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From 2008.igem.org

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock) PCR products PCR products Number Name Oligo F Oligo R Template Length Comments Product Left ? []µg/µL Date PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7 PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7 PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7 PCR127 flhDC O131 O132 MG1655 no August 8 PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8 PCR129 EnvZ O126 O127 Strain EnvZ* 1400pb yes 100 August 8 PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11 PCR131 flhD rbs- O132 O133 MG1655 350pb no August 11 PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11 PCR133 flhDC + prom O110 O131 MG1655 1300pb no August 11 PCR134 flhDC + prom O111 O131 MG1655 no August 11 PCR135 pfliL O124 O125 MG1655 yes August 11 PCR136 pFlhDC O110 O113 MG1655 yes August 11 PCR137 pFlhDC O111 O113 MG1655 yes August 11 Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Biobricks Description Products in use Date MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 26 MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 26 July 22, July 23, July 26 MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Weak Constitutive promoter July 26 July 22, July 26 MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 26 July 23, July 26 MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 26 July 23, July 26 MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI July 26 MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR July 26 MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 26 July 22, July 23, July 26 MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 26 MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 26 July 23, July 26 MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 26 July 23, July 26 MP112 OmpR M571 July 23 MP113 EnvZ I2815 July 23 MP114 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor July 23 MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 July 26 MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 July 26 July 24, July 26 MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Double terminator July 26 July 24, July 26 MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] AraC pBAD July 26 July 24, July 26 MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong RBS (Efficiency = 0,6) July 26 MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] ECFP (RBS+LVA+Term) July 26 July 24, July 26 MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp tri-part; strong rbs July 26 MP123 [http://partsregistry.org/Part:J61002 J61002] August 5 MP124 L102 Strong rbs - YFP D129 (BV) - D118 (BI) August 1 MP125 L103 Strong rbs - mRFP D129 (BV) - D122 (BI) August 1 MP126 L104 Strong rbs - lasR activator D129 (BV) - D114 (BI) August 1 MP127 L105 Strong promoter - ECFP D123 (BV) - D130 (BI) August 1 MP128 L106 Strong promoter - gfp Tripart D123 (BV) - D131 (BI) August 1 MP129 L107 Strong promoter - ECFP D103 (BV) - D130 (BI) August 1 MP130 L108 Strong promoter - gfp Tripart D103 (BV) - D131 (BI) August 1 MP131 L110 Medium promoter - gfp Tripart D124 (BV) - D131 (BI) August 1 MP132 L111 Weak promoter - ECFP D104 (BV) - D130 (BI) August 1 MP133 L112 Weak promoter - GFP tripart D104 (BV) - D131 (BI) August 1 MP134 L115 pLas - ECFP D105 (BV) - D130 (BI) August 1 MP135 L116 pLas - gfp Tripart D105 (BV) - D131 (BI) August 1 MP136 L117 yfp - Double Terminator D125 (FV) - D117 (FI) August 1 MP137 L118 rfp - Double Terminator D125 (FV) - D121 (FI) August 1 MP138 L119 lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI) August 1 MP139 L121 pTet - gfp tripart D106 (BV) - D131 (BI) August 1 MP140 L124 Strongest RBS - rfp D102 (BV) - D122 (BI) August 1 MP141 L125 Strongest RBS - yfp D102 (BV) - D118 (BI) August 1 MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] August 8 MP143 [http://partsregistry.org/Part:E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator in pSB1A2 August 8 MP144 L128 pFlgA-RFP D132 (FI) - D136 (FV) MP144.3 ; MP144.4 August 12 MP145 L129 pFlgB-RFP D133 (FI) - D136 (FV) MP145.6 ; MP145.7 August 12 MP146 L130 pFlhB-RFP D134 (FI) - D136 (FV) MP146.1 ; MP146.2 August 12 MP147 L100 rbs TetR - ECFP D110 (BV) - D130 (BI) August 13 MP148 L101 rbs TetR - GFP tripart D110 (BV) - D131 (BI) August 13 MP149 L114 AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13 MP150 L120 tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13 MP151 L122 RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13 MP152 L123 RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13 MP153 L126 Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 13 MP154 L132 pSB1A2 - flhDC D142(FV) - D139(FI) August 13 MP155 L133 pSB1A2 - OmpR* D142(FV) - D140(FI) August 13 MP156 L134 pSB1A2 - EnvZ* D142(FV) - D141(FI) August 13 MP157 L138 pSB3K3 - gfp generator (E0240) D137(FV) - D138(FI) August 13 Digestion Products (Legend: Vector, Insert) Ø Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product Left []ng/µL Date D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D100.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29 D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26 D102.