 883 20 July 26 D104.2 J23109 J61002 SpeI PstI BV 2100 883 20 July 26 D105.1 R0079 pSB1A2 SpeI PstI BV 2222 14 5 July 26 D105.2 R0079 pSB1A2 SpeI PstI BV 2222 14 5 July 29 D106.1 R0040 pSB1A2 SpeI PstI BV 2119 14 8 July 26 D106.2 R0040 pSB1A2 SpeI PstI BV 2119 14 5 July 29 D107 S03154 pSB1A2 SpeI PstI BV 2750 14 5 July 26 D108.1 S03154 pSB1A2 XbaI PstI BI 2057 707 5 July 26 D108.2 S03154 pSB1A2 XbaI PstI BI 2057 707 5 July 29 D109 S03154 pSB1A2 EcoRI SpeI FI 2056 708 5 July 26 D110 S03879 pSB1A2 SpeI PstI BV 2768 14 20 July 26 D111.1 S03879 pSB1A2 XbaI PstI BI 2057 725 20 July 26 D111.2 S03879 pSB1A2 XbaI PstI BI 2057 725 20 July 29 D112 S03879 pSB1A2 EcoRI SpeI FI 2756 726 20 July 26 D113 C0079 pSB2K3 EcoRI SpeI FI 4402 779 20 July 26 D114 C0079 pSB2K3 XbaI PstI BI 4003 778 20 July 26 D115.1 C0179 pSB1A2 EcoRI SpeI FI 4402 746 - July 29 D115.2 C0179 pSB1A2 EcoRI SpeI FI 4402 746 - July 29 D116.1 C0079 pSB1A2 XbaI PstI BI 4403 745 20 July 29 D116.1 C0079 pSB1A2 XbaI PstI BI 4403 745 20 July 29 D117 E0030 pSB1AK3 EcoRI SpeI FI 3166 746 5 July 29 D118 E0030 pSB2K3 XbaI PstI BI 3167 745 20 July 26 D119 E0040 pSB1A2 EcoRI SpeI FI 2056 743 10 July 26 D120 E0040 pSB1A2 XbaI PstI BI 2057 742 10 July 26 D121 E1010 pSB2K3 EcoRI SpeI FI 4402 704 10 July 26 D122 E0040 pSB1A2 XbaI PstI BI 4403 703 10 July 26 D123.1 J23100 J61002 SpeI PstI BV 2100 883 10 July 26 D123.2 J23100 J61002 SpeI PstI BV 2100 883 10 July 26 D124.1 J23107 J61002 SpeI PstI BV 2100 883 15 July 26 D124.2 J23107 J61002 SpeI PstI BV 2100 883 15 July 26 D125.1 B0015 pSB1AK3 EcoRI XbaI FV 3303 15 15 July 26 D125.2 B0015 pSB1AK3 EcoRI XbaI FV 3303 15 15 July 26 D126.1 I0500 pSB2K3 SpeI PstI BV 5621 14 10 July 26 D126.2 I0500 pSB2K3 SpeI PstI BV 5621 14 10 July 26 D127.1 B0030 pSB1A2 XbaI PstI BI 2057 37 10 July 26 D127.2 B0030 pSB1A2 XbaI PstI BI 2057 37 10 July 26 D128.1 B0030 pSB1A2 EcoRI XbaI FV 2079 15 10 July 29 D128.1 B0030 pSB1A2 EcoRI XbaI FV 2079 15 10 July 29 D129.1 B0030 pSB1A2 SpeI PstI BV 2080 14 10 July 29 D129.2 B0030 pSB1A2 SpeI PstI BV 2080 14 10 July 29 D130.1 E0422 pSB1A2 XbaI PstI BI 2057 939 10 July 26 D130.2 E0422 pSB1A2 XbaI PstI BI 2057 939 10 July 26 D130.3 E0422 pSB1A2 XbaI PstI BI 2057 939 10 July 26 D131.1 E0840 pSB1A2 XbaI PstI BI 2057 900 10 July 26 D131.2 E0840 pSB1A2 XbaI PstI BI 2057 900 10 July 26 D131.3 E0840 pSB1A2 XbaI PstI BI 2057 900 10 July 26 Strains (glycerol stock) Strains glycerol stock Number Name Description Verified clone Date S103 I0500 AraC pBAD July 23 S106 E0422 ECFP (RBS+LVA+Term) July 23 S107 B0034 Strongest RBS (Efficiency = 1) July 23 S108 R0079 promoter pLas (lasr & PAI regulated) July 23 S109 J23101 Strongest Constitutive promoter July 23 S110 J23109 Weak Constitutive promoter July 23 S111 R0040 tetR repressible promoter July 23 S112 S03154 B0034(rbs) ->LasI July 23 S113 S03879 B0034(rbs) -> TetR July 23 S114 C0079 lasR activator with LVA Tag July 23 S115 C0179 lasR activator July 23 S116 E0030 YFP July 23 S117 E0040 GFP July 23 S118 E1010 mRFP July 23 S119 OmpR M571 July 23 S120 EnvZ I2815 July 23 S121 C0040 tetR repressor July 23 S122 pSB1A3 July 23

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