Plasmids: MiniPrep Products 2008
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| Digestion
| comment
| Date
|
B0034
| pSB1A2
| S
| MP100
| Strongest RBS (Efficiency = 1)
| x
|
| July 26
|
J23101
| J61002
| S
| MP101
| Strongest Constitutive promoter
| x
|
| July 22, July 23, July 26
|
MP102
| J23109
| J61002
|
| Weak Constitutive promoter
| July 26
|
| July 22, July 26
|
MP103
| R0079
| pSB1A2
|
| promoter pLas (lasr & PAI regulated)
| July 26
|
| July 23, July 26
|
MP104.1
| R0040
| pSB1A2
|
| tetR repressible promoter
| July 26
| 145
| July 23, July 26
|
MP104.3
| 147
|
MP104.4
| 51
|
MP105.1
| S03154
| pSB1A2
|
| B0034(rbs) ->LasI
|
| 34
| July 26
|
MP105.2
|
| 31
|
MP106.1
| S03879
| pSB1A2
|
| B0034(rbs) -> TetR
|
| 80
| August 24
|
MP106.3
|
| 40
|
MP107
| C0079
| pSB2K3
|
| lasR activator with LVA Tag
|
|
| July 23
|
MP108
| C0179
| pSB1A2
|
| lasR activator
| July 26
|
| July 22, July 23, July 26
|
MP109
| E0030
| pSB1AK3
|
| YFP
|
|
| July 26
|
MP110
| E0040
| pSB1A2
|
| GFP
| July 26
|
| July 23, July 26
|
MP111
| E1010
| pSB2K3
|
| mRFP
| July 26
|
| July 23, July 26
|
MP112
| OmpR M57I
|
|
|
|
|
| July 23
|
MP113
| EnvZ I281S
|
|
|
|
|
| July 23
|
MP114.1
| C0040
| pSB1A2
|
| tetR repressor
|
| 173
| July 23
|
MP114.2
|
| 263
|
MP115
| pSB1A3
|
|
|
|
|
| July 23
|
MP116
| J23100
| J61002
|
| Strong constitutive promoter in J61002
|
|
| July 26
|
MP117
| J23107
| J61002
|
| Medium constitutive promoter in J61002
| July 26
|
| July 24, July 26
|
MP118.1
| B0015
| pSB1AK3
|
| Double terminator
| July 26
| 220
| July 24, July 26
|
MP118.3
| 187
|
MP119.1
| I0500
| pSB2K3
|
| AraC pBAD
| July 26
| 42
| July 24, July 26
|
MP119.2
| 26
|
MP119.3
| 39
|
MP120
| B0030
| pSB1A2
|
| Strong RBS (Efficiency = 0,6)
|
|
| July 26
|
MP121
| E0422
| pSB1A2
|
| ECFP (RBS+LVA+Double Term)
| July 26
|
| July 24, July 26
|
MP122
| E0840
| pSB1A2
|
| gfp tri-part; strong rbs
|
|
| July 26
|
MP123
|
| J61002
|
|
|
|
| August 5
|
L100
| pSB1A2
| S146
| MP147
| rbs TetR - ECFP
| D110 (BV) - D130 (BI)
|
| August 13
|
L101
| pSB1A2
| S147
| MP148
| rbs TetR - GFP tripart
| D110 (BV) - D131 (BI)
| DO NOT WORK !!!!
