BioBricks
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| comment
| Date
|
MG1655
| Strain
| S141
|
| Strain
|
| Aug 7
|
J61002
| J61002
| S142
| MP123
| plasmid
|
| August 5
|
pSB1A3
| pSB1A3
| S122
| MP115
| plasmid
|
| July 23
|
pSB4T5
| pSB4T5
| S215
| MP215
| plasmid
| low copy plasmid with pSC101 ORI
| October 14
|
pSB3K3
| pSB3K3
| S158
| MP142
| plasmid
| low to medium copy plasmid with p15A ORI
| August 15
|
J23100
| J61002
| x
| MP116
| Strong constitutive promoter
|
| July 26
|
J23101
| J61002
| S109
| MP101
| Strongest Constitutive promoter
|
| July 22, July 23, July 26
|
J23107
| J61002
| x
| MP117
| Medium constitutive promoter
|
| July 24, July 26
|
J23109
| J61002
| S110
| MP102
| Weak Constitutive promoter
|
| July 22, July 26
|
I0500
| pSB2K3
| S103
| MP119
| AraC pBAD
|
| July 24, July 26
|
R0040
| pSB1A2
| S111
| MP104
| tetR repressible promoter
|
| July 23, July 26
|
R0079
| pSB1A2
| S108
| MP103
| promoter pLas (lasr & PAI regulated)
|
| July 23, July 26
|
L135
| pSB3K3
|
|
| pFlgA
| D137 (BV) - D133 (BI) don't work
| August 9
|
L136
| pSB3K3
|
|
| pFlgB
| D137 (BV) - D1343 (BI) don't work
| August 9
|
L137
| pSB3K3
|
|
| pFlhB
| D137 (BV) - D135 (BI) don't work
| August 9
|
L147
| J61002
| x
| x
| pFlhDC
| D155 - D136 didn't work
| August 16
|
L
| J61002
| S207
| MP207
| pFliA
|
| October 04
|
L143
| pSB3K3
| x
| x
| pFliL
| D149 - D137 didn't work
| August 14
|
L144
| pSB3K3
| x
| x
| pFliL
| D150 - D152 didn't work
| August 14
|
L
| pSB3K3
| S211
| MP211
| pFlgA(RBS WT)
|
| October 10
|
pFlgB
| pCR4Blunt-TOPO
| S187
| MP184
| pFlgB-RBS (WT)
|
| September 04
|
pFlhB
| pCR4Blunt-TOPO
| x
| MP175
| pFlhB-RBS (WT)
|
| September 04
|
L
| pSB3K3
| S212
| MP212
| pFlhB(RBS WT)
|
| October 10
|
L
| pCR4Blunt-TOPO
| S180
| MP177
| pFlhDC-RBS (WT)
|
| September 04
|
L
| pCR4Blunt-TOPO
| S179
| MP176
| pFliA-RBS (WT)
| pFliA with conserved Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S181
| MP178
| pFliL-RBS (WT)
|
| September 04
|
L
| pSB3K3
| S210
| MP210
| pFliL(RBS WT)
|
| October 10
|
B0030
| pSB1A2
| x
| MP120
| Strong RBS (Efficiency = 0,6)
|
| July 26
|
B0032
| pSB1A2
| S163
| MP163
| medium rbs (efficiency = 0,3)
|
| August 20
|
B0034
| pSB1A2
| S107
| MP100
| Strongest RBS (Efficiency = 1)
|
| July 26
|
S03154
| pSB1A2
| S112
| MP105
| B0034(rbs) ->LasI
|
| July 26
|
S03879
| pSB1A2
| S113
| MP106
| B0034(rbs) -> TetR
|
| August 24
|
L
| pCR4Blunt-TOPO
| S182
| MP179
| RBS(FlhDC) - FlhDC
| FlhDC with conserved Pst1 site
| September 04
|
C0040
| pSB1A2
| S121
| MP114
| tetR repressor
|
| July 23
|
C0079
| pSB2K3
| S114
| MP107
| lasR activator with LVA Tag
|
| July 23
|
C0179
| pSB1A2
| S115
| MP108
| lasR activator
|
| July 22, July 23, July 26
|
EnvZ I281S
| Strain
| S120
| MP113
| EnvZ
|
| July 23
|
OmpR M57I
| Strain
| S119
| MP112
| OmpR
|
| July 23
|
L132
| pSB1A2
| S153
| MP154
| flhDC
| D142(FV) - D139(FI)
| August 13
|
L133
| pSB1A2
| S154
| MP155
| OmpR*
| D142(FV) - D140(FI) WT sequence
| August 19
|
L134
| pSB1A2
| S155
| MP156
| EnvZ*
| D142(FV) - D141(FI) lacks about 300 bp in EnvZ*
| August 19
|
L140
| pSB1A2
|
|
| flhD
| D145 (V) - D141 (I)
| August 12
|
L141
| pSB1A2
| S160
| MP159
| flhC
| D142 (ES) - D145 (ES) has not been sequenced
| August 15
|
L145
| pSB1A2
| x
| x
| FlhDC
| D1543 - D145 didn't work
| August 16
|
L146
| pSB1A2
| x
| x
| FlhDC
| D154 - D145 didn't work
| August 16
|
L
| pCR4Blunt-TOPO
| S183
| MP180
| FliA2
| mutated at EcoR1 and Pst1 internal site
| September 04
|
L
| pCR4Blunt-TOPO
| S184
| MP181
| FliA3
| FliA mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S185
| MP182
| EnvZ*
| the good one but not mutated for EcoR1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S186
| MP183
| OmpR WT
| OmpR WT not the mutated
| September 04
|
L
| pCR4Blunt-TOPO
| S188
| MP185
| OmpR WT
| OmpR WT not the mutated
| September 04
|
E0030
| pSB1AK3
| S116
| MP109
| YFP
|
| July 26
|
E0040
| pSB1A2
| S117
| MP110
| GFP
|
| July 23, July 26
|
E1010
| pSB2K3
| S118
| MP111
| mRFP
|
| July 23, July 26
|
E0240
| pSB1A2
| S157
| MP143
| GFP generator
|
| August 15
|
E0420
| pSB1A2
| S167
| MP167
| RBS+ ECFP LVA- term
|
| August 20
|
E0422
| pSB1A2
| S164
| MP121 MP164
| RBS+ ECFP+ LVA+ term
|
| July 24, July 26 August 20
|
E0430
| pSB1A2
| S165
| MP165
| RBS+ YFP+ LVA- term
|
| August 20
|
E0432
| pSB1A2
| S166
| MP166
| RBS+ YFP LVA+ term
| BUT be careful , it doesn't work. See registry
| August 20
|
E0434
| pSB1AK3
| S216
| MP216
| YFP tripart +AAV
|
| October 15
|
E0840
| pSB1A2
| S106
| MP122
| gfp tri-part; strong rbs
|
| July 26
|
I732078
| pSB1A3
| S168
| MP168
| RBS+ mRFP LVA+ term
|
| August 20
|
B0015
| pSB1AK3
| x
| MP118
| Double terminator
|
| July 24, July 26
|