Team:Paris/Notebook/Freezer

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Revision as of 17:35, 11 September 2008 by Hs00590 (Talk | contribs)

Contents

Last ligation number

Last Ligation number used
185

Last digestion number

Last digestion number used

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 flhDC O132 O131 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 flhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 flhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 flhDC + prom O111 O131 MG1655 no August 11
PCR135 pfliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O 111 O 113 MG1655 yes August 11
PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15
PCR139 pflhDC (URI) O 110 O 113 PCR 130 yes August 15
PCR140 flhDC (RBS -) O 132 O 131 PCR 130 yes August 15
PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26
PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26
PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26
PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26
PCR145 PCRFliA1 O 148 O 152 MG1655 150pb yes August 26
PCR146 PCRFliA2 O 153 O 150 MG1655 670pb yes August 26
PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26
PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26
PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26
PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26
PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26
PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number Name Plamsid Biobricks Description Products in use/comments Conc. ug/mL Date
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 26
MP101.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 26 333 July 22, July 23, July 26
MP101.2 finished
MP101.4 200
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 pJ61002] Part icon regulatory.png Weak Constitutive promoter July 26 July 22, July 26
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 26 July 23, July 26
MP104.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 26 145 July 23, July 26
MP104.3 147
MP104.4 51
MP105.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI 34 July 26
MP105.2 31
MP106.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR 80 August 24
MP106.3 40
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png lasR activator July 26 July 22, July 23, July 26
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.png YFP July 26
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.png GFP July 26 July 23, July 26
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon reporter.png mRFP July 26 July 23, July 26
MP112 OmpR M57I Icon coding.png July 23
MP113 EnvZ I281S Icon coding.png July 23
MP114.1 [http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png tetR repressor 173 July 23
MP114.2 263
MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 July 26
MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 July 26 July 24, July 26
MP118.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator July 26 220 July 24, July 26
MP118.3 187
MP119.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 26 42 July 24, July 26
MP119.2 26
MP119.3 39
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strong RBS (Efficiency = 0,6) July 26
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png ECFP (RBS+LVA+Double Term) July 26 July 24, July 26
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png gfp tri-part; strong rbs July 26
MP123 [http://partsregistry.org/Part:J61002 J61002] Icon plasmid.png August 5
MP124 L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - YFP
D129 (BV) - D118 (BI)
August 1
MP125 L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP
D129 (BV) - D122 (BI)
August 1
MP126 L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strong rbs - lasR activator
D129 (BV) - D114 (BI)
August 1
MP127 L105 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP
D123 (BV) - D130 (BI)
August 1
MP128 L106 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)
August 1
MP129 L107 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP
D103 (BV) - D130 (BI)
August 1
MP130 L108 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart
D103 (BV) - D131 (BI)
August 1
MP131 L110 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)
August 1
MP132 L111 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - ECFP
D104 (BV) - D130 (BI)
August 1
MP133 L112 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - GFP tripart
D104 (BV) - D131 (BI)
August 1
MP134 L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - ECFP
D105 (BV) - D130 (BI)
August 1
MP135 L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - gfp Tripart
D105 (BV) - D131 (BI)
August 1
MP136 L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator
D125 (FV) - D117 (FI)
August 1
MP137 L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator
D125 (FV) - D121 (FI)
August 1
MP138 L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon coding.pngPart icon terminator.pngPart icon terminator.png lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI)
August 1
MP139 L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pTet - gfp tripart
D106 (BV) - D131 (BI)
August 1
MP140 L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - rfp
D102 (BV) - D122 (BI)
August 1
MP141 L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - yfp
D102 (BV) - D118 (BI)
August 1
MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon plasmid.png August 8
MP143.1 [http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator 138 August 8
MP143.2 150
MP144.1 L128 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgA-RFP
D132 (FI) - D136 (FV)
MP144.3 ; MP144.4 127 August 12
MP144.2 166
MP144.3 163
MP144.4 154
MP145.1 L129 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgB-RFP
D133 (FI) - D136 (FV)
MP145.6 ; MP145.7 136 August 12
MP145.2 151
MP145.6 163
MP145.7 188
MP146.1 L130 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlhB-RFP
D134 (FI) - D136 (FV)
MP146.1 ; MP146.2 298 August 12
MP146.2 142
MP146.7 170
MP146.8 143
MP147 L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - ECFP
D110 (BV) - D130 (BI)
August 13
MP148.1 L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - GFP tripart
D110 (BV) - D131 (BI) DO NOT WORK !!!!
MP148.2 ; MP148.3 109 August 13
MP148.2 123
MP148.3 123
MP149 L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png AracpBAD - gfp tripart
D126 (BV) - D131 (BI)
August 13
MP150 L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)
August 13
MP151 L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS-lasI - ECFP
D107 (BV) - D130 (BI)
August 13
MP152 L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS lasI - gfp tripart
D107 (BV) - D131 (BI)
August 13
MP153.1 L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
MP153.1 ; MP153.3 100 August 13
MP153.2 131
MP153.3 113
MP154 L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhDC
D142(FV) - D139(FI)
August 13
MP155 L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR*
D142(FV) - D140(FI)
WT sequence (August 19) August 13 ; August 19
MP156 L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ*
D142(FV) - D141(FI)
lacks about 300 bp in EnvZ* (August 19) August 13 August 19
MP157 L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator (E0240)
D137(FV) - D138(FI)
August 13
MP158 L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+
D140 (PstI) - D144 (ES)
seems to have the plasmid without the insert and one with the insert August 15
MP159 L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD
D142 (ES) - D145 (ES)
has not been sequenced August 15
MP160 L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor
D143 (ES) - D145 (ES)
does not have the insert August 15
MP161 L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png pLas + rbs-tetR-gfp tripart
D105 (SP) - D146 (XP)
incorrect clone -> to do again August 19
MP162.1 L151.1 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-lasR + double terminator
D147 (ES) - D125 (EX)
151 August 19
MP162.2 172
MP162.3 149
MP163.1 [http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.png RBS 80 August 20
MP163.2 79
MP164 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ ECFP+ LVA+ term August 20
MP165 [http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ YFP+ LVA- term August 20
MP166 [http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry August 20
MP167 [http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ ECFP LVA- term ceci ne contient pas de LVA apparemment. ça correspondrait au ECFP normal de longue durée de vie August 20
MP168 [http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ mRFP LVA+ term August 20
MP171 L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
gfp generator(E0240) - pTet August 25
MP172 L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png
RBS(B0032) - tetR August 25