| August 13
|
L102
| pSB1A2
| S123
| MP124
| Strong rbs - YFP
| D129 (BV) - D118 (BI)
|
| August 1
|
L103
| pSB1A2
| S124
| MP125
| Strong rbs - mRFP
| D129 (BV) - D122 (BI)
|
| August 1
|
L104
| pSB1A2
| S125
| MP126
| Strong rbs - lasR activator
| D129 (BV) - D114 (BI)
|
| August 1
|
L105
| J61002
| S126
| MP127
| Strong promoter - ECFP
| D123 (BV) - D130 (BI)
|
| August 1
|
L106
| J61002
| S127
| MP128
| Strong promoter - gfp Tripart
| D123 (BV) - D131 (BI)
|
| August 1
|
L107
| J61002
| S128
| MP129
| Strong promoter - ECFP
| D103 (BV) - D130 (BI)
|
| August 1
|
L108
| J61002
| S129
| MP130
| Strong promoter - gfp Tripart
| D103 (BV) - D131 (BI)
|
| August 1
|
L109
|
|
|
|
|
|
| August
|
L110
| J61002
| S130
| MP131
| Medium promoter - gfp Tripart
| D124 (BV) - D131 (BI)
|
| August 1
|
L111
| J61002
| S131
| MP132
| Weak promoter - ECFP
| D104 (BV) - D130 (BI)
|
| August 1
|
L112
| J61002
| S132
| MP133
| Weak promoter - GFP tripart
| D104 (BV) - D131 (BI)
|
| August 1
|
L113
|
|
|
|
|
|
| August
|
L114
| pSB2K3
| S148
| MP149
| AracpBAD - gfp tripart
| D126 (BV) - D131 (BI)
|
| August 13
|
L115
| pSB1A2
| S133
| MP134
| pLas - ECFP
| D105 (BV) - D130 (BI)
|
| August 1
|
L116
| pSB1A2
| S134
| MP135
| pLas - gfp Tripart
| D105 (BV) - D131 (BI)
|
| August 1
|
L117
| pSB1AK3
| S135
| MP136
| yfp - Double Terminator
| D125 (FV) - D117 (FI)
|
| August 1
|
L118
| pSB1AK3
| S136
| MP137
| rfp - Double Terminator
| D125 (FV) - D121 (FI)
|
| August 1
|
L119
| pSB1AK3
| S137
| MP138
| lasR activator + LVA - Double Terminator
| D125 (FV) - D113 (FI)
|
| August 1
|
L120
| pSB1A2
| S149
| MP150
| tetR repressible promoter - ECFP
| D106 (BV) - D130 (BI)
|
| August 13
|
L121
| pSB1A2
| S138
| MP139
| pTet - gfp tripart
| D106 (BV) - D131 (BI)
|
| August 1
|
L122
| pSB1A2
| S150
| MP151
| RBS-lasI - ECFP
| D107 (BV) - D130 (BI)
|
| August 13
|
L123
| pSB1A2
| S151
| MP152
| RBS lasI - gfp tripart
| D107 (BV) - D131 (BI)
|
| August 13
|
L124
| pSB1A2
| S139
| MP140
| Strongest RBS - rfp
| D102 (BV) - D122 (BI)
|
| August 1
|
L125
| pSB1A2
| S140
| MP141
| Strongest RBS - yfp
| D102 (BV) - D118 (BI)
|
| August 1
|
L126
| pSB1A2
| S152
| MP153
| Strongest RBS (1)- LasR activator (+LVA)
| D102 (BV) - D114 (BI)
|
| August 13
|
L128
| J61002
| S143
| MP144
| pFlgA-RFP
| D132 (FI) - D136 (FV)
|
| August 12
|
L129
| J61002
| S144
| MP145
| pFlgB-RFP
| D133 (FI) - D136 (FV)
|
| August 12
|
L130
| J61002
| S145
| MP146
| pFlhB-RFP
| D134 (FI) - D136 (FV)
| wrong sequencing
| August 12
|
L131
| J61002
|
|
| pFlhDC-RFP
| D136 (FV)- D135 (FI)
| wrong sequencing
| August 8
|
L132
| pSB1A2
| S153
| MP154
| flhDC
| D142(FV) - D139(FI)
|
| August 13
|
L133
| pSB1A2
| S154
| MP155
| OmpR*
| D142(FV) - D140(FI)
| WT sequence (August 19)
| August 13 ; August 19
|
L134
| pSB1A2
| S155
| MP156
| EnvZ*
| D142(FV) - D141(FI)
| lacks about 300 bp in EnvZ* (August 19)
| August 13 August 19
|
L135
| pSB3K3
|
|
| pFlgA
| D137 (BV) - D133 (BI)
| don't work
| August 9
|
L136
| pSB3K3
|
|
| pFlgB
| D137 (BV) - D1343 (BI)
| don't work
| August 9
|
L137
| pSB3K3
|
|
| pFlhB
