BioBricks
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| comment
| Date
|
MG1655
| Strain
| S141
|
| Strain
|
| Aug 7
|
pSB4T5
| pSB4T5
| S215
| MP215
| plasmid
| pSB4T5 (low copy plasmid with pSC101 ORI / tetracycline resistance)
|
| October 14
|
pSB1A3
| pSB1A3
| S122
| MP115
| plasmid
|
| July 23
|
J61002
| J61002
| S142
| MP123
| plasmid
|
| August 5
|
pSB3K3
| pSB3K3
| S158
| x
| plasmid
|
| August 15
|
pSB3K3
| pSB3K3
| x
| MP142
| plasmid
| low to medium copy plasmid with p15A ORI
| August 8
|
I0500
| pSB2K3
| S103
| MP119
| AraC pBAD
|
| July 24, July 26
|
J23100
| J61002
| x
| MP116
| Strong constitutive promoter
|
| July 26
|
J23107
| J61002
| x
| MP117
| Medium constitutive promoter
|
| July 24, July 26
|
L135
| pSB3K3
|
|
| pFlgA
| D137 (BV) - D133 (BI) don't work
| August 9
|
L136
| pSB3K3
|
|
| pFlgB
| D137 (BV) - D1343 (BI) don't work
| August 9
|
L137
| pSB3K3
|
|
| pFlhB
| D137 (BV) - D135 (BI) don't work
| August 9
|
L143
| pSB3K3
| x
| x
| pFliL
| D149 - D137 didn't work
| August 14
|
L144
| pSB3K3
| x
| x
| pFliL
| D150 - D152 didn't work
| August 14
|
L147
| J61002
| x
| x
| pFlhDC
| D155 - D136 didn't work
| August 16
|
L
| J61002
| S207
| MP207
| pFliA
|
| October 04
|
J23101
| J61002
| S109
| MP101
| Strongest Constitutive promoter
|
| July 22, July 23, July 26
|
J23109
| J61002
| S110
| MP102
| Weak Constitutive promoter
|
| July 22, July 26
|
R0079
| pSB1A2
| S108
| MP103
| promoter pLas (lasr & PAI regulated)
|
| July 23, July 26
|
R0040
| pSB1A2
| S111
| MP104
| tetR repressible promoter
|
| July 23, July 26
|
pFlhB
| pCR4Blunt-TOPO
| x
| MP175
| pFlhB-RBS (WT)
|
| September 04
|
pFliA
| pCR4Blunt-TOPO
| x
| MP176
| pFliA-RBS (WT)
| with conserved Pst1 site
| September 04
|
pFlhDC
| pCR4Blunt-TOPO
| x
| MP177
| pFlhDC-RBS (WT)
|
| September 04
|
pFliL
| pCR4Blunt-TOPO
| x
| MP178
| pFliL-RBS (WT)
|
| September 04
|
pFlgB
| pCR4Blunt-TOPO
| S187
| MP184
| pFlgB-RBS (WT)
|
| September 04
|
L
| pSB3K3
| S210
| MP210
| pFliL(RBS WT)
|
| October 10
|
L
| pSB3K3
| S211
| MP211
| pFlgA(RBS WT)
|
| October 10
|
L
| pSB3K3
| S212
| MP212
| pFlhB(RBS WT)
|
| October 10
|
L
| pCR4Blunt-TOPO
| S179
| MP176
| pFliA-RBS (WT)
| pFliA with Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S180
| MP177
| pFlhDC-RBS (WT)
|
| September 04
|
L
| pCR4Blunt-TOPO
| S181
| MP178
| pFliL-RBS (WT)
|
| September 04
|
B0034
| pSB1A2
| S107
| MP100
| Strongest RBS (Efficiency = 1)
|
| July 26
|
B0032
| pSB1A2
| S163
| MP163
| medium rbs (efficiency = 0,3)
|
| August 20
|
B0030
| pSB1A2
| x
| MP120
| Strong RBS (Efficiency = 0,6)
|
| July 26
|
S03154
| pSB1A2
| S112
| MP105
| B0034(rbs) ->LasI
|
| July 26
|
S03879
| pSB1A2
| S113
| MP106
| B0034(rbs) -> TetR
|
| August 24
|
L
| pCR4Blunt-TOPO
| S182
| MP179
| RBS(FlhDC) - FlhDC
| FlhDC with conserved Pst1 site
| September 04
|
C0079
| pSB2K3
| S114
| MP107
| lasR activator with LVA Tag
|
| July 23
|
C0179
| pSB1A2
| S115
