Latest revision as of 14:49, 13 September 2008

Cloning of EnvZ*

Transformation results of L165 (EnvZ*-pSB1A2) from August 29th

sample	number of colonies
positive control pUC19	many colonies
transformation negative control	0
ligation without insert	0
3:1 insert:vector ratio	5
4:1 insert / vector ratio	5

Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)
==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name	Concentration (µg/mL)	Ratio 260/280
D187	16	1.31
D186	24	1.25

Ligation

Protocol

Ligation Name	Vector Name	Volume Vector (µL)	Insert	Volume Insert (µL)
L168	D187	2.5	D186	1
Control L168	D166	2.5	-

@@ Line 27: / Line 27: @@
-=Construction of pFlhB - mRFP Tripart (LVA+)=
+=Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)=
 Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-<br>We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
+<br>==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted
 ==Digestion==

Team:Paris/September 1

From 2008.igem.org

Latest revision as of 14:49, 13 September 2008

Contents

Cloning of EnvZ*

Transformation results of L165 (EnvZ*-pSB1A2) from August 29th

Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)

Digestion

Measurement of the concentration of D187 purified

Ligation