BioBricks
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| comment
| Date
|
B0034
| pSB1A2
| S107
| MP100
| Strongest RBS (Efficiency = 1)
|
| July 26
|
J23101
| J61002
| S109
| MP101
| Strongest Constitutive promoter
|
| July 22, July 23, July 26
|
J23109
| J61002
| S110
| MP102
| Weak Constitutive promoter
|
| July 22, July 26
|
R0079
| pSB1A2
| S108
| MP103
| promoter pLas (lasr & PAI regulated)
|
| July 23, July 26
|
R0040
| pSB1A2
| S111
| MP104
| tetR repressible promoter
|
| July 23, July 26
|
S03154
| pSB1A2
| S112
| MP105
| B0034(rbs) ->LasI
|
| July 26
|
S03879
| pSB1A2
| S113
| MP106
| B0034(rbs) -> TetR
|
| August 24
|
C0079
| pSB2K3
| S114
| MP107
| lasR activator with LVA Tag
|
| July 23
|
C0179
| pSB1A2
| S115
| MP108
| lasR activator
|
| July 22, July 23, July 26
|
E0030
| pSB1AK3
| S116
| MP109
| YFP
|
| July 26
|
E0040
| pSB1A2
| S117
| MP110
| GFP
|
| July 23, July 26
|
E1010
| pSB2K3
| S118
| MP111
| mRFP
|
| July 23, July 26
|
OmpR M57I
|
| S119
| MP112
| OmpR
|
| July 23
|
EnvZ I281S
|
| S120
| MP113
| EnvZ
|
| July 23
|
C0040
| pSB1A2
| S121
| MP114
| tetR repressor
|
| July 23
|
pSB1A3
| pSB1A3
| S122
| MP115
| plasmid
|
| July 23
|
J23100
| J61002
| x
| MP116
| Strong constitutive promoter
|
| July 26
|
J23107
| J61002
| x
| MP117
| Medium constitutive promoter
|
| July 24, July 26
|
B0015
| pSB1AK3
| x
| MP118
| Double terminator
|
| July 24, July 26
|
I0500
| pSB2K3
| S103
| MP119
| align="center"|AraC pBAD
|
| July 24, July 26
|
B0030
| pSB1A2
| x
| MP120
| Strong RBS (Efficiency = 0,6)
|
| July 26
|
E0422
| pSB1A2
| S164
| MP121
| ECFP (RBS+LVA+Double Term)
|
| July 24, July 26
|
E0840
| pSB1A2
| S106
| MP122
| gfp tri-part; strong rbs
|
| July 26
|
J61002
| J61002
| S142
| MP123
| plasmid
|
| August 5
|
pSB3K3
| pSB3K3 (low to medium copy plasmid with p15A ORI / kanamycine resistance)
| x
| MP142
|
|
| August 8
|
E0240
| pSB1A2
| x
| MP143
| gfp generator
|
| August 8
|
B0032
| pSB1A2
| S163
| MP163
| medium rbs (efficiency = 0,3)
|
| August 20
|
E0422
| pSB1A2
| x
| MP164
| RBS+ ECFP+ LVA+ term
|
| August 20
|
E0430
| pSB1A2
| S165
| MP165
|
| RBS+ YFP+ LVA- term
|
| August 20
|
E0432
| pSB1A2
| S166
| MP166
| RBS+ YFP LVA+ term
| BUT be careful , it doesn't work. See registry
| August 20
|
MP167
| E0420
| pSB1A2
| S167
| MP167
| RBS+ ECFP LVA- term
|
| August 20
|
MP168
| I732078
| pSB1A3
| S168
| MP168
| RBS+ mRFP LVA+ term
|
| August 20
|
pFlhB
| pCR4Blunt-TOPO
| x
| MP175
| pFlhB-RBS (WT)
|
| September 04
|
pFliA
| pCR4Blunt-TOPO
| x
| MP176
| pFliA-RBS (WT) with conserved internal Pst1 restriction site
|
| September 04
|
pFlhDC
| pCR4Blunt-TOPO
| x
| MP177
| pFlhDC-RBS (WT)
|
| September 04
|
style="background: #D4E2EF;" pFliL
| pCR4Blunt-TOPO
| x
| MP178
| pFliL-RBS (WT)
|
| September 04
|
RBS WT-FlhDC
| pCR4Blunt-TOPO
| x
| MP179
| RBS(FlhDC) - FlhDC
| with conserved Pst1 site
| September 04
|
FliA2
| pCR4Blunt-TOPO
| S183
| MP180
| FliA2
| mutated at EcoR1 and Pst1 internal site
| September 04
|
FliA3
| pCR4Blunt-TOPO
| x
| MP181
| >br>FliA3
| mutated at EcoR1 and Pst1 site
| September 04
|
EnvZ*
| pCR4Blunt-TOPO
| x
| MP182
| EnvZ*
| the good one but not mutated for EcoR1 site
| September 04
|
OmpR WT
| pCR4Blunt-TOPO
| x
| MP183
| OmpR WT
| not the mutated
| September 04
|
pFlgB
| pCR4Blunt-TOPO
| S187
| MP184
| pFlgB-RBS (WT)
|
| September 04
|
OmpR WT
|
| x
| MP185
| OmpR WT
| not the mutated
| September 04
|