| MP108
| lasR activator
|
| July 22, July 23, July 26
|
OmpR M57I
|
| S119
| MP112
| OmpR
|
| July 23
|
EnvZ I281S
|
| S120
| MP113
| EnvZ
|
| July 23
|
C0040
| pSB1A2
| S121
| MP114
| tetR repressor
|
| July 23
|
FliA2
| pCR4Blunt-TOPO
| S183
| MP180
| FliA2
| mutated at EcoR1 and Pst1 internal site
| September 04
|
FliA3
| pCR4Blunt-TOPO
| x
| MP181
| FliA3
| mutated at EcoR1 and Pst1 site
| September 04
|
EnvZ*
| pCR4Blunt-TOPO
| x
| MP182
| EnvZ*
| the good one but not mutated for EcoR1 site
| September 04
|
OmpR WT
| pCR4Blunt-TOPO
| x
| MP183
| OmpR WT
| not the mutated
| September 04
|
OmpR WT
|
| x
| MP185
| OmpR WT
| not the mutated
| September 04
|
L132
| pSB1A2
| S153
| MP154
| flhDC
| D142(FV) - D139(FI)
| August 13
|
L133
| pSB1A2
| S154
| MP155
| OmpR*
| D142(FV) - D140(FI) WT sequence
| August 19
|
L134
| pSB1A2
| S155
| MP156
| EnvZ*
| D142(FV) - D141(FI) lacks about 300 bp in EnvZ*
| August 19
|
L140
| pSB1A2
|
|
| flhD
| D145 (V) - D141 (I)
| August 12
|
L141
| pSB1A2
| S160
| MP159
| flhC
| D142 (ES) - D145 (ES) has not been sequenced
| August 15
|
L145
| pSB1A2
| x
| x
| FlhDC
| D1543 - D145 didn't work
| August 16
|
L146
| pSB1A2
| x
| x
| FlhDC
| D154 - D145 didn't work
| August 16
|
L
| pCR4Blunt-TOPO
| S179
| MP180
| FliA
| mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S184
| MP181
| FliA3
| FliA mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S185
| MP182
| EnvZ*
| the good one but not mutated for EcoR1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S186
| MP183
| OmpR WT
| OmpR WT not the mutated
| September 04
|
L
| pCR4Blunt-TOPO
| S188
| MP185
| OmpR WT
| OmpR WT not the mutated
| September 04
|
E0030
| pSB1AK3
| S116
| MP109
| YFP
|
| July 26
|
E0040
| pSB1A2
| S117
| MP110
| GFP
|
| July 23, July 26
|
E1010
| pSB2K3
| S118
| MP111
| mRFP
|
| July 23, July 26
|
E0422
| pSB1A2
| S164
| MP121
| ECFP (RBS+LVA+Double Term)
|
| July 24, July 26
|
E0840
| pSB1A2
| S106
| MP122
| gfp tri-part; strong rbs
|
| July 26
|
E0430
| pSB1A2
| S165
| MP165
| RBS+ YFP+ LVA- term
|
| August 20
|
E0432
| pSB1A2
| S166
| MP166
| RBS+ YFP LVA+ term
| BUT be careful , it doesn't work. See registry
| August 20
|
E0420
| pSB1A2
| S167
| MP167
| RBS+ ECFP LVA- term
|
| August 20
|
I732078
| pSB1A3
| S168
| MP168
| RBS+ mRFP LVA+ term
|
| August 20
|
E0240
| pSB1A2
| S157
| x
| GFP generator
|
| August 15
|
E0434
| pSB1AK3
| S216
| x
| YFP tripart +AAV
|
| October 15
|
E0240
| pSB1A2
| x
| MP143
| gfp generator
|
| August 8
|
E0422
| pSB1A2
| x
| MP164
| RBS+ ECFP+ LVA+ term
|
| August 20
|
B0015
| pSB1AK3
| x
| MP118
| Double terminator
|
| July 24, July 26
|
Ligations: Constructions realised
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| Digestion
| comment
| Date
|
L100
| pSB1A2
| S146
| MP147
| rbs TetR - ECFP
| D110 (BV) - D130 (BI)
|
| August 13
|
L101
| pSB1A2
| S147
| MP148
| rbs TetR - GFP tripart
| D110 (BV) - D131 (BI)
| DO NOT WORK !!!!
