Team:Paris/September 10

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=Checking our ligases (following)=
=Checking our ligases (following)=
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==Analysis of the ligation transformants==
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*Several colonies for the pUC19 transformation control
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*No colonies for the ligations
==Analysis of the ligation products by electrophoresis==
==Analysis of the ligation products by electrophoresis==
[[Image:KR000310.JPG|thumb]]
[[Image:KR000310.JPG|thumb]]
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{|border="1" style="text-align: center"
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|'''well n°'''
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|1
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|2
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|3
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|4
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|5
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|6
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|7
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|8
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|9
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|10
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|11
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|12
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|13
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|14
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|15
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|-
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|'''sample'''
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|rowspan="5"|1 kb <br> DNA ladder
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|rowspan="5"|undigested DNA <br> MP101.1
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|colspan="12"|'''ligation products'''
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|rowspan="5"|100 bp <br> DNA ladder
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|-
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|'''temperature of ligation'''
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|colspan="8"|16°C
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|colspan="4"|4°C
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|-
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|'''ligase n°'''
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|colspan="4"|L1
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|colspan="4"|L2
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|colspan="2"|L1
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|colspan="2"|L2
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|-
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|'''duration of ligation'''
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|colspan="2"|2 h
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|colspan="2"|O/N
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|colspan="2"|2 h
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|colspan="6"|O/N
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|-
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|'''amount of ligase (units)'''
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|400 U
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|2 U
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|400 U
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|2 U
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|400 U
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|2 U
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|400 U
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|2 U
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|400 U
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|2 U
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|400 U
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|2 U
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|-
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|}

Latest revision as of 14:26, 12 September 2008

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Checking our ligases (following)

Analysis of the ligation transformants

  • Several colonies for the pUC19 transformation control
  • No colonies for the ligations

Analysis of the ligation products by electrophoresis

KR000310.JPG
well n° 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
sample 1 kb
DNA ladder 		undigested DNA
MP101.1 		ligation products 100 bp
DNA ladder
temperature of ligation 16°C 4°C
ligase n° L1 L2 L1 L2
duration of ligation 2 h O/N 2 h O/N
amount of ligase (units) 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U
Retrieved from "http://2008.igem.org/Team:Paris/September_10"