| D137 (BV) - D135 (BI)
| don't work
| August 9
|
L138
| pSB3K3
| S156
| MP157
| gfp generator (E0240)
| D137(FV) - D138(FI)
|
| August 13
|
L139
| pSB3K3
| S159
| MP158
| E0240 RBS+
| D140 (PstI) - D144 (ES)
| seems to have the plasmid without the insert and one with the insert
| August 15
|
L140
| pSB1A2
|
|
| flhD
| D145 (V) - D141 (I)
|
| August 12
|
L141
| pSB1A2
| S160
| MP159
| flhC
| D142 (ES) - D145 (ES)
| has not been sequenced
| August 15
|
L142
| pSB1A2
| S161
| MP160
| flhDC+promotor
| D143 (ES) - D145 (ES)
| does not have the insert
| August 15
|
L143
| pSB3K3
| x
| x
| pFliL in pSB3K3
| D149 - D137
| didn't work
| August 14
|
L144
| pSB3K3
| x
| x
| pFliL in pSB3K3
| D150 - D152
| didn't work
| August 14
|
L145
| pSB1A2
| x
| x
| g FlhDC in pSB1A2
| D1543 - D145
| didn't work
| August 16
|
L146
| pSB1A2
| x
| x
| g FlhDC in pSB1A2
| D154 - D145
| didn't work
| August 16
|
L147
| J61002
| x
| x
| pFlhDC in J61002
| D155 - D136
| didn't work
| August 16
|
L148
| pSB1A2
| x
| x
| rbs (B0034)- OmpR*
| D158 - D102
| didn't work
| August 16
|
L149
| pSB1A2
| x
| x
| rbs (B0034)- EnvZ
| D159 - D102
| didn't work
| August 16
|
L150
| pSB1A2
| x
| MP161
| pLas + rbs-tetR-gfp tripart
| D105 (BV) - D146 (BI)
| incorrect clone -> to do again
| August 19
|
L151
| pSB1AK3
| S162
| MP162
| rbs-lasR + double terminator
| D147 (FI) - D125 (FV)
|
| August 19
|
L153
| J61002
| S169
| MP169
| pJ23100-rbs-lasER-dble ter
| D123 (BV) - D165 (BI)
|
| August 20
|
L154
| J61002
| S170
| MP170
| pJ23101-rbs-lasER-dble ter
| D103 (BV) - D165 (BI)
|
| August 20
|
L155
|
|
|
| pJ23101-gfp generator
| D164(FV) - D163 (FI)
|
| August 22
|
L156
|
|
|
| pTet-gfp generator
| D161 (FV) - D163 (FI)
|
| August 22
|
L157
|
|
|
| tetR-rbs (B0015)
| D125 (BV) - D162 (BI)
|
| August 23
|
L158
|
| x
| x
| rbs-tetR-gfp generator
| D110(BV) - D131 (BI)
| didn't work
| August 23
|
L159
|
| x
| x
| rbs-lasI-dbl ter
| D125 (FV) - D109 (FI)
| didn't work
| August
|
L160
| J61002
| S174
| MP173
| pFlgA - YFP Tripart (LVA-)
| D166 (FV) - D132 (FI)
| Fluorescence OK
| August 22
|
L161
| J61002
| S175
| MP174
| pFlgA - YFP Tripart (LVA+)
| D167 (FV) - D132 (FI)
|
| August 22
|
L162
| pSB1A2
| x
| x
| rbs-TetR-gfp generator
| D110 (BV) - D163 (BI)
|
| August 22
|
L163
| pSB1A2
| x
| x
| rbs-lasI-gfp generator
| D107 (BV) - D163 (BI)
|
| August 22
|
L166
| pSB1A2
| S173
| MP172
| RBS(B0032) - tetR
|
|
| August 25
|
L167
| pSB1A2
| S172
| MP171
| gfp generator(E0240) - pTet
|
|
| August 25
|
L174
| J61002
| S
| MP202
|
Strong constitutive promoter-GFP generator
| J23101-E0240
|
| September 26
|
L176
|
|
|
|
| D125 (v) + D162 (i)
|
| September
|
L179
| J61002
| S200
| MP200
| J23101 + RBS (B0032) + tet R
|
|
| September 24
|
L180
|
|
|
|
| D166 (v) + D109.2 (i)
|
| September
|
L181
|
|
|
|
| D199 (v) + D109.2 (i)
|
| September
|
L182
|
|
|
|
| D187 (v) + D109.2 (i)
|
| September
|
L186
|
|
|
|
| D179 (v) + D205 (i)
|
| September
|
L190 ("L4")
|
|
|
|
| D199 (v) + D (i)
|
| September
|
L195 ("L9")
|
|
|
|
| D187 (v) + D194 (i)
|
| September
|
L (J23100-B0034 -C0079-B0015)
| J61002
| S169
| MP169