| August 13
|
L102
| pSB1A2
| S123
| MP124
| Strong rbs - YFP
| D129 (BV) - D118 (BI)
|
| August 1
|
L103
| pSB1A2
| S124
| MP125
| Strong rbs - mRFP
| D129 (BV) - D122 (BI)
|
| August 1
|
L104
| pSB1A2
| S125
| MP126
| Strong rbs - lasR activator
| D129 (BV) - D114 (BI)
|
| August 1
|
L105
| J61002
| S126
| MP127
| Strong promoter - ECFP
| D123 (BV) - D130 (BI)
|
| August 1
|
L106
| J61002
| S127
| MP128
| Strong promoter - gfp Tripart
| D123 (BV) - D131 (BI)
|
| August 1
|
L107
| J61002
| S128
| MP129
| Strong promoter - ECFP
| D103 (BV) - D130 (BI)
|
| August 1
|
L108
| J61002
| S129
| MP130
| Strong promoter - gfp Tripart
| D103 (BV) - D131 (BI)
|
| August 1
|
L109
|
|
|
|
|
|
| August
|
L110
| J61002
| S130
| MP131
| Medium promoter - gfp Tripart
| D124 (BV) - D131 (BI)
|
| August 1
|
L111
| J61002
| S131
| MP132
| Weak promoter - ECFP
| D104 (BV) - D130 (BI)
|
| August 1
|
L112
| J61002
| S132
| MP133
| Weak promoter - GFP tripart
| D104 (BV) - D131 (BI)
|
| August 1
|
L113
|
|
|
|
|
|
| August
|
L114
| pSB2K3
| S148
| MP149
| AracpBAD - gfp tripart
| D126 (BV) - D131 (BI)
|
| August 13
|
L115
| pSB1A2
| S133
| MP134
| pLas - ECFP
| D105 (BV) - D130 (BI)
|
| August 1
|
L116
| pSB1A2
| S134
| MP135
| pLas - gfp Tripart
| D105 (BV) - D131 (BI)
|
| August 1
|
L117
| pSB1AK3
| S135
| MP136
| yfp - Double Terminator
| D125 (FV) - D117 (FI)
|
| August 1
|
L118
| pSB1AK3
| S136
| MP137
| rfp - Double Terminator
| D125 (FV) - D121 (FI)
|
| August 1
|
L119
| pSB1AK3
| S137
| MP138
| lasR activator + LVA - Double Terminator
| D125 (FV) - D113 (FI)
|
| August 1
|
L120
| pSB1A2
| S149
| MP150
| tetR repressible promoter - ECFP
| D106 (BV) - D130 (BI)
|
| August 13
|
L121
| pSB1A2
| S138
| MP139
| pTet - gfp tripart
| D106 (BV) - D131 (BI)
|
| August 1
|
L122
| pSB1A2
| S150
| MP151
| RBS-lasI - ECFP
| D107 (BV) - D130 (BI)
|
| August 13
|
L123
| pSB1A2
| S151
| MP152
| RBS lasI - gfp tripart
| D107 (BV) - D131 (BI)
|
| August 13
|
L124
| pSB1A2
| S139
| MP140
| Strongest RBS - rfp
| D102 (BV) - D122 (BI)
|
| August 1
|
L125
| pSB1A2
| S140
| MP141
| Strongest RBS - yfp
| D102 (BV) - D118 (BI)
|
| August 1
|
L126
| pSB1A2
| S152
| MP153
| Strongest RBS (1)- LasR activator (+LVA)
| D102 (BV) - D114 (BI)
|
| August 13
|
L128
| J61002
| S143
| MP144
| pFlgA-RFP
| D132 (FI) - D136 (FV)
|
| August 12
|
L129
| J61002
| S144
| MP145
| pFlgB-RFP
| D133 (FI) - D136 (FV)
|
| August 12
|
L130
| J61002
| S145
| MP146
| pFlhB-RFP
| D134 (FI) - D136 (FV)
| wrong sequencing
| August 12
|
L131
| J61002
|
|
| pFlhDC-RFP
| D136 (FV)- D135 (FI)
| wrong sequencing
| August 8
|
|
L138
| pSB3K3
| S156
| MP157
| gfp generator (E0240)
| D137(FV) - D138(FI)
|
| August 13
|
L139
| pSB3K3
| S159
| MP158
| E0240 RBS+
| D140 (PstI) - D144 (ES)
| seems to have the plasmid without the insert and one with the insert
| August 15
|
L142
| pSB1A2
| S161
| MP160
| flhDC+promotor
| D143 (ES) - D145 (ES)
| does not have the insert
| August 15
|
L148
