Plasmids: MiniPrep Products 2008
|
Number
| Name
| Plamsid
| Biobricks
| Description
| Products in use/comments
| Conc. ug/mL
| Date
|
MP100
| B0034
| pSB1A2
|
| Strongest RBS (Efficiency = 1)
|
|
| July 26
|
MP101.1
| J23101
| J61002
|
| Strongest Constitutive promoter
| July 26
| 333
| July 22, July 23, July 26
|
MP101.2
| finished
|
MP101.4
| 200
|
MP102
| J23109
| J61002
|
| Weak Constitutive promoter
| July 26
|
| July 22, July 26
|
MP103
| R0079
| pSB1A2
|
| promoter pLas (lasr & PAI regulated)
| July 26
|
| July 23, July 26
|
MP104.1
| R0040
| pSB1A2
|
| tetR repressible promoter
| July 26
| 145
| July 23, July 26
|
MP104.3
| 147
|
MP104.4
| 51
|
MP105.1
| S03154
| pSB1A2
|
| B0034(rbs) ->LasI
|
| 34
| July 26
|
MP105.2
|
| 31
|
MP106.1
| S03879
| pSB1A2
|
| B0034(rbs) -> TetR
|
| 80
| August 24
|
MP106.3
|
| 40
|
MP107
| C0079
| pSB2K3
|
| lasR activator with LVA Tag
|
|
| July 23
|
MP108
| C0179
| pSB1A2
|
| lasR activator
| July 26
|
| July 22, July 23, July 26
|
MP109
| E0030
| pSB1AK3
|
| YFP
|
|
| July 26
|
MP110
| E0040
| pSB1A2
|
| GFP
| July 26
|
| July 23, July 26
|
MP111
| E1010
| pSB2K3
|
| mRFP
| July 26
|
| July 23, July 26
|
MP112
| OmpR M57I
|
|
|
|
|
| July 23
|
MP113
| EnvZ I281S
|
|
|
|
|
| July 23
|
MP114.1
| C0040
| pSB1A2
|
| tetR repressor
|
| 173
| July 23
|
MP114.2
|
| 263
|
MP115
| pSB1A3
|
|
|
|
|
| July 23
|
MP116
| J23100
| J61002
|
| Strong constitutive promoter in J61002
|
|
| July 26
|
MP117
| J23107
| J61002
|
| Medium constitutive promoter in J61002
| July 26
|
| July 24, July 26
|
MP118.1
| B0015
| pSB1AK3
|
| Double terminator
| July 26
| 220
| July 24, July 26
|
MP118.3
| 187
|
MP119.1
| I0500
| pSB2K3
|
| AraC pBAD
| July 26
| 42
| July 24, July 26
|
MP119.2
| 26
|
MP119.3
| 39
|
MP120
| B0030
| pSB1A2
|
| Strong RBS (Efficiency = 0,6)
|
|
| July 26
|
MP121
| E0422
| pSB1A2
|
| ECFP (RBS+LVA+Double Term)
| July 26
|
| July 24, July 26
|
MP122
| E0840
| pSB1A2
|
| gfp tri-part; strong rbs
|
|
| July 26
|
MP123
|
| J61002
|
|
|
|
| August 5
|
MP124
| L102
| pSB1A2
|
| Strong rbs - YFP D129 (BV) - D118 (BI)
|
|
| August 1
|
MP125
| L103
| pSB1A2
|
| Strong rbs - mRFP D129 (BV) - D122 (BI)
|
|
| August 1
|
MP126
| L104
| pSB1A2
|
| Strong rbs - lasR activator D129 (BV) - D114 (BI)
|
|
| August 1
|
MP127
| L105
| J61002
|
| Strong promoter - ECFP D123 (BV) - D130 (BI)
|
|
| August 1
|
MP128
| L106
| J61002
|
| Strong promoter - gfp Tripart D123 (BV) - D131 (BI)
|
|
| August 1
|
MP129
| L107
| J61002
|
| Strong promoter - ECFP D103 (BV) - D130 (BI)
|
|
| August 1
|
MP130
| L108
| J61002
|
| Strong promoter - gfp Tripart D103 (BV) - D131 (BI)
|
|
| August 1
|
MP131
| L110
| J61002
|
| Medium promoter - gfp Tripart D124 (BV) - D131 (BI)