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
L (J23101-B0034 -C0079-B0015)
| J61002
| S170
| MP170
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
L
| pCR4Blunt-TOPO
| S178
| MP175
| pFlhB-RBS (WT)
|
|
| September 04
|
L
| pCR4Blunt-TOPO
| S179
| MP176
| pFliA-RBS (WT)
|
| pFliA with Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S180
| MP177
| pFlhDC-RBS (WT)
|
|
| September 04
|
L
| pCR4Blunt-TOPO
| S81
| MP178
| pFliL-RBS (WT)
|
|
| September 04
|
L
| pCR4Blunt-TOPO
| S182
| MP179
| RBS(FlhDC) - FlhDC
|
| FlhDC with conserved Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S179
| MP180
| FliA
|
| mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S184
| MP181
| FliA3
|
| FliA mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S185
| MP182
| EnvZ*
|
| the good one but not mutated for EcoR1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S186
| MP183
| OmpR WT
|
| OmpR WT not the mutated
| September 04
|
L
| pCR4Blunt-TOPO
| S
| MP184
| pFlgB-RBS (WT)
|
|
| September 04
|
L
| pCR4Blunt-TOPO
| S188
| MP185
| OmpR WT
|
| OmpR WT not the mutated
| September 04
|
L
| J61002
| S201
| MP201
| pFlgA(RBS WT)-EnvZ*
|
|
| September 24
|
L
| J61002
| S207
| MP207
| pFliA
|
|
| October 04
|
L
| pSB3K3
| S208
| MP208
| pFlgA(RBS WT)-EnvZ*
|
| without pFlgA indeed
| October 04
|
L
| J61002
| S209
| MP209
| pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+
|
| without pFlgA indeed
| October 04
|
L
| pSB3K3
| S210
| MP210
| pFliL(RBS WT)
|
|
| October 10
|
L
| pSB3K3
| S211
| MP211
| pFlgA(RBS WT)
|
|
| October 10
|
L
| pSB3K3
| S212
| MP212
| pFlhB(RBS WT)
|
|
| October 10
|
L
| J61002
| S213
| MP213
| pFlhB(RBS WT)-mRFP
pFlhB(RBS WT)-mRFP
|
|
| October 10
|
L
| J61002
| S214
| MP214
| pFliL(RBS WT)-mRFP
|
|
| October 10
|
Plasmids: MiniPrep Products 2008
|
Number
| Name
| Plamsid
| Biobricks
| Description
| Products in use/comments
| Conc. ug/mL
| Date
|
MP100
| B0034
| pSB1A2
|
| Strongest RBS (Efficiency = 1)
|
|
| July 26
|
MP101.1
| J23101
| J61002
|
| Strongest Constitutive promoter
| July 26
| 333
| July 22, July 23, July 26
|
MP101.2
| finished
|
MP101.4
| 200
|
MP102
| J23109
| J61002
|
| Weak Constitutive promoter
| July 26
|
| July 22, July 26
|
MP103
| R0079
| pSB1A2
|
| promoter pLas (lasr & PAI regulated)
| July 26
|
| July 23, July 26
|
MP104.1
| R0040
| pSB1A2
|
| tetR repressible promoter
| July 26
| 145
| July 23, July 26
|
MP104.3
| 147
|
MP104.4
| 51
|
MP105.1
| S03154
| pSB1A2
|
| B0034(rbs) ->LasI
|
| 34
| July 26
|
MP105.2
|
| 31
|
MP106.1
| S03879
| pSB1A2
|
| B0034(rbs) -> TetR
|
| 80
| August 24
|
MP106.3
|
| 40
|
MP107
| C0079
| pSB2K3
|
| lasR activator with LVA Tag
|
|
| July 23
|
MP108
| C0179
| pSB1A2
|
| lasR activator
| July 26
|
| July 22, July 23, July 26
|
MP109
| E0030
| pSB1AK3
|
| YFP
|
|
| July 26
|
MP110
| E0040
| pSB1A2
|
| GFP
| July 26
|
| July 23, July 26
|
MP111
| E1010
| pSB2K3
|
| mRFP
| July 26
|
| July 23, July 26
|
MP112
| OmpR M57I
|
|
|
|
|
| July 23
|
MP113
| EnvZ I281S
|
|
|
|
|
| July 23
|
MP114.1
| C0040
| pSB1A2
|
| tetR repressor
|
| 173
| July 23
|
MP114.2