| pSB1A2
| x
| x
| rbs (B0034)- OmpR*
| D158 - D102
| didn't work
| August 16
|
L149
| pSB1A2
| x
| x
| rbs (B0034)- EnvZ
| D159 - D102
| didn't work
| August 16
|
L150
| pSB1A2
| x
| MP161
| pLas + rbs-tetR-gfp tripart
| D105 (BV) - D146 (BI)
| incorrect clone -> to do again
| August 19
|
L151
| pSB1AK3
| S162
| MP162
| rbs-lasR + double terminator
| D147 (FI) - D125 (FV)
|
| August 19
|
L153
| J61002
| S169
| MP169
| pJ23100-rbs-lasER-dble ter
| D123 (BV) - D165 (BI)
|
| August 20
|
L154
| J61002
| S170
| MP170
| pJ23101-rbs-lasER-dble ter
| D103 (BV) - D165 (BI)
|
| August 20
|
L155
|
|
|
| pJ23101-gfp generator
| D164(FV) - D163 (FI)
|
| August 22
|
L156
|
|
|
| pTet-gfp generator
| D161 (FV) - D163 (FI)
|
| August 22
|
L157
|
|
|
| tetR-rbs (B0015)
| D125 (BV) - D162 (BI)
|
| August 23
|
L158
|
| x
| x
| rbs-tetR-gfp generator
| D110(BV) - D131 (BI)
| didn't work
| August 23
|
L159
|
| x
| x
| rbs-lasI-dbl ter
| D125 (FV) - D109 (FI)
| didn't work
| August
|
L160
| J61002
| S174
| MP173
| pFlgA - YFP Tripart (LVA-)
| D166 (FV) - D132 (FI)
| Fluorescence OK
| August 22
|
L161
| J61002
| S175
| MP174
| pFlgA - YFP Tripart (LVA+)
| D167 (FV) - D132 (FI)
|
| August 22
|
L162
| pSB1A2
| x
| x
| rbs-TetR-gfp generator
| D110 (BV) - D163 (BI)
|
| August 22
|
L163
| pSB1A2
| x
| x
| rbs-lasI-gfp generator
| D107 (BV) - D163 (BI)
|
| August 22
|
L166
| pSB1A2
| S173
| MP172
| RBS(B0032) - tetR
|
|
| August 25
|
L167
| pSB1A2
| S172
| MP171
| gfp generator(E0240) - pTet
|
|
| August 25
|
L174
| J61002
| S
| MP202
|
Strong constitutive promoter-GFP generator
| J23101-E0240
|
| September 26
|
L176
|
|
|
|
| D125 (v) + D162 (i)
|
| September
|
L179
| J61002
| S200
| MP200
| J23101 + RBS (B0032) + tet R
|
|
| September 24
|
L180
|
|
|
|
| D166 (v) + D109.2 (i)
|
| September
|
L181
|
|
|
|
| D199 (v) + D109.2 (i)
|
| September
|
L182
|
|
|
|
| D187 (v) + D109.2 (i)
|
| September
|
L186
|
|
|
|
| D179 (v) + D205 (i)
|
| September
|
L190 ("L4")
|
|
|
|
| D199 (v) + D (i)
|
| September
|
L195 ("L9")
|
|
|
|
| D187 (v) + D194 (i)
|
| September
|
L (J23100-B0034 -C0079-B0015)
| J61002
| S169
| MP169
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
L (J23101-B0034 -C0079-B0015)
| J61002
| S170
| MP170
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
L
| pCR4Blunt-TOPO
| S178
| MP175
| pFlhB-RBS (WT)
|
|
| September 04
|
L
| pCR4Blunt-TOPO
| S
| MP184
| pFlgB-RBS (WT)
|
|
| September 04
|
L
| J61002
| S201
| MP201
| pFlgA(RBS WT)-EnvZ*
|
|
| September 24
|
L
| pSB3K3
| S208
| MP208
| pFlgA(RBS WT)-EnvZ*
|
| without pFlgA indeed
| October 04
|
L
| J61002
| S209
| MP209
| pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+
|
| without pFlgA indeed
| October 04
|
L
| J61002
| S213
| MP213
| pFlhB(RBS WT)-mRFP
pFlhB(RBS WT)-mRFP
|
|
| October 10
|
L
| J61002
| S214
| MP214
| pFliL(RBS WT)-mRFP
|
|
| October 10
|
RBS WT-FlhDC
| pCR4Blunt-TOPO
| x
| MP179
| RBS(FlhDC) - FlhDC
| with conserved Pst1 site
| September 04
|