|
|
| August 1
|
MP132
| L111
| J61002
|
| Weak promoter - ECFP D104 (BV) - D130 (BI)
|
|
| August 1
|
MP133
| L112
| J61002
|
| Weak promoter - GFP tripart D104 (BV) - D131 (BI)
|
|
| August 1
|
MP134
| L115
| pSB1A2
|
| pLas - ECFP D105 (BV) - D130 (BI)
|
|
| August 1
|
MP135
| L116
| pSB1A2
|
| pLas - gfp Tripart D105 (BV) - D131 (BI)
|
|
| August 1
|
MP136
| L117
| pSB1AK3
|
| yfp - Double Terminator D125 (FV) - D117 (FI)
|
|
| August 1
|
MP137
| L118
| pSB1AK3
|
| rfp - Double Terminator D125 (FV) - D121 (FI)
|
|
| August 1
|
MP138
| L119
| pSB1AK3
|
| lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI)
|
|
| August 1
|
MP139
| L121
| pSB1A2
|
| pTet - gfp tripart D106 (BV) - D131 (BI)
|
|
| August 1
|
MP140
| L124
| pSB1A2
|
| Strongest RBS - rfp D102 (BV) - D122 (BI)
|
|
| August 1
|
MP141
| L125
| pSB1A2
|
| Strongest RBS - yfp D102 (BV) - D118 (BI)
|
|
| August 1
|
MP142
|
| pSB3K3
|
|
|
|
| August 8
|
MP143.1
| E0240
| pSB1A2
|
| gfp generator
|
| 138
| August 8
|
MP143.2
|
| 150
|
MidiP144
| L128
| J61002
|
| pFlgA-RFP D132 (FI) - D136 (FV)
|
| 538
| August 12
|
MP144.2
| 166
|
MP144.3
| 163
|
MP144.4
| 154
|
MP145.1
| L129
| J61002
|
| pFlgB-RFP D133 (FI) - D136 (FV)
| MP145.6 ; MP145.7
| 136
| August 12
|
MP145.2
| 151
|
MP145.6
| 163
|
MP145.7
| 188
|
MP146.1
| L130
| J61002
|
| pFlhB-RFP D134 (FI) - D136 (FV)
| MP146.1 ; MP146.2
| 298
| August 12
|
MP146.2
| 142
|
MP146.7
| 170
|
MP146.8
| 143
|
MP147
| L100
| pSB1A2
|
| rbs TetR - ECFP D110 (BV) - D130 (BI)
|
|
| August 13
|
MP148.1
| L101
| pSB1A2
|
| rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!!
| MP148.2 ; MP148.3
| 109
| August 13
|
MP148.2
| 123
|
MP148.3
| 123
|
MP149
| L114
| pSB2K3
|
| AracpBAD - gfp tripart D126 (BV) - D131 (BI)
|
|
| August 13
|
MP150
| L120
| pSB1A2
|
| tetR repressible promoter - ECFP D106 (BV) - D130 (BI)
|
|
| August 13
|
MP151
| L122
| pSB1A2
|
| RBS-lasI - ECFP D107 (BV) - D130 (BI)
|
|
| August 13
|
MP152
| L123
| pSB1A2
|
| RBS lasI - gfp tripart D107 (BV) - D131 (BI)
|
|
| August 13
|
MP153.1
| L126
| pSB1A2
|
| Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)
| MP153.1 ; MP153.3
| 100
| August 13
|
MP153.2
| 131
|
MP153.3
| 113
|
MP154
| L132
| pSB1A2
|
| flhDC D142(FV) - D139(FI)
|
|
| August 13
|
MP155
| L133
| pSB1A2
|
| OmpR* D142(FV) - D140(FI)
| WT sequence (August 19)
|
| August 13 ; August 19
|
MP156
| L134
| pSB1A2
|
| EnvZ* D142(FV) - D141(FI)
| lacks about 300 bp in EnvZ* (August 19)
|
| August 13 August 19
|
MP157
| L138
| pSB3K3
|
| gfp generator (E0240) D137(FV) - D138(FI)
|
|
| August 13
|
MP158
| L139
| pSB3K3
|
| E0240 RBS+ D140 (PstI) - D144 (ES)
| seems to have the plasmid without the insert and one with the insert