|
| 263
|
MP115
| pSB1A3
|
|
|
|
|
| July 23
|
MP116
| J23100
| J61002
|
| Strong constitutive promoter in J61002
|
|
| July 26
|
MP117
| J23107
| J61002
|
| Medium constitutive promoter in J61002
| July 26
|
| July 24, July 26
|
MP118.1
| B0015
| pSB1AK3
|
| Double terminator
| July 26
| 220
| July 24, July 26
|
MP118.3
| 187
|
MP119.1
| I0500
| pSB2K3
|
| AraC pBAD
| July 26
| 42
| July 24, July 26
|
MP119.2
| 26
|
MP119.3
| 39
|
MP120
| B0030
| pSB1A2
|
| Strong RBS (Efficiency = 0,6)
|
|
| July 26
|
MP121
| E0422
| pSB1A2
|
| ECFP (RBS+LVA+Double Term)
| July 26
|
| July 24, July 26
|
MP122
| E0840
| pSB1A2
|
| gfp tri-part; strong rbs
|
|
| July 26
|
MP123
|
| J61002
|
|
|
|
| August 5
|
MP124
| L102
| pSB1A2
|
| Strong rbs - YFP D129 (BV) - D118 (BI)
|
|
| August 1
|
MP125
| L103
| pSB1A2
|
| Strong rbs - mRFP D129 (BV) - D122 (BI)
|
|
| August 1
|
MP126
| L104
| pSB1A2
|
| Strong rbs - lasR activator D129 (BV) - D114 (BI)
|
|
| August 1
|
MP127
| L105
| J61002
|
| Strong promoter - ECFP D123 (BV) - D130 (BI)
|
|
| August 1
|
MP128
| L106
| J61002
|
| Strong promoter - gfp Tripart D123 (BV) - D131 (BI)
|
|
| August 1
|
MP129
| L107
| J61002
|
| Strong promoter - ECFP D103 (BV) - D130 (BI)
|
|
| August 1
|
MP130
| L108
| J61002
|
| Strong promoter - gfp Tripart D103 (BV) - D131 (BI)
|
|
| August 1
|
MP131
| L110
| J61002
|
| Medium promoter - gfp Tripart D124 (BV) - D131 (BI)
|
|
| August 1
|
MP132
| L111
| J61002
|
| Weak promoter - ECFP D104 (BV) - D130 (BI)
|
|
| August 1
|
MP133
| L112
| J61002
|
| Weak promoter - GFP tripart D104 (BV) - D131 (BI)
|
|
| August 1
|
MP134
| L115
| pSB1A2
|
| pLas - ECFP D105 (BV) - D130 (BI)
|
|
| August 1
|
MP135
| L116
| pSB1A2
|
| pLas - gfp Tripart D105 (BV) - D131 (BI)
|
|
| August 1
|
MP136
| L117
| pSB1AK3
|
| yfp - Double Terminator D125 (FV) - D117 (FI)
|
|
| August 1
|
MP137
| L118
| pSB1AK3
|
| rfp - Double Terminator D125 (FV) - D121 (FI)
|
|
| August 1
|
MP138
| L119
| pSB1AK3
|
| lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI)
|
|
| August 1
|
MP139
| L121
| pSB1A2
|
| pTet - gfp tripart D106 (BV) - D131 (BI)
|
|
| August 1
|
MP140
| L124
| pSB1A2
|
| Strongest RBS - rfp D102 (BV) - D122 (BI)
|
|
| August 1
|
MP141
| L125
| pSB1A2
|
| Strongest RBS - yfp D102 (BV) - D118 (BI)
|
|
| August 1
|
MP142
|
| pSB3K3
|
| pSB3K3 (low to medium copy plasmid with p15A ORI / kanamycine resistance)
|
|
| August 8
|
MP143.1
| E0240
| pSB1A2
|
| gfp generator
|
| 138
| August 8
|
MP143.2
|
| 150
|
MidiP144
| L128
| J61002
|
| pFlgA-RFP D132 (FI) - D136 (FV)
|
| 538
| August 12
|
MP144.2
| 166
|
MP144.3
| 163
|
MP144.4
| 154
|
MP145.1
| L129
| J61002
|
| pFlgB-RFP D133 (FI) - D136 (FV)
| Wrong sequence for pFlgB
| 136
| August 12
|
MP145.2
| 151
|
MP145.6
| 163
|
MP145.7
| 188
|
MP146.1
| L130
| J61002
|
| pFlhB-RFP D134 (FI) - D136 (FV)
| Wrong sequence for pFlhB
| 298
| August 12
|
MP146.2
| 142
|
MP146.7
| 170
|
MP146.8
| 143
|
MP147
| L100
| pSB1A2
|
| rbs TetR - ECFP D110 (BV) - D130 (BI)
|
|
| August 13
|
MP148.1
| L101
| pSB1A2
|
| rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!!