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 29 D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26 D103.2 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26 D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D104.2 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26 D105.2 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 29 D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26 D106.2 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 5 July 29 D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 5 July 26 D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 26 D108.2 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29 D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 5 July 26 D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 20 July 26 D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26 D111.2 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 29 D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2756 726 20 July 26 D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26 D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26 D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D115.2 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29 D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26 D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26 D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26 D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26 D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26 D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26 D123.2 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26 D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D124.2 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 15 July 26 D125.2 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 15 July 26 D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D126.2 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D127.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D129.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.2 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.3 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D131.2 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D131.3 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D132 pFlgA - EcoRI SpeI FI - 250 5 August 6 D133 pFlgB - EcoRI SpeI FI - 250 5 August 6 D134 pFlhB - EcoRI SpeI FI - 249 5 August 6 D135 pFlhDC - EcoRI SpeI FI - problem August 6 D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6 D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6 D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6 D139 flhDC - EcoRI PstI FI - 972 August 6 D140 E040 rbs+ - PstI PstI I - August 12 D141 flhD RBS - - EcoRI SpeI FI - August 12 D142 flhC RBS - - EcoRI SpeI FI - August 12 D143 flhDC + promoter - EcoRI SpeI FI - August 12 D144 pSBK3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] PstI PstI BI - August 12 D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcpRI SpeI BI - August 12 Strains (glycerol stock) Strains glycerol stock Number Name Biobricks Description Verified clone Date S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD July 23 S106 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+Term July 23 S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 23 S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 23 S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 23 S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Weak Constitutive promoter July 23 S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 23 S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI July 23 S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR July 23 S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 23 S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 23 S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 23 S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 23 S119 OmpR M571 July 23 S120 EnvZ I2815 July 23 S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor July 23 S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 S123 L102 Strong rbs - YFP D129 (BV) - D118 (BI) Aug 1 S124 L103 Strong rbs - mRFP D129 (BV) - D122 (BI) Aug 1 S125 L104 Strong rbs - lasR activator D129 (BV) - D114 (BI) Aug 1 S126 L105 Strong promoter - ECFP D123 (BV) - D130 (BI) Aug 1 S127 L106 Strong promoter - gfp Tripart D123 (BV) - D131 (BI) Aug 1 S128 L107 Strongest promoter - ECFP D103 (BV) - D130 (BI) Aug 1 S129 L108 Strong promoter - GFP tripart D103 (BV) - D131 (BI) Aug 1 S130 L110 Medium promoter - gfp tripart D124 (BV) - D131 (BI) Aug 1 S131 L111 Weak promoter - ECFP D104 (BV) - D130 (BI) Aug 1 S132 L112 Weak promoter - gfp Tripart D104 (BV) - D131 (BI) Aug 1 S133 L115 pLas - ECFP D105 (BV) - D130 (BI) Aug 1 S134 L116 pLas - gfp Tripart D105 (BV) - D131 (BI) Aug 1 S135 L117 yfp - Double Terminator D125 (FV) - D117 (FI) Aug 1 S136 L118 rfp - Double Terminator D125 (FV) - D121 (FI) Aug 1 S137 L119 LasR activator + LVA - Double terminator D125 (FV) - D113 (FI) Aug 1 S138 L121 pTet - gfp Tripart D106 (BV) - D131 (BI) Aug 1 S139 L124 Strongest rbs - mRFP D102 (BV) - D122 (BI) Aug 1 S140 L125 Strongest rbs - yfp D102 (BV) - D118 (BI) Aug 1

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