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product in use []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
August 15
D102.2
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26
D103.2 35
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5
12
July 26
July 23
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D108.2
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20
8
19
July 26
August 23
August 28
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26
D123.2 23
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26
10
July 26
August 23
August 28
D125.2 24
3687
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2
D130.3
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26
D131.2
D131.3
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
August 16
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
August 15
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png
August 15
D149 pfliL - EcoRI SpeI FI - August 14
August 16
D150 pfliL - XbaI PstI BI - August 14
August 16
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14
D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15
D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15
D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15
D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16
D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16
D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14
11
August 16
August 23
D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV - 3 August 16
D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator
D147 (FI) - D125 (FV)
Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
27 August 18
D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 bp RBS (B0034)+ YFP+ LVA- term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png

59
August 26
September 3
D179 MP3.4 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012)
Part icon terminator.pngPart icon terminator.png
August 20
D180 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV promoter J23101
Part icon regulatory.png
August 20
D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter)
Part icon regulatory.png
August 20
D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR
Icon coding.png
August 20
D183 MP119.3 - XbaI PstI BI pBad promoter
Part icon regulatory.png
August 20
D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator
Part icon reporter.png
August 18
D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS
Part icon rbs.png
August 18
D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png

43

September 3
D188 PCR 138 - XbaI PstI Insert - 767 bp OmpR*
Icon coding.png
August 26
D189 MP 101.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] XbaI PstI Vector - 2073 bp pSB1A2
Icon plasmid.png
August 26
D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR
Icon coding.png
22 August 29
D192 MP144.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI XbaI FV 2267 FlgA
Part icon regulatory.png
26 August 29
D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
9,5 August 29
D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png
13 August 29
D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png
11 August 29
D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet
Part icon regulatory.png
45 September 3
D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
12 September 3
D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
36 September 3
D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
61 September 3
D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
43 September 3
D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR
Icon coding.png
13 August 29
D202 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI nothing 3037 25 September 8

Strains (glycerol stock)