| MP148.2 ; MP148.3
| 109
| August 13
|
MP148.2
| 123
|
MP148.3
| 123
|
MP149
| L114
| pSB2K3
|
| AracpBAD - gfp tripart D126 (BV) - D131 (BI)
|
|
| August 13
|
MP150
| L120
| pSB1A2
|
| tetR repressible promoter - ECFP D106 (BV) - D130 (BI)
|
|
| August 13
|
MP151
| L122
| pSB1A2
|
| RBS-lasI - ECFP D107 (BV) - D130 (BI)
|
|
| August 13
|
MP152
| L123
| pSB1A2
|
| RBS lasI - gfp tripart D107 (BV) - D131 (BI)
|
|
| August 13
|
MP153.1
| L126
| pSB1A2
|
| Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)
| MP153.1 ; MP153.3
| 100
| August 13
|
MP153.2
| 131
|
MP153.3
| 113
|
MP154
| L132
| pSB1A2
|
| flhDC D142(FV) - D139(FI)
|
|
| August 13
|
MP155
| L133
| pSB1A2
|
| OmpR* D142(FV) - D140(FI)
| WT sequence (August 19)
|
| August 13 ; August 19
|
MP156
| L134
| pSB1A2
|
| EnvZ* D142(FV) - D141(FI)
| lacks about 300 bp in EnvZ* (August 19)
|
| August 13 August 19
|
MP157
| L138
| pSB3K3
|
| gfp generator (E0240) D137(FV) - D138(FI)
|
|
| August 13
|
MP158
| L139
| pSB3K3
|
| E0240 RBS+ D140 (PstI) - D144 (ES)
| seems to have the plasmid without the insert and one with the insert
|
| August 15
|
MP159
| L141
| pSB1A2
|
| flhD D142 (ES) - D145 (ES)
| has not been sequenced
|
| August 15
|
MP160
| L142
| pSB1A2
|
| flhDC+promotor D143 (ES) - D145 (ES)
| does not have the insert
|
| August 15
|
MP161
| L150
| pSB1A2
|
| pLas + rbs-tetR-gfp tripart D105 (SP) - D146 (XP)
| incorrect clone -> to do again
|
| August 19
|
MP162.1
| L151.1
| pSB1AK3
|
| rbs-lasR + double terminator D147 (ES) - D125 (EX)
|
| 151
| August 19
|
MP162.2
|
| 172
|
MP162.3
|
| 149
|
MP163.1
| B0032
| pSB1A2
|
| RBS
|
| 80
| August 20
|
MP163.2
|
| 79
|
MP164
| E0422
| pSB1A2
|
| RBS+ ECFP+ LVA+ term
|
|
| August 20
|
MP165
| E0430
| pSB1A2
|
| RBS+ YFP+ LVA- term
|
|
| August 20
|
MP166
| E0432
| pSB1A2
|
| RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry
|
|
| August 20
|
MP167
| E0420
| pSB1A2
|
| RBS+ ECFP LVA- term
|
|
| August 20
|
MP168
| I732078
| pSB1A3
|
| RBS+ mRFP LVA+ term
|
|
| August 20
|
MP169
| J23100-B0034 -C0079-B0015
| J61002
|
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
MP170
| J23101-B0034 -C0079-B0015
| J61002
|
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
MP171
| L167
| pSB1A2
| gfp generator(E0240) - pTet
| gfp generator(E0240) - pTet
|
|
| August 25
|
MP172
| L166
| pSB1A2
| RBS(B0032) - tetR
| RBS(B0032) - tetR
|
|
| August 25
|
MP173
| L160