|
| August 15
|
MP159
| L141
| pSB1A2
|
| flhD D142 (ES) - D145 (ES)
| has not been sequenced
|
| August 15
|
MP160
| L142
| pSB1A2
|
| flhDC+promotor D143 (ES) - D145 (ES)
| does not have the insert
|
| August 15
|
MP161
| L150
| pSB1A2
|
| pLas + rbs-tetR-gfp tripart D105 (SP) - D146 (XP)
| incorrect clone -> to do again
|
| August 19
|
MP162.1
| L151.1
| pSB1AK3
|
| rbs-lasR + double terminator D147 (ES) - D125 (EX)
|
| 151
| August 19
|
MP162.2
|
| 172
|
MP162.3
|
| 149
|
MP163.1
| B0032
| pSB1A2
|
| RBS
|
| 80
| August 20
|
MP163.2
|
| 79
|
MP164
| E0422
| pSB1A2
|
| RBS+ ECFP+ LVA+ term
|
|
| August 20
|
MP165
| E0430
| pSB1A2
|
| RBS+ YFP+ LVA- term
|
|
| August 20
|
MP166
| E0432
| pSB1A2
|
| RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry
|
|
| August 20
|
MP167
| E0420
| pSB1A2
|
| RBS+ ECFP LVA- term
|
|
| August 20
|
MP168
| I732078
| pSB1A3
|
| RBS+ mRFP LVA+ term
|
|
| August 20
|
MP171
| L167
| pSB1A2
| gfp generator(E0240) - pTet
| gfp generator(E0240) - pTet
|
|
| August 25
|
MP172
| L166
| pSB1A2
| RBS(B0032) - tetR
| RBS(B0032) - tetR
|
|
| August 25
|
MP175
| pFlhB
| pCR4Blunt-TOPO
| pFlhB
| pFlhB-RBS (WT)
|
|
| September 04
|
MP176
| pFliA
| pCR4Blunt-TOPO
| pFliA
| pFliA-RBS (WT)
|
|
| September 04
|
MP177
| pFlhDC
| pCR4Blunt-TOPO
| pFlhDC
| pFlhDC-RBS (WT)
|
|
| September 04
|
MP178
| pFliL
| pCR4Blunt-TOPO
| pFliL
| pFliL-RBS (WT)
|
|
| September 04
|
MP179
| RBS WT-FlhDC
| pCR4Blunt-TOPO
| RBS(FlhDC) - FlhDC
| RBS(FlhDC) - FlhDC not mutated for the internal Pst1 site
|
|
| September 04
|
MP180
| FliA2
| pCR4Blunt-TOPO
| FliA
| FliA mutated at EcoR1 and Pst1 internal site
|
|
| September 04
|
MP181
| FliA3
| pCR4Blunt-TOPO
| FliA3
| FliA mutated at EcoR1 and Pst1 internal site
|
|
| September 04
|
MP182
| EnvZ*
| pCR4Blunt-TOPO
| EnvZ*
| EnvZ* the good one not mutated for the internal EcoR1 site
|
|
| September 04
|
MP183
| OmpR WT
| pCR4Blunt-TOPO
| OmpR WT
| OmpR WT not the mutated
|
|
| September 04
|
MP184
| pFlgB
| pCR4Blunt-TOPO
| pFlgB
| pFlgB-RBS (WT)
|
|
| September 04
|
MP185
| OmpR WT
|
| OmpR WT
| OmpR WT not the mutated
|
|
| September 04
|
MP200
| J23101 + RBS (B0032) + tet R
| J61002
|
| J23101 + RBS (B0032) + tet R
|
|
| September 24
|
MP201
| pFlgA-EnvZ*
| J61002
| pFlgA-EnvZ*
| pFlgA(RBS WT)-EnvZ*
|
|
| September 24
|
MP202
| L174; J23101-E0240
| J61002
| Strong constitutive promoter-GFP generator
J23101-E0240
|
|
|
| September 26
|
MP207
| pFliA
| J61002
| pFliA
| pFliA-RBS (WT)
|
|
| October 04
|
MP208
| pFlgA-EnvZ*
| pSB3K3
| pFlgA-EnvZ*
| pFlgA(RBS WT)-EnvZ*
|
|
| October 04
|
MP209
| pFlgA-EnvZ*-mRFP
| J61002
| pFlgA-EnvZ*-mRFP
| pFlgA(RBS WT)-EnvZ*-mRFP
|
|
| October 04
|