Strains glycerol stock
Number Name Biobricks Description Verified clone Date
S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 23
S106 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png ECFP (RBS+LVA+ Double Term) July 23
S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 23
S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 23
S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 23
S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Weak Constitutive promoter July 23
S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 23
S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI July 23
S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR July 23
S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png lasR activator July 23
S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.png YFP July 23
S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.png GFP July 23
S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon reporter.png mRFP July 23
S119 OmpR M57I Icon coding.png July 23
S120 EnvZ I281S Icon coding.png July 23
S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png tetR repressor July 23
S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
S123 L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - YFP
D129 (BV) - D118 (BI)
Aug 1
S124 L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP
D129 (BV) - D122 (BI)
Aug 1
S125 L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strong rbs - lasR activator
D129 (BV) - D114 (BI)
Aug 1
S126 L105 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP
D123 (BV) - D130 (BI)
Aug 1
S127 L106 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)
Aug 1
S128 L107 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strongest promoter - ECFP
D103 (BV) - D130 (BI)
Aug 1
S129 L108 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - GFP tripart
D103 (BV) - D131 (BI)
Aug 1
S130 L110 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Medium promoter - gfp tripart
D124 (BV) - D131 (BI)
Aug 1
S131 L111 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - ECFP
D104 (BV) - D130 (BI)
Aug 1
S132 L112 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - gfp Tripart
D104 (BV) - D131 (BI)
Aug 1
S133 L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - ECFP
D105 (BV) - D130 (BI)
Aug 1
S134 L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - gfp Tripart
D105 (BV) - D131 (BI)
Aug 1
S135 L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator
D125 (FV) - D117 (FI)
Aug 1
S136 L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator
D125 (FV) - D121 (FI)
Aug 1
S137 L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon coding.pngPart icon terminator.pngPart icon terminator.png LasR activator + LVA - Double terminator
D125 (FV) - D113 (FI)
Aug 1
S138 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pTet - gfp Tripart
D106 (BV) - D131 (BI)
Aug 1
S139 L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest rbs - mRFP
D102 (BV) - D122 (BI)
Aug 1
S140 L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest rbs - yfp
D102 (BV) - D118 (BI)
Aug 1
S141 MG1655 Aug 7
S142 [http://partsregistry.org/Part:J61002 J61002] Icon plasmid.png Aug 7
S143 L128 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgA-RFP
D132 (FI) - D136 (FV)
MP144.3 ; MP144.4 August 12
S144 L129 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgB-RFP
D133 (FI) - D136 (FV)
MP145.6 ; MP145.7 August 12
S145 L130 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlhB-RFP
D134 (FI) - D136 (FV)
MP146.1 ; MP146.2 August 12
S146 L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - ECFP
D110 (BV) - D130 (BI)
August 13
S147 L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
August 13
S148 L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png AracpBAD - gfp tripart
D126 (BV) - D131 (BI)
August 13
S149 L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)
August 13
S150 L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS-lasI - ECFP
D107 (BV) - D130 (BI)
August 13
S151 L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS lasI - gfp tripart
D107 (BV) - D131 (BI)
August 13
S152 L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
August 13
S153 L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhDC
D142(FV) - D139(FI)
August 13
S154 L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR*
D142(FV) - D140(FI)
August 13
S154 L133.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR*
D142(FV) - D140(FI)
WT sequence August 19
S155 L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ*
D142(FV) - D141(FI)
August 13
S155 L134.2 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ*
D142(FV) - D141(FI)
lacks about 300 bp in EnvZ* August 19
S156 L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator (E0240)
D137(FV) - D138(FI)
August 13
S157 E0240 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png GFP generator August 15
S158 pSB3K3 Icon plasmid.png plasmid August 15
S159.1 L139.1 in in [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png E0240 RBS+
D140 (PstI) - D144 (ES)
seems to have the plasmid without the insert and one with the insert August 15
S159.2 incorrect clone
S160.1 L141.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhD
D142 (ES) - D145 (ES)
has not been sequenced August 15
S160.2 has not been sequenced
S160.3 has not been sequenced
S161.1 L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.png flhDC+promotor
D143 (ES) - D145 (ES)
does not seem to have the insert August 15
S161.2 incorrect clone
S162 MP162.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-lasR + double terminator
D147 (ES) - D125 (EX)
August 18
S163 B0032 in pSB1A2 Icon rbs.png medium rbs (efficiency = 0,3) August 20
S164 E0422 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs ECFP LVA+ double terminator August 20
S165 E0430 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs YFP LVA- double terminator August 20
S166 E0432 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs YFP LVA+ double terminator August 20
S167 E0420 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
strongest rbs ECFP LVA- double terminator August 20
S168 I732078 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs mRFP LVA+ double terminator August 20