| J61002
| pFlgA-YFP tripart LVA-
| pFlgA-YFP tripart LVA- term
|
|
| August 26
|
MP174
| L161
| J61002
| pFlgA-YFP tripart LVA+
| pFlgA-YFP tripart LVA+ term
|
|
| August 26
|
MP175
| pFlhB
| pCR4Blunt-TOPO
| pFlhB
| pFlhB-RBS (WT)
|
|
| September 04
|
MP176
| pFliA
| pCR4Blunt-TOPO
| pFliA
| pFliA-RBS (WT) with conserved internal Pst1 restriction site
|
|
| September 04
|
MP177
| pFlhDC
| pCR4Blunt-TOPO
| pFlhDC
| pFlhDC-RBS (WT)
|
|
| September 04
|
MP178
| pFliL
| pCR4Blunt-TOPO
| pFliL
| pFliL-RBS (WT)
|
|
| September 04
|
MP179
| RBS WT-FlhDC
| pCR4Blunt-TOPO
| RBS(FlhDC) - FlhDC
| RBS(FlhDC) - FlhDC with conserved internal Pst1 restriction site
|
|
| September 04
|
MP180
| FliA2
| pCR4Blunt-TOPO
| FliA
| FliA mutated at EcoR1 and Pst1 internal site
|
|
| September 04
|
MP181
| FliA3
| pCR4Blunt-TOPO
| FliA3
| FliA mutated at EcoR1 and Pst1 internal site
|
|
| September 04
|
MP182
| EnvZ*
| pCR4Blunt-TOPO
| EnvZ*
| EnvZ* the good one but not mutated for the internal EcoR1 restriction site
|
|
| September 04
|
MP183
| OmpR WT
| pCR4Blunt-TOPO
| OmpR WT
| OmpR WT not the mutated
|
|
| September 04
|
MP184
| pFlgB
| pCR4Blunt-TOPO
| pFlgB
| pFlgB-RBS (WT)
|
|
| September 04
|
MP185
| OmpR WT
|
| OmpR WT
| OmpR WT not the mutated
|
|
| September 04
|
MP200
| J23101 + RBS (B0032) + tet R
| J61002
|
| J23101 + RBS (B0032) + tet R
|
|
| September 24
|
MP201
| pFlgA-EnvZ*
| J61002
| pFlgA-EnvZ*
| pFlgA(RBS WT)-EnvZ*
|
|
| September 24
|
MP202
| L174; J23101-E0240
| J61002
| Strong constitutive promoter-GFP generator
J23101-E0240
|
|
|
| September 26
|
MP207
| pFliA
| J61002
| pFliA
| pFliA
|
|
| October 04
|
MP208
| pFlgA-EnvZ*
| pSB3K3
| pFlgA-EnvZ*
| pFlgA(RBS WT)-EnvZ*
| without pFlgA indeed
|
| October 04
|
MP209
| pFlgA-EnvZ*-I732078
| J61002
| pFlgA-EnvZ*-mRFP tripart
| pFlgA(RBS WT)-EnvZ*-mRFP tripart
| without pFlgA indeed
|
| October 04
|
MP210
| pFliL
| pSB3K3
| pFliL
| pFliL(RBS WT)
|
|
| October 10
|
MP211
| pFlgA
| pSB3K3
| pFlgA
| pFlgA(RBS WT)
|
|
| October 10
|
MP212
| pFlhB
| pSB3K3
| pFlhB
| pFlhB(RBS WT)
|
|
| October 10
|
MP213
| pFlhB
| J61002
| pFlhB(RBS WT)-mRFP
| pFlhB(RBS WT)-mRFP
|
|
| October 10
|
MP214
| pFliL
| J61002
| pFliL(RBS WT)-mRFP
| pFliL(RBS WT)-mRFP
|
|
| October 10
|
MP215
|
| pSB4T5
|
| pSB4T5 (low copy plasmid with pSC101 ORI / tetracycline resistance)
|
|
| October 14
|
MP216
| E0434
| pSB1AK3
|
| YFP tripart +AAV
|
|
| October 15
|